Restoration of neuronal connectivity after a lesion of the central nervous system, such as spinal cord injury, is one of the greatest challenges in modern medicine. In particular, the accumulation of factors inhibiting the growth of axons at the site of injury is a major obstacle to the improvement of structural and thus functional.
We previously investigated prenylflavonoids group derived from hops to their capacity to promote neuroregeneration. We identified a molecule called ENDF1 a very powerful tool to increase the re-growth and branching of neurites of dorsal root ganglion neurons in culture in promoting the growth substrate.
In this study, we investigated the capacity ENDF1 to promote regeneration of rat dorsal root ganglion neurons in vitro in the presence of the three main components of the extracellular matrix that acts as an inhibitor of axon growth: semaphorin 3A, Ephrin A4 and mixed with chondroitin sulfate proteoglycans. We reported that ENDF1 application significantly promoted the percentage of sensory neurons were able to regrow their neurites irrespective of their presence inhibitor, and this to an extent similar to that obtained after NGF treatment. In addition, the greatly enhanced ENDF1 total neurite length and complexity of neurites extending from neurons challenged with axon growth inhibitors.
Although the effects of NGF and ENDF1 on regeneration of neurons was similar, ENDF1 activity is not mediated by signaling through TrkA receptor, suggesting that each molecule acts via different signaling pathways. Additionally, ENDF1 not degrade cofilin phosphorylation, downstream effectors associated regeneration of RhoA / ROCK signaling pathway. Therefore, ENDF1 is pro-neuroregenerative potent factor that may help in identifying new efficient targets for regenerative therapies of the nervous system.
UltraTek HRP Anti-Rabbit (DAB) Staining System |
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AKC080 | ScyTek Laboratories | 70 Slides | EUR 152.4 |
SensiTek HRP Anti-Polyvalent (DAB) Staining System |
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AEM080 | ScyTek Laboratories | 70 Slides | EUR 152.4 |
UltraTek HRP Anti-Polyvalent (DAB) Staining System |
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AMF080 | ScyTek Laboratories | 70 Slides | EUR 165.6 |
STAT-Q Multivalent DAB KIT for staining Mouse, Rat |
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NB314KLD | Innovex | 1000 slides | EUR 895 |
Description: STAT-Q Multivalent DAB KIT for staining Mouse, Rat and Rabbit antibodies, 1000 plus slides (Large size kit) |
STAT-Q Multivalent DAB KIT for staining Mouse, Rat |
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NB314KLD-20 | Innovex | 300 slide kit | EUR 595 |
Description: STAT-Q Multivalent DAB KIT for staining Mouse, Rat and Rabbit antibodies, 350 slides kit (Small size Kit) |
STAT-Q Goat DAB KIT for staining all Goat primary |
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NB314KLGD-20 | Innovex | 20 ml kit | EUR 589 |
Description: STAT-Q Goat DAB KIT for staining all Goat primary antibodies, 350 plus slides |
STAT-Q Rabbit DAB KIT for staining all Rabbit anti |
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NB314KLRD-20 | Innovex | 20 ml kit | EUR 585 |
Description: STAT-Q Rabbit DAB KIT for staining all Rabbit antibodies, 350 plus slides |
STAT-Q Mouse Adsorbed DAB KIT for staining Rat ant |
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NB314MAK-DAB | Innovex | 200-250 slides | EUR 695 |
Description: STAT-Q Mouse Adsorbed DAB KIT for staining Rat antibodies-on-Mouse tissues 250 plus slide kit |
STAT-Q Rat Adsorbed DAB KIT for staining Mouse ant |
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NB314RAK-DAB | Innovex | 200-250 slides | EUR 695 |
Description: STAT-Q Rat Adsorbed DAB KIT for staining Mouse antibodies-on-Rat tissues 250 plus slides kit |
STAT Double Staining ragent for Double staining Mo |
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NB330DB | Innovex | 25 ml | EUR 595 |
Description: STAT Double Staining ragent for Double staining Mouse and Sheep antibodies, 450 Plus slides |
STAT Double Staining reagent for Double staining o |
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NB326DB | Innovex | 25 ml | EUR 595 |
Description: STAT Double Staining reagent for Double staining of Mouse and Rabbit antibodies, 450 plus slides |
STAT Double Staining Reagent for Double staining o |
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NB327DB | Innovex | 25 ml | EUR 595 |
Description: STAT Double Staining Reagent for Double staining of Mouse and Goat antibodies, 450 plus slides |
PI Staining Kit |
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abx097208-1Kit | Abbexa | 1 Kit | EUR 276 |
DAPI Staining Kit |
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abx097206-1Kit | Abbexa | 1 Kit | EUR 276 |
TRAP Staining Kit |
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KT-008 | Kamiya Biomedical Company | 1 kit | EUR 747 |
Coplin Staining Jar |
