Modulation of Voltage-Gated Sodium Channel (VGSC) Activity in Human Dorsal Root Ganglion (DRG) Neurons by Herpesvirus Quiescent Infection.

The molecular mechanisms of pain associated with alphaherpesvirus latency is unclear. We hypothesize that the voltage-gated sodium channels (VGSC) in dorsal root ganglion (DRG) neurons controlling the electrical impulses may have abnormal activity during infection and reactivation of latent virus. We used HSV-1 infects DRG-derived neural cell line of human HD10.6 to study the formation of viral latency, maintenance and reactivation and changes of VGSC functional expression. differentiated cells exhibited strong tetrodotoxin (TTX) sensitive sodium currents and significantly reduce the acute infection VGSC functional expression within 24 hours, and completely eliminate VGSC activity within 3 days.

A stationary state of infection mimic latency can be achieved in the presence acyclovir (ACV) for 7 days followed by 5 days of washout ACV and then the virus can remain active for three weeks. It was noted that during the formation of HSV-1 latency, loss VGSC activity caused by HSV-1 can not be blocked by the treatment of ACV. However, neurons with ACV treatment continued for 4 days showed a gradual recovery VGSC functional expression.

Furthermore, neurons VGSC latent showed higher activity compared to controls. Overall Regulation VGSC by HSV-1 during a silent infection as evidenced by an increase in the transcription and translation possibilities Nav1.7. Together these observations suggest a very complex pattern of electrophysiological changes during HSV infection DRG neurons, which may have implications for understanding the mechanisms of virus-mediated pain associated with latency and reactivation.Importance: The reactivation of the herpes virus, most often VZV and PRV, can cause nerve disorders cranial and unbearable pain. Clinical studies have also reported that HSV-1 causes occipital neuralgia and chronic postherpetic neuralgia in humans.

Work is currently closely studying the functional VGSC expression profile changes during the process of HSV-1 latency and reactivation formation using human dorsal root ganglion neurons HD 10.6 cells as an in vitro model. Our results show that the activity of VGSC eliminated after infection but continued to recover during the establishment of latency and latency neurons showed VGSC activity is even higher. This finding advances our knowledge of how the ganglion neurons generated electrical impulses as usual for normal VGSC functional expression is affected by latent viruses.

DAPI

BMD0063-10mg 10 mg
EUR 119
Description: DAPI is a popular blue fluorescent DNA dye.

DAPI

B1098-25 each
EUR 496.8

DAPI

B1098-5 each
EUR 170.4

DAPI

abx082063-100l 100 µl
EUR 212.5

DAPI

abx082063-1ml 1 ml Ask for price

DAPI

abx082063-200l 200 µl Ask for price

DAPI

abx283157-100g 100 µg Ask for price

DAPI

abx283157-20g 20 µg
EUR 106.25

DAPI

abx283157-50g 50 µg
EUR 237.5

DAPI

BMD0063-each each Ask for price

DAPI Dilactate

17509 25 mg
EUR 112
Description: DAPI is a fluorescent stain that binds strongly to DNA.

DAPI Dilactate

17509-25mg 25 mg
EUR 109
Description: DAPI is a fluorescent stain that binds strongly to DNA.

DAPI (hydrochloride)

C3362-10 10 mg
EUR 62
Description: A fluorescent probe, commonly used to stain DNA and chromosomes

DAPI (hydrochloride)

C3362-25 25 mg
EUR 130
Description: A fluorescent probe, commonly used to stain DNA and chromosomes

DAPI (hydrochloride)

C3362-5 5 mg
EUR 37
Description: A fluorescent probe, commonly used to stain DNA and chromosomes

DAPI Dihydrochloride

561706 5.0mg
EUR 190

DAPI (dihydrochloride)

HY-D0814 50mg
EUR 439.2

DAPI dihydrochloride

MB097-10MG 1 unit
EUR 41.98
Description: DAPI dihydrochloride

DAPI dihydrochloride

MB097-25MG 1 unit
EUR 83.31
Description: DAPI dihydrochloride

DAPI dihydrochloride

RM5773-25MG 1 unit
EUR 119.85
Description: DAPI dihydrochloride

DAPI Dihydrochloride

T19827-10mg 10mg Ask for price
Description: DAPI Dihydrochloride

DAPI Dihydrochloride

T19827-1g 1g Ask for price
Description: DAPI Dihydrochloride

DAPI Dihydrochloride

T19827-1mg 1mg Ask for price
Description: DAPI Dihydrochloride

DAPI Dihydrochloride

T19827-50mg 50mg Ask for price
Description: DAPI Dihydrochloride

DAPI Dihydrochloride

T19827-5mg 5mg Ask for price
Description: DAPI Dihydrochloride

DAPI Staining Kit

abx097206-1Kit 1 Kit
EUR 276

DAPI Staining Kit

abx097206-100l 100 µl
EUR 162.5

DAPI Staining Kit

abx097206-1ml 1 ml Ask for price

DAPI Staining Kit

abx097206-200l 200 µl Ask for price

FluorLast? with DAPI

1214-20 each
EUR 183.6

DAPI Reference Standard

BLI906A-1 1ml
EUR 265

DAPI Reference Standard

BLI906B-5 5ml
EUR 522

DAPI Reference Standard

BLI906C-14 2x7ml
EUR 1007

DAPI Reagent (25μg/mL)

