The Prenylflavonoid ENDF1 Overrules Central Nervous System Growth Inhibitors and Facilitates Regeneration of DRG Neurons.

Restoration of neuronal connectivity after a lesion of the central nervous system, such as spinal cord injury, is one of the greatest challenges in modern medicine. In particular, the accumulation of factors inhibiting the growth of axons at the site of injury is a major obstacle to the improvement of structural and thus functional.

We previously investigated prenylflavonoids group derived from hops to their capacity to promote neuroregeneration. We identified a molecule called ENDF1 a very powerful tool to increase the re-growth and branching of neurites of dorsal root ganglion neurons in culture in promoting the growth substrate.

In this study, we investigated the capacity ENDF1 to promote regeneration of rat dorsal root ganglion neurons in vitro in the presence of the three main components of the extracellular matrix that acts as an inhibitor of axon growth: semaphorin 3A, Ephrin A4 and mixed with chondroitin sulfate proteoglycans. We reported that ENDF1 application significantly promoted the percentage of sensory neurons were able to regrow their neurites irrespective of their presence inhibitor, and this to an extent similar to that obtained after NGF treatment. In addition, the greatly enhanced ENDF1 total neurite length and complexity of neurites extending from neurons challenged with axon growth inhibitors.

Although the effects of NGF and ENDF1 on regeneration of neurons was similar, ENDF1 activity is not mediated by signaling through TrkA receptor, suggesting that each molecule acts via different signaling pathways. Additionally, ENDF1 not degrade cofilin phosphorylation, downstream effectors associated regeneration of RhoA / ROCK signaling pathway. Therefore, ENDF1 is pro-neuroregenerative potent factor that may help in identifying new efficient targets for regenerative therapies of the nervous system.

UltraTek HRP Anti-Rabbit (DAB) Staining System
AKC080	ScyTek Laboratories	70 Slides	EUR 152.4

SensiTek HRP Anti-Polyvalent (DAB) Staining System
AEM080	ScyTek Laboratories	70 Slides	EUR 152.4

UltraTek HRP Anti-Polyvalent (DAB) Staining System
AMF080	ScyTek Laboratories	70 Slides	EUR 165.6

STAT-Q Multivalent DAB KIT for staining Mouse, Rat
NB314KLD	Innovex	1000 slides	EUR 895
Description: STAT-Q Multivalent DAB KIT for staining Mouse, Rat and Rabbit antibodies, 1000 plus slides (Large size kit)

STAT-Q Multivalent DAB KIT for staining Mouse, Rat
NB314KLD-20	Innovex	300 slide kit	EUR 595
Description: STAT-Q Multivalent DAB KIT for staining Mouse, Rat and Rabbit antibodies, 350 slides kit (Small size Kit)

STAT-Q Goat DAB KIT for staining all Goat primary
NB314KLGD-20	Innovex	20 ml kit	EUR 589
Description: STAT-Q Goat DAB KIT for staining all Goat primary antibodies, 350 plus slides

STAT-Q Rabbit DAB KIT for staining all Rabbit anti
NB314KLRD-20	Innovex	20 ml kit	EUR 585
Description: STAT-Q Rabbit DAB KIT for staining all Rabbit antibodies, 350 plus slides

STAT-Q Mouse Adsorbed DAB KIT for staining Rat ant
NB314MAK-DAB	Innovex	200-250 slides	EUR 695
Description: STAT-Q Mouse Adsorbed DAB KIT for staining Rat antibodies-on-Mouse tissues 250 plus slide kit

STAT-Q Rat Adsorbed DAB KIT for staining Mouse ant
NB314RAK-DAB	Innovex	200-250 slides	EUR 695
Description: STAT-Q Rat Adsorbed DAB KIT for staining Mouse antibodies-on-Rat tissues 250 plus slides kit

STAT Double Staining ragent for Double staining Mo
NB330DB	Innovex	25 ml	EUR 595
Description: STAT Double Staining ragent for Double staining Mouse and Sheep antibodies, 450 Plus slides

STAT Double Staining reagent for Double staining o
NB326DB	Innovex	25 ml	EUR 595
Description: STAT Double Staining reagent for Double staining of Mouse and Rabbit antibodies, 450 plus slides

STAT Double Staining Reagent for Double staining o
NB327DB	Innovex	25 ml	EUR 595
Description: STAT Double Staining Reagent for Double staining of Mouse and Goat antibodies, 450 plus slides

PI Staining Kit
abx097208-1Kit	Abbexa	1 Kit	EUR 276

DAPI Staining Kit
abx097206-1Kit	Abbexa	1 Kit	EUR 276

Hoechst 33342 Staining Kit
abx097207-1Kit	Abbexa	1 Kit	EUR 276

?-Galactosidase Staining Kit
AKR-100	Cell Biolabs	1 kit	EUR 518.4
Description: This simple assay measures the transfection efficiency of the LacZ gene using a simple staining protocol.