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08415-3 | Polysciences Europe GmbH | 3sets | EUR 84 |
Cell Staining Buffer |
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E-CK-A107-100mL | Elabscience Biotech | 100 mL | EUR 20 |
Cell Staining Buffer |
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E-CK-A107-200mL | Elabscience Biotech | 200 mL | EUR 35 |
Cell Staining Buffer |
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E-CK-A107-500mL | Elabscience Biotech | 500 mL | EUR 60 |
Cell Staining Buffer |
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E16FXP005 | EnoGene | 500 ml | EUR 40 |
Description: Available in various conjugation types. |
ELVIS ID Staining Kit |
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SK-ELVIS-100 | Quidel | 100 Tests | Ask for price |
ELVIS ID Staining Kit |
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SK-ELVIS-1000 | Quidel | 1000 Tests | Ask for price |
ELVIS ID Staining Kit |
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SK-ELVIS-200 | Quidel | 200 Tests | Ask for price |
ELVIS ID Staining Kit |
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SK-ELVIS-500 | Quidel | 500 Tests | Ask for price |
Hoechst 33342 Staining Kit |
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abx097207-1Kit | Abbexa | 1 Kit | EUR 276 |
?-Galactosidase Staining Kit |
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AKR-100 | Cell Biolabs | 1 kit | EUR 518.4 |
Description: This simple assay measures the transfection efficiency of the LacZ gene using a simple staining protocol. |
Cell Cycle Staining Kit |
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KTA2020-100T | Abbkine | 100 T | EUR 119 |
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle. |
Cell Cycle Staining Kit |
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KTA2020-50T | Abbkine | 50 T | EUR 69 |
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle. |
PI / RNase Staining Buffer |
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20-abx090619 | Abbexa |
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Live NucRed staining kit |
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EGY069 | EnoGene | >100T | EUR 210 |
HISTOCHEMISTRY STAINING TRAY |
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AT-12 | IBI Scientific | 12 PLACE - BLACK LID | EUR 226.1 |
HISTOCHEMISTRY STAINING TRAY |
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AT-24 | IBI Scientific | 24 PLACE - BLACK LID | EUR 342.21 |
HISTOCHEMISTRY STAINING TRAY |
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ATC-12 | IBI Scientific | 12 PLACE - CLEAR LID | EUR 226.1 |
HISTOCHEMISTRY STAINING TRAY |
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ATC-24 | IBI Scientific | 24 PLACE - CLEAR LID | EUR 342.21 |
Exosome fluorescent staining |
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S104 | 101Bio | - | EUR 800 |
ProsiBlue Gel Staining Solution |
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P7300-050 | GenDepot | 500ml | EUR 208.8 |
Stains All Gel Staining Kit |
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KT-792 | Kamiya Biomedical Company | 20 tests | EUR 737 |
Live NucGreen staining kit |
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EGY068 | EnoGene | >100T | EUR 210 |
Dead NucGreen staining kit |
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EGY070 | EnoGene | >100T | EUR 210 |
GEL STAINING/INCUBATION BOX |
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SB-10 | IBI Scientific | LIGHT SHIELDING | EUR 94.44 |
Ponceau S Staining Solution |
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B7778-100000 | ApexBio | 100 g | EUR 195.6 |
Ponceau S Staining Solution |
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B7778-50000 | ApexBio | 50 g | EUR 151.2 |
SDS-PAGE Staining Solution |
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GR103068 | Genorise Scientific | 100 mL | EUR 58 |
Ponceau S Staining Solution |
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PW005 | Bio Basic | 100ml | EUR 82.97 |
Ponceau S Staining Solution |
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41640009-1 | Bio-WORLD | 500 mL | EUR 40.13 |
Ponceau S Staining Solution |
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41640009-2 | Bio-WORLD | 1 L | EUR 58.16 |
Dual- Color AP Staining Kit |
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AP100D-1 | SBI | 100 Assays | EUR 494.4 |
EasyDip Slide Staining System |
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M900-12AS-ASSORTED | ScyTek Laboratories | 1 Case(s) | EUR 211.2 |
Red-Color AP Staining Kit |
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AP100R-1 | SBI | 50 Assays | EUR 314.4 |
Live/Dead Cell Staining Kit |
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55R-1434 | Fitzgerald | 100 tests | EUR 765.6 |
Description: Live/Dead Cell Staining Kit for use in the research laboratory |
PAP PEN FOR IMMUNO STAINING |
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Z377821-1EA | Scientific Laboratory Supplies | EACH | EUR 171.03 |
