The molecular mechanisms of pain associated with alphaherpesvirus latency is unclear. We hypothesize that the voltage-gated sodium channels (VGSC) in dorsal root ganglion (DRG) neurons controlling the electrical impulses may have abnormal activity during infection and reactivation of latent virus. We used HSV-1 infects DRG-derived neural cell line of human HD10.6 to study the formation of viral latency, maintenance and reactivation and changes of VGSC functional expression. differentiated cells exhibited strong tetrodotoxin (TTX) sensitive sodium currents and significantly reduce the acute infection VGSC functional expression within 24 hours, and completely eliminate VGSC activity within 3 days.
A stationary state of infection mimic latency can be achieved in the presence acyclovir (ACV) for 7 days followed by 5 days of washout ACV and then the virus can remain active for three weeks. It was noted that during the formation of HSV-1 latency, loss VGSC activity caused by HSV-1 can not be blocked by the treatment of ACV. However, neurons with ACV treatment continued for 4 days showed a gradual recovery VGSC functional expression.
Furthermore, neurons VGSC latent showed higher activity compared to controls. Overall Regulation VGSC by HSV-1 during a silent infection as evidenced by an increase in the transcription and translation possibilities Nav1.7. Together these observations suggest a very complex pattern of electrophysiological changes during HSV infection DRG neurons, which may have implications for understanding the mechanisms of virus-mediated pain associated with latency and reactivation.Importance: The reactivation of the herpes virus, most often VZV and PRV, can cause nerve disorders cranial and unbearable pain. Clinical studies have also reported that HSV-1 causes occipital neuralgia and chronic postherpetic neuralgia in humans.
Work is currently closely studying the functional VGSC expression profile changes during the process of HSV-1 latency and reactivation formation using human dorsal root ganglion neurons HD 10.6 cells as an in vitro model. Our results show that the activity of VGSC eliminated after infection but continued to recover during the establishment of latency and latency neurons showed VGSC activity is even higher. This finding advances our knowledge of how the ganglion neurons generated electrical impulses as usual for normal VGSC functional expression is affected by latent viruses.
2X PCR HotStart MasterMix with dye |
G906-dye |
ABM |
5.0 ml (200 Rxns) |
EUR 189.6 |
2X PCR Taq Plus MasterMix with dye |
G014-dye |
ABM |
5.0 ml (200 Rxns) |
EUR 127.2 |
2X PCR Precision™ MasterMix with dye |
G124-dye |
ABM |
5.0 ml (200 Rxns) |
EUR 195.6 |
One-Step RT-PCR Kit with dye |
G174-dye |
ABM |
100 x 50 ul reactions |
EUR 279.6 |
2X PCR Bestaq™ MasterMix with dye |
G464-dye |
ABM |
5.0 ml (200 Rxns) |
EUR 189.6 |
2X PCR Taq Plus MasterMix with dye |