PI / RNase Staining Buffer
20-abx090619	Abbexa	EUR 326.40 EUR 276.00	100 tests 50 tests

ProsiBlue Gel Staining Solution
P7300-050	GenDepot	500ml	EUR 208.8

Ponceau S Staining Solution
B7778-100000	ApexBio	100 g	EUR 195.6

Ponceau S Staining Solution
B7778-50000	ApexBio	50 g	EUR 151.2

SDS-PAGE Staining Solution
GR103068	Genorise Scientific	100 mL	EUR 58

Ponceau S Staining Solution
PW005	Bio Basic	100ml	EUR 82.97

Dual- Color AP Staining Kit
AP100D-1	SBI	100 Assays	EUR 494.4

EasyDip Slide Staining System
M900-12AS-ASSORTED	ScyTek Laboratories	1 Case(s)	EUR 211.2

Red-Color AP Staining Kit
AP100R-1	SBI	50 Assays	EUR 314.4

Live/Dead Cell Staining Kit
55R-1434	Fitzgerald	100 tests	EUR 765.6
Description: Live/Dead Cell Staining Kit for use in the research laboratory

PAP PEN FOR IMMUNO STAINING
Z377821-1EA	Scientific Laboratory Supplies	EACH	EUR 171.03

Stain Tray Slide Staining System
M920-1	ScyTek Laboratories	1 Clear	EUR 513.6

Stain Tray Slide Staining System
M920-2	ScyTek Laboratories	1 Black	EUR 534

CometAssay Silver Staining Kit
4254-200-K	Biotechne	200 Tests	EUR 390.96

Nalgene Box Staining 13x13x5cm
BOX1040	Scientific Laboratory Supplies	EACH	EUR 48

Blue-Color AP Staining Kit
AP100B-1	SBI	50 Assays	EUR 203

Beta Galactosidase Staining Kit
55R-1541	Fitzgerald	250 assays	EUR 444
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

Disposable Slide Staining Trays
MIC5070	Scientific Laboratory Supplies	PK4	EUR 90

Staining Trough+Lid Rectangular
MIC6008	Scientific Laboratory Supplies	EACH	EUR 27.6

Live-Dead Cell Staining Kit
K501-100	Biovision	each	EUR 561.6

Live-Dead Cell Staining Kit
K2081-100	ApexBio	100 staining	EUR 552

Wheaton staining dishes 3 EA
S5017-3EA	Scientific Laboratory Supplies	PK3	EUR 135.6

Staining Jar PP + Lid 10 Slide
MIC6002	Scientific Laboratory Supplies	EACH	EUR 67.2

Purple-Color AP Staining Kit
AP100P-1	SBI	100 Assays	EUR 235

Nerve Terminal Staining Kit V
70034	Biotium	1SET	EUR 610.8
Description: Minimum order quantity: 1 unit of 1SET

Human Histochemistry Staining Kit
20-abx299039	Abbexa	EUR 1045.20 EUR 1462.80 EUR 678.00	150 tests 500 tests 50 tests

Beta-Galactosidase Staining Kit
K802-250	Biovision	each	EUR 352.8

Alkaline Phosphatase Staining Kit
K2035-50	Biovision	50 assays	EUR 454.8

Beta-Galactosidase Staining Kit
K2182-250	ApexBio	250 assays	EUR 351.6

Lipid (Oil Red O) staining Kit
K580-24	Biovision	each	EUR 542.4

Staining Jar W Lid 115x115x77mm
MIC6016	Scientific Laboratory Supplies	PK10	EUR 428.4

Manual Staining System (12 Wells)
IMS001	ScyTek Laboratories	1 ea.	EUR 427.2

Red Active Caspase Staining Kit
K2052-100	ApexBio	100 assays	EUR 686.4

Red Active Caspase Staining Kit
K2052-25	ApexBio	25 assays	EUR 285.6

Alcian Blue (pH 2.5) Staining Kit
AFR-1	ScyTek Laboratories	250 ml ea.	EUR 249.6

Alcian Blue (pH 2.5) Staining Kit
AFR-2	ScyTek Laboratories	30 ml ea.	EUR 153.6

DNA Ploidy Analysis Staining Kit
K1439-1	Biovision	each	EUR 705.6

DNA Ploidy Analysis Staining Kit
K1439-5	Biovision	each	EUR 2402.4

Staining Dish with Cover Plastic
X24085	Scientific Laboratory Supplies	EACH	EUR 156