CometAssay Silver Staining Kit |
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4254-200-K | Biotechne | 200 Tests | EUR 390.96 |
Nalgene Box Staining 13x13x5cm |
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BOX1040 | Scientific Laboratory Supplies | EACH | EUR 48 |
PI Viability Staining Solution |
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E16FXP002 | EnoGene | 150 tests | EUR 40 |
Description: Available in various conjugation types. |
Blue-Color AP Staining Kit |
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AP100B-1 | SBI | 50 Assays | EUR 203 |
Beta Galactosidase Staining Kit |
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55R-1541 | Fitzgerald | 250 assays | EUR 444 |
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory |
Disposable Slide Staining Trays |
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MIC5070 | Scientific Laboratory Supplies | PK4 | EUR 90 |
Staining Trough+Lid Rectangular |
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MIC6008 | Scientific Laboratory Supplies | EACH | EUR 27.6 |
Live-Dead Cell Staining Kit |
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K501-100 | Biovision | each | EUR 561.6 |
Live-Dead Cell Staining Kit |
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K2081-100 | ApexBio | 100 staining | EUR 552 |
Wheaton staining dishes 3 EA |
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S5017-3EA | Scientific Laboratory Supplies | PK3 | EUR 135.6 |
Live-Dead Cell Staining Kit |
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K2081-1000 | ApexBio | 1000T | EUR 140 |
Live-Dead Cell Staining Kit |
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K2081-500 | ApexBio | 500T | EUR 85 |
EZ-Quick Slide Staining Set |
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IW-2510 | IHC World | - | EUR 425 |
Staining Jar PP + Lid 10 Slide |
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MIC6002 | Scientific Laboratory Supplies | EACH | EUR 67.2 |
Purple-Color AP Staining Kit |
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AP100P-1 | SBI | 100 Assays | EUR 235 |
Nerve Terminal Staining Kit V |
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70034 | Biotium | 1SET | EUR 610.8 |
Description: Minimum order quantity: 1 unit of 1SET |
Nerve Terminal Staining Kit I |
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70030-1 | Biotium | KT | EUR 605 |
Nerve Terminal Staining Kit V |
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70034-1 | Biotium | KT | EUR 605 |
Human Histochemistry Staining Kit |
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20-abx299039 | Abbexa |
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Beta-Galactosidase Staining Kit |
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K802-250 | Biovision | each | EUR 352.8 |
Alkaline Phosphatase Staining Kit |
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K2035-50 | Biovision | 50 assays | EUR 454.8 |
Beta-Galactosidase Staining Kit |
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K2182-250 | ApexBio | 250 assays | EUR 351.6 |
Staining Jar W Lid 115x115x77mm |
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MIC6016 | Scientific Laboratory Supplies | PK10 | EUR 428.4 |
Manual Staining System (12 Wells) |
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IMS001 | ScyTek Laboratories | 1 ea. | EUR 427.2 |
Red Active Caspase Staining Kit |
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K2052-100 | ApexBio | 100 assays | EUR 686.4 |
Red Active Caspase Staining Kit |
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K2052-25 | ApexBio | 25 assays | EUR 285.6 |
Nerve Terminal Staining Kit IIB |
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70031-1-1 | Biotium | KT | EUR 395 |
Nerve Terminal Staining Kit III |
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70032-1 | Biotium | KT | EUR 513 |
Lipid (Oil Red O) staining Kit |
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K580-24 | Biovision | each | EUR 542.4 |
Protein Stain-EZ J Phosphoprotein Staining Kit |
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BSP043 | Bio Basic | 2x10minigel, 20prep | EUR 133.08 |
DNA Ploidy Analysis Staining Kit |
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K1439-1 | Biovision | each | EUR 705.6 |
DNA Ploidy Analysis Staining Kit |
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K1439-5 | Biovision | each | EUR 2402.4 |
Staining Dish with Cover Plastic |
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X24085 | Scientific Laboratory Supplies | EACH | EUR 156 |
Alcian Blue (pH 2.5) Staining Kit |
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AFR-1 | ScyTek Laboratories | 250 ml ea. | EUR 249.6 |
Alcian Blue (pH 2.5) Staining Kit |
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AFR-2 | ScyTek Laboratories | 30 ml ea. | EUR 153.6 |
DAPI staining kit (ready to use) |
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EGY0621 | EnoGene | 10mL | EUR 85 |
DAPI staining kit (ready to use) |