G901-dye |
ABM |
25.0 ml (1000 Rxns) |
EUR 358.8 |
2X PCR SensTaq HotStart MasterMix with Dye |
G939-Dye |
ABM |
5.0 ml (200 Rxns) |
EUR 189.6 |
DAPI |
abx082063-10mg |
Abbexa |
10 mg |
EUR 276 |
|
DAPI (hydrochloride) |
C3362-10 |
ApexBio |
10 mg |
EUR 146.4 |
DAPI (hydrochloride) |
C3362-25 |
ApexBio |
25 mg |
EUR 240 |
DAPI (hydrochloride) |
C3362-5 |
ApexBio |
5 mg |
EUR 111.6 |
DAPI Staining Kit |
abx097206-1Kit |
Abbexa |
1 Kit |
EUR 276 |
|
FluorLast? with DAPI |
1214-20 |
Biovision |
each |
EUR 183.6 |
DAPI (Molecular Biology Grade) |
CE117 |
GeneOn |
5 mg |
EUR 72 |
DAPI (Molecular Biology Grade) |
CE118 |
GeneOn |
25 mg |
EUR 159.6 |
DAPI (Molecular Biology Grade) |
CE119 |
GeneOn |
100 mg |
EUR 382.8 |
Phycocyanin dye |
85R-1016 |
Fitzgerald |
1 mg |
EUR 159.6 |
Description: Phycocyanin dye for use in conjugation to biomolecules and antibodies |
Phycoerythrin dye |
85R-1018 |
Fitzgerald |
1 mg |
EUR 159.6 |
Description: Phycocyanin dye for use in conjugation to biomolecules and antibodies |
dye Antibody |
1-CSB-PA772049LA01DIL |
Cusabio |
|
|
|
Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:5000 |
FluorLast? with DAPI and DABCO |
1218-20 |
Biovision |
each |
EUR 183.6 |
FluoroQuest™ Mounting Medium with DAPI |
20004 |
AAT Bioquest |
50 mL |
EUR 158.4 |
|
EverBrite Hardset Mounting Medium with DAPI |
23004 |
Biotium |
10mL |
EUR 255.6 |
Description: Minimum order quantity: 1 unit of 10mL |
Anti-Fade Mounting Medium (With DAPI) |
abx090690-10ml |
Abbexa |
10 ml |
EUR 243.6 |
|
Bradford Dye Reagent |
0209R |
AthenaES |
100 ml |
EUR 157.2 |
PCR SuperMix (-dye) |
20-abx098002 |
Abbexa |
-
EUR 493.20
-
EUR 226.80
-
EUR 309.60
|
|
|
PCR SuperMix (+dye) |
20-abx098003 |
Abbexa |
-
EUR 493.20
-
EUR 226.80
-
EUR 7408.80
-
EUR 309.60
|
|
|
DNA Loading Dye |
G030 |
ABM |
6X; 3 x 1.0 ml |
EUR 94.8 |
Green-DNA Dye |
SB2040 |
Bio Basic |
100UL, 100UL |
EUR 80.88 |
|
Liposomal Fluorescein Dye |
DPC001FL |
ProFoldin |
1 ml |
EUR 140.62 |
Description: This product includes 1 ml of 0.1 mM Fluorescein encapsulated in liposome composed of DPPC:Cholesterol (w/w 1:1). |
DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) |
B551 |
ABM |
0.2 ml |
EUR 129.6 |
ROX passive reference dye |
29052 |
Biotium |
5mL |
EUR 234 |
Description: Minimum order quantity: 1 unit of 5mL |
T PCR SuperMix (-dye) |
20-abx098005 |
Abbexa |
|
|
|
T PCR SuperMix (+dye) |
20-abx098006 |
Abbexa |
|
|
|
EasyPfu PCR SuperMix (-dye) |
20-abx098009 |
Abbexa |
|
|
|
FastPfu PCR SuperMix (-dye) |
20-abx098010 |
Abbexa |
|
|
|
Phycocyanin dye (cross-linked) |
85R-1017 |
Fitzgerald |
1 mg |
EUR 146.4 |
Description: Cross-linked phycocyanin dye for use in conjugation to biomolecules and antibodies |
dye Antibody, HRP conjugated |
1-CSB-PA772049LB01DIL |
Cusabio |
|
|
|
Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is HRP conjugated. Tested in the following application: ELISA |