7-AAD Viability Staining Buffer
abx090617-100tests	Abbexa	100 tests	EUR 260.4

Simport EasyDip Staining Jar Blue
HIS0273	Scientific Laboratory Supplies	PK6	EUR 117.6

Simport EasyDip Staining Jar Pink
HIS0275	Scientific Laboratory Supplies	PK6	EUR 117.6

Stayrightâ„¢ Purple HRP Staining Kit
45905-5mL	AAT Bioquest	5 mL	EUR 109
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit
45906-50mL	AAT Bioquest	50 mL	EUR 334
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Simport EasyDip Staining Jar Green
HIS0274	Scientific Laboratory Supplies	PK6	EUR 117.6

Simport EasyDip Staining Jar White
HIS0276	Scientific Laboratory Supplies	PK6	EUR 110.4

Simport EasyDip Staining Rack Grey
HIS0278	Scientific Laboratory Supplies	PK6	EUR 128.4

Protein Stain-EZ J Phosphoprotein Staining Kit
BSP043	Bio Basic	2x10minigel, 20prep	EUR 133.08

Hoechst 33342 / PI Double Staining Kit
abx097209-1Kit	Abbexa	1 Kit	EUR 292.8

7-AAD Viability Staining Solution
EXB0026	ExBio	400 tests	EUR 91.2

Simport EasyDip Staining Jar Yellow
HIS0277	Scientific Laboratory Supplies	PK6	EUR 110.4

SafePinky DNA Gel Staining Solution
S1000-050	GenDepot	500ml	EUR 157.2

SafePinky DNA Gel Staining Solution
S1000-100	GenDepot	2X500ml	EUR 225.6

SafePinky DNA Gel Staining Solution
S1000-200	GenDepot	4X500ml	EUR 336

CaspGLOW Active Caspase Staining Kit
55R-1292	Fitzgerald	25 assays	EUR 355.2
Description: Caspase Staining Kit for use activity in the research laboratory

Mouse to Mouse HRP Staining System
MTM003	ScyTek Laboratories	400 Slides	EUR 433.2

Coomassie Blue Fast Staining Solution
AR0170	BosterBio	100mL(for 10 assays to stain gels of 5 x 8.5cm)	EUR 96

Staining Jar Schiefferdecker With Lid
MIC6014	Scientific Laboratory Supplies	EACH	EUR 22.8

Human Type I Collagen Staining Kit
9044	Chondrex	1 kit	EUR 406.26
Description: Human Type I Collagen Staining Kit

Red Active Caspase-3 Staining Kit
K2053-100	ApexBio	100 assays	EUR 686.4

Red Active Caspase-3 Staining Kit
K2053-25	ApexBio	25 assays	EUR 285.6

Red Active Caspase-8 Staining Kit
K2054-100	ApexBio	100 assays	EUR 686.4

Red Active Caspase-8 Staining Kit
K2054-25	ApexBio	25 assays	EUR 285.6

Red Active Caspase-9 Staining Kit
K2055-100	ApexBio	100 assays	EUR 686.4

Red Active Caspase-9 Staining Kit
K2055-25	ApexBio	25 assays	EUR 285.6

Stayright™ Purple HRP Staining Kit
45905	AAT Bioquest	5 mL	EUR 109

Stayright™ Purple HRP Staining Kit
45906	AAT Bioquest	50 mL	EUR 334

Hellandahl Staining Jar+Lid 16 slide
MIC6004	Scientific Laboratory Supplies	EACH	EUR 34.8

Fluorescein Active Caspase Staining Kit
K2047-100	ApexBio	100 assays	EUR 686.4

Fluorescein Active Caspase Staining Kit
K2047-25	ApexBio	25 assays	EUR 285.6

Coplin Staining Jar Glass+Lid 10Slide
MIC6000	Scientific Laboratory Supplies	EACH	EUR 13.2

BRAND(TM) STAINING TRAY FOR 25 SLIDES
BR471400-5EA	Scientific Laboratory Supplies	PK5	EUR 58.1

