A transfection of Antisense Oligodeoxynucleotide (ODN) with Microporator Download

Steroids Production after Modulating Steroidogenic Acute Regulatory Protein
Gene Expression in an Immortalized Adrenocortical Steroidogenic Cell Line
Gi Hoon Son*, Sooyoung Chung, Hee-Dae Kim, Han Kyoung Choe and Kyungjin Kim
*Department of Biological Sciences, Seoul National University, Seoul 151-742, KOREA

Microporation technology, a highly efficient gene delivery method for hard-to-transfect cells Download

The increasing demand for efficient transfections reflects the expanding applications for transfection technology. Introducing foreign DNA into mammalian cells has become fundamental for analyzing gene function, producing recombinant gene products and devising strategies for gene therapy. Successful transfection results rely on the transfection method. However, transfection of hard-to-transfect cells (including primary neuron cells, primary blood cells, or mouse stem cells) through common methods such as calcium phosphate, liposome-based reagent, electroporation or viral vector is problematic because of low transfection efficiency and low cell survival rate. In contrast to conventional methods, Microporation’s novel electroporation technology offers these essential qualities for achieving successful transfection results. Microporation technology is capillary tip and pipette - based gene transfer technique, which lacks of a number of harmful effects of cuvette-based electroporation method such as pH variation, temperature rising, turbulence, and metal ion generation. Microporation provides an easier and more efficient method for hard-to-transfect cells with its high transfection efficiency and reproducibility. In our study, we have transfected 80 cell lines and 10 primary cells, including hart-to-transfect cells, using the Microporator. Most transfection results showed a final transfection efficiency of 70% average.