dye Antibody, FITC conjugated |
1-CSB-PA772049LC01DIL |
Cusabio |
|
|
|
Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is FITC conjugated. Tested in the following application: ELISA |
dye Antibody, Biotin conjugated |
1-CSB-PA772049LD01DIL |
Cusabio |
|
|
|
Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is Biotin conjugated. Tested in the following application: ELISA |
DNA Loading Green Dye |
G924 |
ABM |
6X; 3 x 1.0 ml |
EUR 94.8 |
INT dye (Iodonitrotetrazolium chloride) |
IB0280 |
Bio Basic |
250mg |
EUR 75.66 |
|
Fluorescein Reference Dye (100X) |
F553 |
GeneOn |
1 ml (10µM) |
EUR 73.2 |
Fluorescein Reference Dye (100X) |
F553L |
GeneOn |
5x1 ml (50µM) |
EUR 206.4 |
Rox Passive Reference Dye |
R1000-500 |
GenDepot |
500ul |
EUR 138 |
Liposomal Acridine Orange Dye |
DPC001AO |
ProFoldin |
1 ml |
EUR 140.62 |
Description: This product includes 1 ml of 0.1 mM acridine orange encapsulated in liposome composed of DPPC:Cholesterol (w/w 1:1). |
Liposomal Rhodamine G Dye |
DPC001RG |
ProFoldin |
1 ml |
EUR 140.62 |
Description: This product includes 1 ml of 0.1 mM Rhodamine G encapsulated in liposome composed of DPPC:Cholesterol (w/w 1:1). |
Ready? PCR Mix-Dye |
M1128-1000 |
Biovision |
each |
EUR 614.4 |
Ready? PCR Mix-Dye |
M1128-200 |
Biovision |
each |
EUR 229.2 |
Rigor? PCR Mix-Dye |
M1133-200 |
Biovision |
each |
EUR 385.2 |
Breeze? PCR Mix-Dye |
M1135-200 |
Biovision |
each |
EUR 320.4 |
Distant? PCR Mix-Dye |
M1137-200 |
Biovision |
each |
EUR 352.8 |
Advance? PCR Mix-Dye |
M1140-200 |
Biovision |
each |
EUR 451.2 |
FluoroQuest™ Anti-fading Mounting Medium with DAPI |
20005 |
AAT Bioquest |
20 mL |
EUR 211 |
PCR SuperMix for PAGE (+dye) |
20-abx098004 |
Abbexa |
-
EUR 493.20
-
EUR 226.80
-
EUR 309.60
|
|
|
BCIP-NBT Dye for IHC |
G447 |
ABM |
250 ml |
EUR 304.8 |
G50 Dye Terminator Removal Kit |
FYG603-50P |
Yeastern Biotech |
50 Preps |
Ask for price |
iVDye 6X Gel Loading Dye |
V1004-001 |
GenDepot |
1ml |
EUR 87.6 |
iVDye 6X Gel Loading Dye |
V1004-010 |
GenDepot |
10x1ml |
EUR 213.6 |
iVDye 6X Gel Loading Dye |
V1004-020 |
GenDepot |
20x1ml |
EUR 258 |
Powerpol 2xPCR mix with dye |
RK20719 |
Abclonal |
1mL |
Ask for price |
|
PEGylated Liposomal Acridine Orange Dye |
PHC001AO |
ProFoldin |
1 ml |
EUR 140.62 |
Description: This product includes 1 ml of 0.1 mM acridine orange encapsulated in liposome composed of HSPC:DSPE-PEG2000:Cholesterol (w/w 3:1:1). |
PEGylated Liposomal Rhodamine B Dye |
PHC001RB |
ProFoldin |
1 ml |
EUR 140.62 |
Description: This product includes 1 ml of 0.1 mM Rhodamine B encapsulated in liposome composed of HSPC:DSPE-PEG2000:Cholesterol (w/w 3:1:1). |
Robust Ready? PCR Mix-Dye |
M1131-1000 |
Biovision |
each |
EUR 738 |
Robust Ready? PCR Mix-Dye |
M1131-200 |
Biovision |
each |
EUR 255.6 |
Fire Start? PCR Mix-Dye |
M1142-200 |
Biovision |
each |
EUR 451.2 |
Top Green qPCR SuperMix (+Dye II) |
20-abx098890 |
Abbexa |
-
EUR 994.80
-