BRAND(TM) STAINING TRAY FOR 20 SLIDES
BR474305-2EA	Scientific Laboratory Supplies	PK2	EUR 43.2

Prosi Silver Staining Kit for 25 mini gel
S3200-020	GenDepot	1kit	EUR 433.2

Yeast Viability Staining Kit (Green/Red)
31063-1	Biotium	1kit	EUR 566.4
Description: Minimum order quantity: 1 unit of 1kit

Gel-FAST? Gel Staining/Destaining Kit
K901-40	Biovision	each	EUR 210

Vertical Slide Staining Rack Dark Gray
M905-12DGY	ScyTek Laboratories	1 Case(s)	EUR 220.8

CaspGLOW Active Caspase 12 Staining Kit
55R-1291	Fitzgerald	25 assays	EUR 338.4
Description: Caspase 12 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 2 Staining Kit
55R-1293	Fitzgerald	25 assays	EUR 340.8
Description: Caspase 2 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 3 Staining Kit
55R-1294	Fitzgerald	25 assays	EUR 355.2
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 8 Staining Kit
55R-1295	Fitzgerald	25 assays	EUR 355.2
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 9 Staining Kit
55R-1296	Fitzgerald	25 assays	EUR 355.2
Description: Caspase 9 Staining Kit for use activity in the research laboratory

dsGreen Gel Staining Solution, 10000×, 0.5 mL
10010	Lumiprobe	0.5 mL	EUR 106.8

dsGreen Gel Staining Solution, 10000×, 50 uL
A0010	Lumiprobe	50 uL	EUR 62.4

Mouse to Mouse HRP (AEC) Staining System
MTM002	ScyTek Laboratories	70 Slides	EUR 188.4

CaspGLOW Red Active Caspase Staining Kit
55R-1297	Fitzgerald	25 assays	EUR 355.2
Description: Caspase Staining Kit for use activity in the research laboratory

Coomassie Blue G-250 Staining Solution
GR103035	Genorise Scientific	1 L	EUR 95

CaspGLOW? Red Active Caspase Staining Kit
K190-100	Biovision	each	EUR 627.6

CaspGLOW? Red Active Caspase Staining Kit
K190-25	Biovision	each	EUR 288

Coomassie Blue Staining Destaining Solution
AR0140	BosterBio	1kit (for 10 assays to stain gels of 5 x 8.5cm)	EUR 84

Coomassie Brilliant Blue Gel Staining Kit
abx090653-1Kit	Abbexa	1 Kit	EUR 226.8

SensiTek HRP Anti-Mouse Staining System
AFA600	ScyTek Laboratories	500 Slides	EUR 258

UltraTek HRP Anti-Mouse Staining System
AFJ600	ScyTek Laboratories	500 Slides	EUR 340.8

Staining Jar 4ml Cavity 40x40mm With lid
MIC6006	Scientific Laboratory Supplies	EACH	EUR 8.4

SensiTek HRP Anti-Rabbit Staining System
AFE600	ScyTek Laboratories	500 Slides	EUR 258

UltraTek HRP Anti-Rabbit Staining System
AFK600	ScyTek Laboratories	500 Slides	EUR 340.8

Mouse to Mouse Alk-Phos Staining System
MTM005	ScyTek Laboratories	400 Slides	EUR 433.2

Fluorescein Active Caspase-2 Staining Kit
K2048-100	ApexBio	100 assays	EUR 686.4

Fluorescein Active Caspase-2 Staining Kit
K2048-25	ApexBio	25 assays	EUR 285.6

Fluorescein Active Caspase-3 Staining Kit
K2049-100	ApexBio	100 assays	EUR 686.4

Fluorescein Active Caspase-3 Staining Kit
K2049-25	ApexBio	25 assays	EUR 285.6

Fluorescein Active Caspase-8 Staining Kit
K2050-100	ApexBio	100 assays	EUR 686.4

Fluorescein Active Caspase-8 Staining Kit
K2050-25	ApexBio	25 assays	EUR 285.6

Fluorescein Active Caspase-9 Staining Kit
K2051-100	ApexBio	100 assays	EUR 686.4

Fluorescein Active Caspase-9 Staining Kit
K2051-25	ApexBio	25 assays	EUR 285.6

Fluorescein Active Caspase-12 Staining Kit
K2046-100	ApexBio	100 assays	EUR 686.4

