Multiple sclerosis (MS) is a potentially disabling disease of the central nervous system. Approximately half of the patients with MS experience severe pain; However, currently available therapies provide only enough assistance. The mechanisms underlying the generation of neuropathic pain in patients with MS is not fully understood. More recently, we found that the neutrophil elastase of the accumulation of neutrophils in mechanical allodynia dorsal root ganglion (DRG) neurons and induces sensitive DRG in a mouse model of experimental autoimmune encephalomyelitis (EAE).
However, the underlying mechanisms of accumulation of neutrophils in DRG after myelin oligodendrocyte glycoprotein (MOG35-55, an immunogenic peptide) immunization is still unclear. Here, we found that the C-X-C motif ligand 1 (CXCL1) is regulated in DRG neurons after MOG35-55 immunization.
Increased CXCL1 protein expression was also observed in primary cultured DRG neurons treated with MOG35-55, mediated by toll like receptor 4 (TLR4). TLR4 gene silencing or CXCL1 in DRG neurons was significantly attenuated neutrophil accumulation in the DRG and mechanical allodynia during the preclinical phase of EAE (around day 5 after immunization). Our results thus demonstrate that TLR4 pathway-CXCL1 in DRG neurons in DRG triggers neutrophil recruitment and subsequent mechanical allodynia in response to MOG35-55.
DAPI (hydrochloride) |
C3362-10 |
ApexBio |
10 mg |
EUR 122 |
DAPI (hydrochloride) |
C3362-25 |
ApexBio |
25 mg |
EUR 200 |
DAPI (hydrochloride) |
C3362-5 |
ApexBio |
5 mg |
EUR 93 |
DAPI Staining Kit |
abx097206-1Kit |
Abbexa |
1 Kit |
EUR 230 |
|
FluorLast? with DAPI |
1214-20 |
Biovision |
|
EUR 153 |
DAPI (Molecular Biology Grade) |
CE117 |
GeneOn |
5 mg |
EUR 60 |
DAPI (Molecular Biology Grade) |
CE118 |
GeneOn |
25 mg |
EUR 133 |
DAPI (Molecular Biology Grade) |
CE119 |
GeneOn |
100 mg |
EUR 319 |
EverBrite Mounting Medium with DAPI |
23002 |
Biotium |
10mL |
EUR 195 |
Description: Minimum order quantity: 1 unit of 10mL |
DAPI, 10mg/mL in H2O |
40043 |
Biotium |
1ML |
EUR 134 |
Description: Minimum order quantity: 1 unit of 1ML |
FluorLast? with DAPI and DABCO |
1218-20 |
Biovision |
|
EUR 153 |
FluoroQuest™ Mounting Medium with DAPI |
20004 |
AAT Bioquest |
50 mL |
EUR 132 |
|
EverBrite Hardset Mounting Medium with DAPI |
23004 |
Biotium |
10mL |
EUR 213 |
Description: Minimum order quantity: 1 unit of 10mL |
Anti-Fade Mounting Medium (With DAPI) |
abx090690-10ml |
Abbexa |
10 ml |
EUR 203 |
|
DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) |
B551 |
ABM |
0.2 ml |
EUR 108 |
DAPI, dilactate (4’, 6-diamidino-2-phenylindole, dilactate) |
40009 |
Biotium |
10MG |
EUR 146 |
Description: Minimum order quantity: 1 unit of 10MG |
DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3* |
17510 |
AAT Bioquest |
10 mg |
EUR 115 |
|
DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3* |
17511 |
AAT Bioquest |
100 mg |
EUR 219 |
|
DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3* |
17513 |
AAT Bioquest |
25 mg |
EUR 132 |
|
Human 4’,6-Diamidino-2-phenylindole dihydrochloride(DAPI)ELISA kit |
QY-E05538 |
Qayee Biotechnology |
96T |
EUR 361 |
DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *10 mM solution in water* |
17507 |
AAT Bioquest |
2 mL |
EUR 115 |
|
DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining |
AR1176 |
BosterBio |
10mL(100 assys) |
EUR 65 |
DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining |
AR1177 |
BosterBio |
50mL(500 assys) |
EUR 101 |