Microporator

Very low volume electroporation devices for Jurkats and DRG neurons.

Modulation of Voltage-Gated Sodium Channel (VGSC) Activity in Human Dorsal Root Ganglion (DRG) Neurons by Herpesvirus Quiescent Infection.

The molecular mechanisms of pain associated with alphaherpesvirus latency is unclear. We hypothesize that the voltage-gated sodium channels (VGSC) in dorsal root ganglion (DRG) neurons controlling the electrical impulses may have abnormal activity during infection and reactivation of latent virus. We used HSV-1 infects DRG-derived neural cell line of human HD10.6 to study the formation of viral latency, maintenance and reactivation and changes of VGSC functional expression. differentiated cells exhibited strong tetrodotoxin (TTX) sensitive sodium currents and significantly reduce the acute infection VGSC functional expression within 24 hours, and completely eliminate VGSC activity within 3 days.

A stationary state of infection mimic latency can be achieved in the presence acyclovir (ACV) for 7 days followed by 5 days of washout ACV and then the virus can remain active for three weeks. It was noted that during the formation of HSV-1 latency, loss VGSC activity caused by HSV-1 can not be blocked by the treatment of ACV. However, neurons with ACV treatment continued for 4 days showed a gradual recovery VGSC functional expression.

Furthermore, neurons VGSC latent showed higher activity compared to controls. Overall Regulation VGSC by HSV-1 during a silent infection as evidenced by an increase in the transcription and translation possibilities Nav1.7. Together these observations suggest a very complex pattern of electrophysiological changes during HSV infection DRG neurons, which may have implications for understanding the mechanisms of virus-mediated pain associated with latency and reactivation.Importance: The reactivation of the herpes virus, most often VZV and PRV, can cause nerve disorders cranial and unbearable pain. Clinical studies have also reported that HSV-1 causes occipital neuralgia and chronic postherpetic neuralgia in humans.

Work is currently closely studying the functional VGSC expression profile changes during the process of HSV-1 latency and reactivation formation using human dorsal root ganglion neurons HD 10.6 cells as an in vitro model. Our results show that the activity of VGSC eliminated after infection but continued to recover during the establishment of latency and latency neurons showed VGSC activity is even higher. This finding advances our knowledge of how the ganglion neurons generated electrical impulses as usual for normal VGSC functional expression is affected by latent viruses.

2X PCR HotStart MasterMix with dye
G906-dye 5.0 ml (200 Rxns)
EUR 189.6

2X PCR Taq Plus MasterMix with dye
G014-dye 5.0 ml (200 Rxns)
EUR 127.2

2X PCR Precision™ MasterMix with dye
G124-dye 5.0 ml (200 Rxns)
EUR 195.6

One-Step RT-PCR Kit with dye
G174-dye 100 x 50 ul reactions
EUR 279.6

2X PCR Bestaq™ MasterMix with dye
G464-dye 5.0 ml (200 Rxns)
EUR 189.6

2X PCR Taq Plus MasterMix with dye
G901-dye 25.0 ml (1000 Rxns)
EUR 358.8

2X PCR SensTaq HotStart MasterMix with Dye
G939-Dye 5.0 ml (200 Rxns)
EUR 189.6

DAPI
abx082063-10mg 10 mg
EUR 276

DAPI
B1098-25 each
EUR 496.8

DAPI
B1098-5 each
EUR 170.4

DAPI Dilactate
17509 25 mg
EUR 158.4

DAPI (hydrochloride)
C3362-10 10 mg
EUR 146.4

DAPI (hydrochloride)
C3362-25 25 mg
EUR 240

DAPI (hydrochloride)
C3362-5 5 mg
EUR 111.6

DAPI (dihydrochloride)
HY-D0814 50mg
EUR 439.2

DAPI Staining Kit
abx097206-1Kit 1 Kit
EUR 276

FluorLast? with DAPI
1214-20 each
EUR 183.6

DAPI (Molecular Biology Grade)
CE117 5 mg
EUR 72

DAPI (Molecular Biology Grade)
CE118 25 mg
EUR 159.6

DAPI (Molecular Biology Grade)
CE119 100 mg
EUR 382.8

Phycocyanin dye
85R-1016 1 mg
EUR 159.6
Description: Phycocyanin dye for use in conjugation to biomolecules and antibodies

Phycoerythrin dye
85R-1018 1 mg
EUR 159.6
Description: Phycocyanin dye for use in conjugation to biomolecules and antibodies

dye Antibody
1-CSB-PA772049LA01DIL
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:5000