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EGY0622 | EnoGene | 50mL | EUR 185 |
7-AAD Viability Staining Buffer |
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abx090617-100tests | Abbexa | 100 tests | EUR 260.4 |
Simport EasyDip Staining Jar Blue |
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HIS0273 | Scientific Laboratory Supplies | PK6 | EUR 117.6 |
Simport EasyDip Staining Jar Pink |
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HIS0275 | Scientific Laboratory Supplies | PK6 | EUR 117.6 |
Plastic Coplin Staining Jars, each |
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IW-2501 | IHC World | - | EUR 8.6 |
Stayrightâ„¢ Purple HRP Staining Kit |
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45905-5mL | AAT Bioquest | 5 mL | EUR 109 |
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications. |
Stayrightâ„¢ Purple HRP Staining Kit |
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45906-50mL | AAT Bioquest | 50 mL | EUR 334 |
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications. |
Silver protein for bodian staining 7.5-8.5% Ag |
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S03380 | Pfaltz & Bauer | 25G | EUR 634.45 |
Simport EasyDip Staining Jar Green |
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HIS0274 | Scientific Laboratory Supplies | PK6 | EUR 117.6 |
Simport EasyDip Staining Jar White |
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HIS0276 | Scientific Laboratory Supplies | PK6 | EUR 110.4 |
Simport EasyDip Staining Rack Grey |
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HIS0278 | Scientific Laboratory Supplies | PK6 | EUR 128.4 |
Hematoxylin and Eosin Staining Kit |
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K1142-1000 | ApexBio | 2×500mL | EUR 78 |
Description: Hematoxylin and Eosin Staining,one of the most widely used staining technique in histopathology |
TRAP Staining Kit with Osteoplates |
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KT-651 | Kamiya Biomedical Company | 1 kit | EUR 1003 |
7-AAD Viability Staining Solution |
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EXB0026 | ExBio | 400 tests | EUR 91.2 |
Simport EasyDip Staining Jar Yellow |
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HIS0277 | Scientific Laboratory Supplies | PK6 | EUR 110.4 |
SafePinky DNA Gel Staining Solution |
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S1000-050 | GenDepot | 500ml | EUR 157.2 |
SafePinky DNA Gel Staining Solution |
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S1000-100 | GenDepot | 2X500ml | EUR 225.6 |
SafePinky DNA Gel Staining Solution |
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S1000-200 | GenDepot | 4X500ml | EUR 336 |
7-AAD Viability Staining Solution |
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E16FXP003 | EnoGene | 150 tests | EUR 40 |
Description: Available in various conjugation types. |
Hoechst 33342 / PI Double Staining Kit |
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abx097209-1Kit | Abbexa | 1 Kit | EUR 292.8 |
CaspGLOW Active Caspase Staining Kit |
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55R-1292 | Fitzgerald | 25 assays | EUR 355.2 |
Description: Caspase Staining Kit for use activity in the research laboratory |
Mouse to Mouse HRP Staining System |
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MTM003 | ScyTek Laboratories | 400 Slides | EUR 433.2 |
Coomassie Blue Fast Staining Solution |
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AR0170 | BosterBio | 100mL(for 10 assays to stain gels of 5 x 8.5cm) | EUR 96 |
Staining Jar Schiefferdecker With Lid |
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MIC6014 | Scientific Laboratory Supplies | EACH | EUR 22.8 |
Alkaline Phosphatase Red Staining kit |
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TBS2080 | Tribioscience | 50 tests | EUR 89 |
Human Type I Collagen Staining Kit |
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9044 | Chondrex | 1 kit | EUR 406.26 |
Description: Human Type I Collagen Staining Kit |
Red Active Caspase-3 Staining Kit |
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K2053-100 | ApexBio | 100 assays | EUR 686.4 |
Red Active Caspase-3 Staining Kit |
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K2053-25 | ApexBio | 25 assays | EUR 285.6 |
Red Active Caspase-8 Staining Kit |
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K2054-100 | ApexBio | 100 assays | EUR 686.4 |
Red Active Caspase-8 Staining Kit |
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K2054-25 | ApexBio | 25 assays | EUR 285.6 |
Red Active Caspase-9 Staining Kit |
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K2055-100 | ApexBio | 100 assays | EUR 686.4 |
Red Active Caspase-9 Staining Kit |
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K2055-25 | ApexBio | 25 assays | EUR 285.6 |
Calcein-AM/PI Double Staining Kit |
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E-CK-A354-100Assays | Elabscience Biotech | 100 Assays | EUR 100 |