Fluorescein Active Caspase-12 Staining Kit
K2046-25	ApexBio	25 assays	EUR 285.6

Safe E-Red DNA Gel Staining Solution(1000x)
S1002-001	GenDepot	1ml	EUR 133.2

Safe E-Red DNA Gel Staining Solution(1000x)
S1002-005	GenDepot	5x1ml	EUR 271.2

Safe E-Red DNA Gel Staining Solution(1000x)
S1002-010	GenDepot	10ml	EUR 360

Safe E-Red DNA Gel Staining Solution(1000x)
S1002-020	GenDepot	20ml	EUR 595.2

CaspGLOW Red Active Caspase 3 Staining Kit
55R-1298	Fitzgerald	25 assays	EUR 355.2
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase 8 Staining Kit
55R-1299	Fitzgerald	25 assays	EUR 355.2
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase 9 Staining Kit
55R-1300	Fitzgerald	25 assays	EUR 355.2
Description: Caspase 9 Staining Kit for use activity in the research laboratory

Geliteâ„¢ Green Nucleic Acid Gel Staining Kit
17589-1Kit	AAT Bioquest	1 Kit	EUR 222
Description: Gelite™ Green is a sensitive fluorescent nucleic acid gel stain for detecting nucleic acids in agarose and polyacrylamide gels.

Sirius Red/Fast Green Collagen Staining Kit
9046	Chondrex	30-50 samples	EUR 164.4
Description: Sirius Red/Fast Green Collagen Staining Kit

StemTAG Alkaline Phosphatase Staining Kit (Red)
CBA-300	Cell Biolabs	100 assays	EUR 553.2
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

Human FoxP3 Transcription Factor Staining Kit
OKTA00001	Aviva Systems Biology	KIT	EUR 544.8
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

Human FoxP3 Transcription Factor Staining Kit
OKTA00004	Aviva Systems Biology	KIT	EUR 544.8
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

Basic Fuchsin, for flagella staining, certified
GT2106-25G	Glentham Life Sciences	25 g	EUR 274.8

Genorise® Alkaline Phosphatase Staining Kit
GR107045	Genorise Scientific	100 tests	EUR 289

Basic Fuchsin, for flagella staining, certified
GT2106-25	Glentham Life Sciences	25	EUR 197.7

Differentiation Solution for Regressive Staining
GE3953-1	Glentham Life Sciences	1	EUR 52.8

CaspGLOW? Red Active Caspase-3 Staining Kit
K193-100	Biovision	each	EUR 639.6

CaspGLOW? Red Active Caspase-3 Staining Kit
K193-25	Biovision	each	EUR 288

CaspGLOW? Red Active Caspase-9 Staining Kit
K199-100	Biovision	each	EUR 620.4

CaspGLOW? Red Active Caspase-9 Staining Kit
K199-25	Biovision	each	EUR 288

CaspGLOW? Red Active Caspase-8 Staining Kit
K198-100	Biovision	each	EUR 627.6

CaspGLOW? Red Active Caspase-8 Staining Kit
K198-25	Biovision	each	EUR 288

Geliteâ„¢ Orange Nucleic Acid Gel Staining Kit
17594-1Kit	AAT Bioquest	1 Kit	EUR 222
Description: Gelite™ Orange is an extremely sensitive nucleic acid gel stain for detecting DNA or RNA in gels using a standard 300 nm UV transilluminator and Polaroid 667 black-and-white print film.

SensiTek HRP Anti-Polyvalent Staining System
AFG600	ScyTek Laboratories	500 Slides	EUR 444

UltraTek HRP Anti-Polyvalent Staining System
AFN600	ScyTek Laboratories	500 Slides	EUR 340.8

SensiTek HRP Anti-Mouse (AEC) Staining System
AEB080	ScyTek Laboratories	70 Slides	EUR 142.8

UltraTek HRP Anti-Mouse (AEC) Staining System
AKB080	ScyTek Laboratories	70 Slides	EUR 152.4

Type IX Collagen Staining Kit, Multi-Species
9012	Chondrex	1 kit	EUR 406.26
Description: Type IX Collagen Staining Kit

Blue Feulgen DNA Ploidy Analysis Staining Kit
DPK500	ScyTek Laboratories	1 kit(s)	EUR 427.2

Coomassie Brilliant Blue Fast Staining Solution
abx090652-200ml	Abbexa	200 ml	EUR 243.6

SensiTek HRP Anti-Rabbit (AEC) Staining System
AEJ080	ScyTek Laboratories	70 Slides	EUR 142.8

UltraTek HRP Anti-Rabbit (AEC) Staining System
AKD080	ScyTek Laboratories	70 Slides	EUR 152.4