Dye 937
HY-15422 5mg
EUR 514.8

Dye 993
HY-15423 5mg
EUR 514.8

D149 Dye
HY-50938 50mg
EUR 325.2

FluorLast? with DAPI and DABCO
1218-20 each
EUR 183.6

FluoroQuest™ Mounting Medium with DAPI
20004 50 mL
EUR 158.4

EverBrite Hardset Mounting Medium with DAPI
23004 10mL
EUR 255.6
Description: Minimum order quantity: 1 unit of 10mL

Anti-Fade Mounting Medium (With DAPI)
abx090690-10ml 10 ml
EUR 243.6

Bradford Dye Reagent
0209R 100 ml
EUR 157.2

PCR SuperMix (-dye)
20-abx098002
  • EUR 493.20
  • EUR 226.80
  • EUR 309.60
  • 15 ml
  • 1 ml
  • 5 ml

PCR SuperMix (+dye)
20-abx098003
  • EUR 493.20
  • EUR 226.80
  • EUR 7408.80
  • EUR 309.60
  • 15 ml
  • 1 ml
  • 480 ml
  • 5 ml

DNA Loading Dye
G030 6X; 3 x 1.0 ml
EUR 94.8

Green-DNA Dye
SB2040 100UL, 100UL
EUR 80.88

Dye Solution A
IRDSA01 100ML
EUR 188.1

Dye removal bag
DD4905007 EACH
EUR 224.58

Liposomal Fluorescein Dye
DPC001FL 1 ml
EUR 140.62
Description: This product includes 1 ml of 0.1 mM Fluorescein encapsulated in liposome composed of DPPC:Cholesterol (w/w 1:1).

DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)
B551 0.2 ml
EUR 129.6

ROX passive reference dye
29052 5mL
EUR 234
Description: Minimum order quantity: 1 unit of 5mL

T PCR SuperMix (-dye)
20-abx098005
  • EUR 243.60
  • EUR 410.40
  • 1 ml
  • 5 ml

T PCR SuperMix (+dye)
20-abx098006
  • EUR 243.60
  • EUR 410.40
  • 1 ml
  • 5 ml

EasyPfu PCR SuperMix (-dye)
20-abx098009
  • EUR 243.60
  • EUR 410.40
  • 1 ml
  • 5 ml

FastPfu PCR SuperMix (-dye)
20-abx098010
  • EUR 292.80
  • EUR 594.00
  • 1 ml
  • 5 ml

Phycocyanin dye (cross-linked)
85R-1017 1 mg
EUR 146.4
Description: Cross-linked phycocyanin dye for use in conjugation to biomolecules and antibodies

dye Antibody, HRP conjugated
1-CSB-PA772049LB01DIL
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is HRP conjugated. Tested in the following application: ELISA

dye Antibody, FITC conjugated
1-CSB-PA772049LC01DIL
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is FITC conjugated. Tested in the following application: ELISA

dye Antibody, Biotin conjugated
1-CSB-PA772049LD01DIL
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is Biotin conjugated. Tested in the following application: ELISA

DNA Loading Green Dye
G924 6X; 3 x 1.0 ml
EUR 94.8

Gel Loading Dye Solution
FYD003-3ML 3 ml Ask for price

INT dye (Iodonitrotetrazolium chloride)
IB0280 250mg
EUR 75.66

Heptamethine cyanine dye-1
HY-D0921 50mg
EUR 2539.2

Fluorescein Reference Dye (100X)
F553 1 ml (10µM)
EUR 73.2

Fluorescein Reference Dye (100X)
F553L 5x1 ml (50µM)
EUR 206.4

Rox Passive Reference Dye
R1000-500 500ul
EUR 138

Cye Dye Bulk Pack
PA55322 EACH
EUR 3335.64

Liposomal Acridine Orange Dye
DPC001AO 1 ml
EUR 140.62
Description: This product includes 1 ml of 0.1 mM acridine orange encapsulated in liposome composed of DPPC:Cholesterol (w/w 1:1).

Liposomal Rhodamine G Dye
DPC001RG 1 ml
EUR 140.62
Description: This product includes 1 ml of 0.1 mM Rhodamine G encapsulated in liposome composed of DPPC:Cholesterol (w/w 1:1).