Calcein-AM/PI Double Staining Kit |
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E-CK-A354-2000Assays | Elabscience Biotech | 2000 Assays | EUR 600 |
Calcein-AM/PI Double Staining Kit |
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E-CK-A354-500Assays | Elabscience Biotech | 500 Assays | EUR 300 |
Stayright™ Purple HRP Staining Kit |
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45905 | AAT Bioquest | 5 mL | EUR 109 |
Stayright™ Purple HRP Staining Kit |
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45906 | AAT Bioquest | 50 mL | EUR 334 |
OOSB00015-500ML - Cell Staining Buffer |
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OOSB00015-500ML | Aviva Systems Biology | 500mL | EUR 119 |
OOSB00016-250ML - Cell Staining Buffer |
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OOSB00016-250ML | Aviva Systems Biology | 250mL | EUR 69 |
OOSB00017-100ML - Cell Staining Buffer |
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OOSB00017-100ML | Aviva Systems Biology | 100mL | EUR 39 |
OOTA00782-500ML - Flow Staining Buffer |
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OOTA00782-500ML | Aviva Systems Biology | 500mL | EUR 79 |
Alkaline Phosphatase Blue Staining kit |
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TBS2085 | Tribioscience | 50 tests | EUR 89 |
Hellandahl Staining Jar+Lid 16 slide |
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MIC6004 | Scientific Laboratory Supplies | EACH | EUR 34.8 |
EasyDip Slide Staining Jar - Blue, each |
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M900-12B | IHC World | - | EUR 16.98 |
EasyDip Slide Staining Jar - Pink, each |
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M900-12P | IHC World | - | EUR 16.98 |
Four color suit nuclear staining Kit |
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EGY071 | EnoGene | 100T | EUR 1190 |
Hoechst 33342 staining kit (ready to use) |
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EGY0631 | EnoGene | 10mL | EUR 50 |
Hoechst 33342 staining kit (ready to use) |
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EGY0632 | EnoGene | 50mL | EUR 160 |
Hoechst 33258 staining kit (ready to use) |
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EGY0641 | EnoGene | 10mL | EUR 50 |
Hoechst 33258 staining kit (ready to use) |
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EGY0642 | EnoGene | 50mL | EUR 145 |
Phosphorylated protein gel fluorescent staining kit |
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E1WP329 | EnoGene | 250mL / 5 gels | EUR 300 |
Fluorescein Active Caspase Staining Kit |
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K2047-100 | ApexBio | 100 assays | EUR 686.4 |
Fluorescein Active Caspase Staining Kit |
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K2047-25 | ApexBio | 25 assays | EUR 285.6 |
Foxp3/Transcription Factor Staining Kit |
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E-CK-A108-20Assays | Elabscience Biotech | 20 Assays | EUR 75 |
Coplin Staining Jar Glass+Lid 10Slide |
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MIC6000 | Scientific Laboratory Supplies | EACH | EUR 13.2 |
Yeast Viability Staining Kit (Red/Blue) |
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31063-2-1 | Biotium | KT | EUR 475 |
EasyDip Slide Staining Jar - Green, each |
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M900-12G | IHC World | - | EUR 16.98 |
EasyDip Slide Staining Jar - White, each |
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M900-12W | IHC World | - | EUR 16.98 |
EasyDip Slide Staining Rack - Gray, each |
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M905-12DGY | IHC World | - | EUR 17.98 |
Prosi Silver Staining Kit for 25 mini gel |
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S3200-020 | GenDepot | 1kit | EUR 433.2 |
Propidium iodide staining kit (ready to use) |
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EGY0651 | EnoGene | 5mL | EUR 90 |
Propidium iodide staining kit (ready to use) |
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EGY0652 | EnoGene | 10mL | EUR 125 |
Propidium iodide staining kit (ready to use) |
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EGY0653 | EnoGene | 20mL | EUR 240 |
BRAND(TM) STAINING TRAY FOR 25 SLIDES |
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BR471400-5EA | Scientific Laboratory Supplies | PK5 | EUR 58.1 |
BRAND(TM) STAINING TRAY FOR 20 SLIDES |
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BR474305-2EA | Scientific Laboratory Supplies | PK2 | EUR 43.2 |
Yeast Viability Staining Kit (Green/Red) |
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31063-1 | Biotium | 1kit | EUR 566.4 |
Description: Minimum order quantity: 1 unit of 1kit |
Gel-FAST? Gel Staining/Destaining Kit |
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K901-40 | Biovision | each | EUR 210 |
Safranin O Solution (For Gram Staining) |
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SOG125 | ScyTek Laboratories | 125 ml | EUR 26.57 |