EUR 260.40
-
EUR 1362.00
-
EUR 493.20
|
|
|
Tip Green qPCR SuperMix (+Dye II) |
20-abx098891 |
Abbexa |
-
EUR 1161.60
-
EUR 276.00
-
EUR 1596.00
-
EUR 560.40
|
|
|
Load 6x Loading Dye Bromophenol Blue |
BA01401 |
Bioatlas |
6x1ml |
EUR 96 |
Description: High purity buffer for various PCR applications. |
Load 6x Loading Dye Orange G |
BA01402 |
Bioatlas |
6x1ml |
EUR 96 |
Description: High purity buffer for various PCR applications. |
Green-DNA Dye, ready to use |
DT81414 |
Bio Basic |
1.5ml, 1.5ml |
EUR 153.96 |
|
TaqProbe 5X qPCR MasterMix-No Dye |
MasterMix-5PS |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 255.6 |
BrightGreen 5X qPCR MasterMix-No Dye |
MasterMix-5S |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 255.6 |
KiloGreen 2X qPCR MasterMix-No Dye |
MasterMix-KS |
ABM |
4 x 1.25 ml - 500 reactions (20 ul) |
EUR 168 |
BrightGreen miRNA qPCR MasterMix-No Dye |
MasterMix-mS |
ABM |
4 x 1.25 ml for 500 reactions |
EUR 181.2 |
TaqProbe 2X qPCR MasterMix-No Dye |
MasterMix-PS |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 168 |
BrightGreen 2X qPCR MasterMix-No Dye |
MasterMix-S |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 168 |
BrightGreen 2X qPCR MasterMix-No Dye |
MasterMix-S-XL |
ABM |
16 x 1.25 ml for 2000 reactions (20 ul) |
EUR 451.2 |
2 × Taq Master Mix (Dye Plus) |
P112-01 |
Vazyme |
5 ml |
EUR 140.4 |
2 × Taq Master Mix (Dye Plus) |
P112-02 |
Vazyme |
15 ml |
EUR 175.2 |
2 × Taq Master Mix (Dye Plus) |
P112-03 |
Vazyme |
50 ml |
EUR 283.2 |
Fast Red Violet Dye for IHC |
G446 |
ABM |
250 ml |
EUR 410.4 |
ROX Reference Dye (25 µM; 50x) |
F153 |
GeneOn |
1 ml |
EUR 73.2 |
ROX Reference Dye (25 µM; 50x) |
F153L |
GeneOn |
5x1 ml |
EUR 206.4 |
2 × AceTaq Master Mix (Dye Plus) |
P412-01 |
Vazyme |
1 ml |
EUR 142.8 |
2 × AceTaq Master Mix (Dye Plus) |
P412-02 |
Vazyme |
5 ml |
EUR 189.6 |
2 × AceTaq Master Mix (Dye Plus) |
P412-03 |
Vazyme |
15 ml |
EUR 314.4 |
Easy? One Step RT Kit-Dye |
M1163-100 |
Biovision |
each |
EUR 561.6 |
VIC Dye qPCR Calibration Solution *10,000X* |
67030 |
AAT Bioquest |
100 ul |
EUR 301 |
NED Dye qPCR Calibration Solution *10,000X* |
67031 |
AAT Bioquest |
100 ul |
EUR 301 |
DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3* |
17510 |
AAT Bioquest |
10 mg |
EUR 138 |
|
DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3* |
17511 |
AAT Bioquest |
100 mg |
EUR 262.8 |
|
DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3* |
17513 |
AAT Bioquest |
25 mg |
EUR 158.4 |
|
Human 4’,6-Diamidino-2-phenylindole dihydrochloride(DAPI)ELISA kit |
QY-E05538 |
Qayee Biotechnology |
96T |
EUR 433.2 |
Yeast Live-or-Dye Fixable Live/Dead Staining Kit (TO and Live-or-Dye 568/583) |
31064 |
Biotium |
1kit |
EUR 526.8 |
Description: Minimum order quantity: 1 unit of 1kit |
Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye |
MB1004-100Reactions |
BosterBio |
100 Reactions |
EUR 392.4 |