Ready? PCR Mix-Dye
M1128-1000 each
EUR 614.4

Ready? PCR Mix-Dye
M1128-200 each
EUR 229.2

Rigor? PCR Mix-Dye
M1133-200 each
EUR 385.2

Breeze? PCR Mix-Dye
M1135-200 each
EUR 320.4

Distant? PCR Mix-Dye
M1137-200 each
EUR 352.8

Advance? PCR Mix-Dye
M1140-200 each
EUR 451.2

FluoroQuest™ Anti-fading Mounting Medium with DAPI
20005 20 mL
EUR 211

PCR SuperMix for PAGE (+dye)
20-abx098004
  • EUR 493.20
  • EUR 226.80
  • EUR 309.60
  • 15 ml
  • 1 ml
  • 5 ml

BCIP-NBT Dye for IHC
G447 250 ml
EUR 304.8

G50 Dye Terminator Removal Kit
FYG603-50P 50 Preps Ask for price

iVDye 6X Gel Loading Dye
V1004-001 1ml
EUR 87.6

iVDye 6X Gel Loading Dye
V1004-010 10x1ml
EUR 213.6

iVDye 6X Gel Loading Dye
V1004-020 20x1ml
EUR 258

Powerpol 2xPCR mix with dye
RK20719 1mL Ask for price

PEGylated Liposomal Acridine Orange Dye
PHC001AO 1 ml
EUR 140.62
Description: This product includes 1 ml of 0.1 mM acridine orange encapsulated in liposome composed of HSPC:DSPE-PEG2000:Cholesterol (w/w 3:1:1).

PEGylated Liposomal Rhodamine B Dye
PHC001RB 1 ml
EUR 140.62
Description: This product includes 1 ml of 0.1 mM Rhodamine B encapsulated in liposome composed of HSPC:DSPE-PEG2000:Cholesterol (w/w 3:1:1).

Robust Ready? PCR Mix-Dye
M1131-1000 each
EUR 738

Robust Ready? PCR Mix-Dye
M1131-200 each
EUR 255.6

Fire Start? PCR Mix-Dye
M1142-200 each
EUR 451.2

Buccutite™ MTA-Dye 650
5370 2 umoles
EUR 222

Buccutite™ FOL-Dye 650
5372 2 umoles
EUR 222

Top Green qPCR SuperMix (+Dye II)
20-abx098890
  • EUR 994.80
  • EUR 260.40
  • EUR 1362.00
  • EUR 493.20
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Tip Green qPCR SuperMix (+Dye II)
20-abx098891
  • EUR 1161.60
  • EUR 276.00
  • EUR 1596.00
  • EUR 560.40
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Load 6x Loading Dye Bromophenol Blue
BA01401 6x1ml
EUR 96
Description: High purity buffer for various PCR applications.

Load 6x Loading Dye Orange G
BA01402 6x1ml
EUR 96
Description: High purity buffer for various PCR applications.

Green-DNA Dye, ready to use
DT81414 1.5ml, 1.5ml
EUR 153.96

TaqProbe 5X qPCR MasterMix-No Dye
MasterMix-5PS 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 255.6

BrightGreen 5X qPCR MasterMix-No Dye
MasterMix-5S 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 255.6

KiloGreen 2X qPCR MasterMix-No Dye
MasterMix-KS 4 x 1.25 ml - 500 reactions (20 ul)
EUR 168

BrightGreen miRNA qPCR MasterMix-No Dye
MasterMix-mS 4 x 1.25 ml for 500 reactions
EUR 181.2

TaqProbe 2X qPCR MasterMix-No Dye
MasterMix-PS 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

BrightGreen 2X qPCR MasterMix-No Dye
MasterMix-S 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

BrightGreen 2X qPCR MasterMix-No Dye
MasterMix-S-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 451.2

2 × Taq Master Mix (Dye Plus)
P112-01 5 ml
EUR 140.4

2 × Taq Master Mix (Dye Plus)
P112-02 15 ml
EUR 175.2

2 × Taq Master Mix (Dye Plus)
P112-03 50 ml
EUR 283.2

Fast Red Violet Dye for IHC
G446 250 ml
EUR 410.4

ROX Reference Dye (25 µM; 50x)
F153 1 ml
EUR 73.2

ROX Reference Dye (25 µM; 50x)
F153L 5x1 ml
EUR 206.4

2 × AceTaq Master Mix (Dye Plus)
P412-01 1 ml
EUR 142.8

2 × AceTaq Master Mix (Dye Plus)
P412-02 5 ml
EUR 189.6

2 × AceTaq Master Mix (Dye Plus)
P412-03 15 ml
EUR 314.4

Accuris Taq Master Mix Red Dye
PR1001-R-1000 1 PC
EUR 360.31

Accuris Taq Master Mix Red Dye
PR1001-R-200 1 PC
EUR 145.94

Accuris Taq Master Mix Red Dye
PR1001-R-S 1 PC
EUR 83.75

1.2ML Triple Dye Loading Buffer (6X)
NAT1192 1.2ML
EUR 29.75

Amersham Cy5 mono Reactive Dye Pack
PA25001 EACH
EUR 288.42

Easy? One Step RT Kit-Dye
M1163-100 each
EUR 561.6

VIC Dye qPCR Calibration Solution *10,000X*
67030 100 ul
EUR 301

NED Dye qPCR Calibration Solution *10,000X*
67031 100 ul
EUR 301

1X4 Color™ V405 Dye Set
8000 1 Set
EUR 1019

1X4 Color™ B488 Dye Set
8005 1 Set
EUR 1019

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*
17510 10 mg
EUR 138

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*
17511 100 mg
EUR 262.8

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*
17513 25 mg
EUR 158.4

Human 4’,6-Diamidino-2-phenylindole dihydrochloride（DAPI)ELISA kit
QY-E05538 96T
EUR 433.2