Cyanine2 NHS ester minimal dye, 25 nmol |
3A041 |
Lumiprobe |
25 nmol |
EUR 218.4 |
Cyanine3 NHS ester minimal dye, 25 nmol |
3B041 |
Lumiprobe |
25 nmol |
EUR 218.4 |
Cyanine5 NHS ester minimal dye, 25 nmol |
3C041 |
Lumiprobe |
25 nmol |
EUR 218.4 |
1000bp/1kb DNA ladder (no loading dye) |
305-005 |
GeneOn |
50µg/250µl |
EUR 60 |
1000bp/1kb DNA ladder (no loading dye) |
305-025 |
GeneOn |
5x50µg |
EUR 144 |
GelRed Prestain Plus 6X DNA Loading Dye |
41011 |
Biotium |
1mL |
EUR 176.4 |
Description: Minimum order quantity: 1 unit of 1mL |
ROX reference dye for qPCR, 1 mL |
41110 |
Lumiprobe |
1 mL |
EUR 62.4 |
FALCON® CALCEIN AM FLUORESCENT DYE, 1MG |
354217 |
CORNING |
1/pk |
EUR 362.4 |
Description: Permeable Support - DL; Coated Transwells - DL |
FALCON® DIIC12(3) FLUORESCENT DYE, 100MG |
354218 |
CORNING |
1/pk |
EUR 381.6 |
Description: Permeable Support - DL; Coated Transwells - DL |
Cyanine2 NHS ester minimal dye, 5 nmol |
1A041 |
Lumiprobe |
5 nmol |
EUR 111.6 |
Cyanine3 NHS ester minimal dye, 5 nmol |
1B041 |
Lumiprobe |
5 nmol |
EUR 111.6 |
Cyanine5 NHS ester minimal dye, 5 nmol |
1C041 |
Lumiprobe |
5 nmol |
EUR 111.6 |
Cyanine2 NHS ester minimal dye, 10 nmol |
2A041 |
Lumiprobe |
10 nmol |
EUR 156 |
Cyanine3 NHS ester minimal dye, 10 nmol |
2B041 |
Lumiprobe |
10 nmol |
EUR 152.4 |
Cyanine5 NHS ester minimal dye, 10 nmol |
2C041 |
Lumiprobe |
10 nmol |
EUR 152.4 |
HotStart PCR Master mix (2X, Green Dye) |
BS9291 |
Bio Basic |
5X1mL, 5ml |
EUR 251.4 |
|
HotStart PCR Master mix (2X, Green Dye) |
BS9292 |
Bio Basic |
1ml |
EUR 115.68 |
|
Taq PCR Master Mix (2X, Blue Dye) |
BS9295 |
Bio Basic |
1ml |
EUR 101.76 |
|
Taq PCR Master Mix (2X, Blue Dye) |
BS9296 |
Bio Basic |
5ml |
EUR 227.04 |
|
Taq PCR Master Mix (2X, Red Dye) |
BS9297 |
Bio Basic |
1ml |
EUR 101.76 |
|
Taq PCR Master Mix (2X, Red Dye) |
BS9298 |
Bio Basic |
5ml |
EUR 227.04 |
|
Eco-Red-DNA Dye, ready to use |
DT81415 |
Bio Basic |
1ml |
EUR 122.64 |
|
Eco-White-DNA Dye, ready to use |
DT81417 |
Bio Basic |
1ml |
EUR 122.64 |
|
BrightGreen Express 2X qPCR MasterMix-No Dye |
MasterMix-ES |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 168 |
One-Step BrightGreen qRT-PCR-No Dye |
G471-S |
ABM |
100 rxn (20 ul/rxn) |
EUR 189.6 |
One-Step TaqProbe qRT-PCR-No Dye |
G493-PS |
ABM |
100 rxn (20 ul/rxn) |
EUR 189.6 |
2×Taq PCR Master Mix(with dye) |
K1034-1 |
ApexBio |
1 ml |
EUR 87.6 |
2×Taq PCR Master Mix(with dye) |
K1034-100 |
ApexBio |
100×1 ml |
EUR 950.4 |
2×Taq PCR Master Mix(with dye) |
K1034-20 |
ApexBio |
20×1 ml |
EUR 338.4 |
2×Taq PCR Master Mix(with dye) |
K1034-5 |
ApexBio |
5×1 ml |
EUR 142.8 |
2×Taq PCR Master Mix(with dye) |
K1034-50 |
ApexBio |
50×1 ml |
EUR 616.8 |
2 × Taq Plus Master Mix (Dye Plus) |
P212-01 |
Vazyme |
5 ml |
EUR 159.6 |
2 × Taq Plus Master Mix (Dye Plus) |
P212-02 |
Vazyme |
15 ml |
EUR 231.6 |
2 × Taq Plus Master Mix (Dye Plus) |
P212-03 |
Vazyme |
50 ml |
EUR 466.8 |