Yeast Live-or-Dye Fixable Live/Dead Staining Kit (TO and Live-or-Dye 568/583)
31064 1kit
EUR 526.8
Description: Minimum order quantity: 1 unit of 1kit

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye
MB1004-100Reactions 100 Reactions
EUR 392.4

Cyanine2 NHS ester minimal dye, 25 nmol
3A041 25 nmol
EUR 218.4

Cyanine3 NHS ester minimal dye, 25 nmol
3B041 25 nmol
EUR 218.4

Cyanine5 NHS ester minimal dye, 25 nmol
3C041 25 nmol
EUR 218.4

1000bp/1kb DNA ladder (no loading dye)
305-005 50µg/250µl
EUR 60

1000bp/1kb DNA ladder (no loading dye)
305-025 5x50µg
EUR 144

GelRed Prestain Plus 6X DNA Loading Dye
41011 1mL
EUR 176.4
Description: Minimum order quantity: 1 unit of 1mL

ROX reference dye for qPCR, 1 mL
41110 1 mL
EUR 62.4

FALCON® CALCEIN AM FLUORESCENT DYE, 1MG
354217 1/pk
EUR 362.4
Description: Permeable Support - DL; Coated Transwells - DL

FALCON® DIIC12(3) FLUORESCENT DYE, 100MG
354218 1/pk
EUR 381.6
Description: Permeable Support - DL; Coated Transwells - DL

Cyanine2 NHS ester minimal dye, 5 nmol
1A041 5 nmol
EUR 111.6

Cyanine3 NHS ester minimal dye, 5 nmol
1B041 5 nmol
EUR 111.6

Cyanine5 NHS ester minimal dye, 5 nmol
1C041 5 nmol
EUR 111.6

Cyanine2 NHS ester minimal dye, 10 nmol
2A041 10 nmol
EUR 156

Cyanine3 NHS ester minimal dye, 10 nmol
2B041 10 nmol
EUR 152.4

Cyanine5 NHS ester minimal dye, 10 nmol
2C041 10 nmol
EUR 152.4

HotStart PCR Master mix (2X, Green Dye)
BS9291 5X1mL, 5ml
EUR 251.4

HotStart PCR Master mix (2X, Green Dye)
BS9292 1ml
EUR 115.68

Taq PCR Master Mix (2X, Blue Dye)
BS9295 1ml
EUR 101.76

Taq PCR Master Mix (2X, Blue Dye)
BS9296 5ml
EUR 227.04

Taq PCR Master Mix (2X, Red Dye)
BS9297 1ml
EUR 101.76

Taq PCR Master Mix (2X, Red Dye)
BS9298 5ml
EUR 227.04

Eco-Red-DNA Dye, ready to use
DT81415 1ml
EUR 122.64

Eco-White-DNA Dye, ready to use
DT81417 1ml
EUR 122.64

BrightGreen Express 2X qPCR MasterMix-No Dye
MasterMix-ES 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

One-Step BrightGreen qRT-PCR-No Dye
G471-S 100 rxn (20 ul/rxn)
EUR 189.6

One-Step TaqProbe qRT-PCR-No Dye
G493-PS 100 rxn (20 ul/rxn)
EUR 189.6

2×Taq PCR Master Mix(with dye)
K1034-1 1 ml
EUR 87.6

2×Taq PCR Master Mix(with dye)
K1034-100 100×1 ml
EUR 950.4

2×Taq PCR Master Mix(with dye)
K1034-20 20×1 ml
EUR 338.4

2×Taq PCR Master Mix(with dye)
K1034-5 5×1 ml
EUR 142.8

2×Taq PCR Master Mix(with dye)
K1034-50 50×1 ml
EUR 616.8

2 × Taq Plus Master Mix (Dye Plus)
P212-01 5 ml
EUR 159.6

2 × Taq Plus Master Mix (Dye Plus)
P212-02 15 ml
EUR 231.6

2 × Taq Plus Master Mix (Dye Plus)
P212-03 50 ml
EUR 466.8