E-CK-A163-100T 100T
EUR 15

DAPI Reagent (25μg/mL)

E-CK-A163-200T 200T
EUR 25

DAPI Reagent (25μg/mL)

E-CK-A163-500T 500T
EUR 55

DAPI Reagent (25μg/mL)

E-CK-A163-50T 50T
EUR 10

DAPI Solution(1mg/mL)

19178-91 1ML
EUR 38.5

DAPI Reagent (25ug/mL)

E-CK-A163-100Tests 100Tests
EUR 15

DAPI Reagent (25ug/mL)

E-CK-A163-200Tests 200Tests
EUR 25

DAPI Reagent (25ug/mL)

E-CK-A163-500Tests 500Tests
EUR 55

DAPI Reagent (25ug/mL)

E-CK-A163-50Tests 50Tests
EUR 10

DAPI-solution(1mg/ml)

E37F17510DZ 1-mL
EUR 60
Description: Cell-staining.-Nuclear-staining.

Fluoroshield™ with DAPI

AR-6501-01 20 ml
EUR 68.05

Fluoroshield™ with DAPI

AR-6501-02 100 ml
EUR 247.1

Fluoroshield with DAPI & PPD

AR-6513-01 20 ml
EUR 68.05

Fluoroshield with DAPI & PPD

AR-6513-02 100 ml
EUR 231

DAPI (Molecular Biology Grade)

CE117 5 mg
EUR 72

DAPI (Molecular Biology Grade)

CE118 25 mg
EUR 159.6

DAPI (Molecular Biology Grade)

CE119 100 mg
EUR 382.8

Fluoroshield with DAPI & DABCO

AR-6505-01 20 ml
EUR 68.05

Fluoroshield with DAPI & DABCO

AR-6505-02 100 ml
EUR 247.1

DAPI, 10mg/mL in H2O

40043-1 EA
EUR 103

DAPI Reagent (1 ug/mL)

E-IR-R103-10mL 10mL
EUR 35

DAPI Reagent (1 ug/mL)

E-IR-R103-50mL 50mL
EUR 105

DAPI Reagent (1 ug/mL)

E-IR-R103-5mL 5mL
EUR 20

DAPI Reagent (1 ug/mL)

E-IR-R103-each each Ask for price

Fluoroshield with DAPI & Propylgallate

AR-6506-01 20 ml
EUR 68.05

Fluoroshield with DAPI & Propylgallate

AR-6506-02 100 ml
EUR 247.1

FluorLast? with DAPI and DABCO

1218-20 each
EUR 183.6

Fluoromount Mounting Medium with DAPI

42734051-1 20 mL
EUR 76.14

FluoroQuestâ„¢ Mounting Medium with DAPI

20004 50 mL
EUR 112
Description: FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

FluoroQuestâ„¢ Mounting Medium with DAPI

20004-50mL 50 mL
EUR 109
Description: FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

OOSB00007-20ML - Dapi-Fluoromount-G

OOSB00007-20ML 20mL
EUR 79

DAPI staining kit (ready to use) 

EGY0621 10mL
EUR 85

DAPI staining kit (ready to use) 

EGY0622 50mL
EUR 185

Annexin V-PE/DAPI Apoptosis Kit

E-CK-A256-100Assays 100 Assays
EUR 260

Annexin V-PE/DAPI Apoptosis Kit

E-CK-A256-200Assays 200 Assays
EUR 398

Annexin V-PE/DAPI Apoptosis Kit

E-CK-A256-20Assays 20 Assays
EUR 75

Annexin V-PE/DAPI Apoptosis Kit

E-CK-A256-50Assays 50 Assays
EUR 150

Annexin V-APC/DAPI Apoptosis Kit

E-CK-A258-100Assays 100 Assays
EUR 260

Annexin V-APC/DAPI Apoptosis Kit

E-CK-A258-200Assays 200 Assays
EUR 398

Annexin V-APC/DAPI Apoptosis Kit

E-CK-A258-20Assays 20 Assays
EUR 75

Annexin V-APC/DAPI Apoptosis Kit

E-CK-A258-50Assays 50 Assays
EUR 150

Annexin V-FITC/DAPI Apoptosis Kit

E-CK-A252-100Assays 100 Assays
EUR 260

Annexin V-FITC/DAPI Apoptosis Kit

E-CK-A252-200Assays 200 Assays
EUR 398

Annexin V-FITC/DAPI Apoptosis Kit

E-CK-A252-20Assays 20 Assays
EUR 75

Annexin V-FITC/DAPI Apoptosis Kit

E-CK-A252-50Assays 50 Assays
EUR 150

Annexin V-EGFP/DAPI Apoptosis Kit

E-CK-A260-100Assays 100 Assays
EUR 260

Annexin V-EGFP/DAPI Apoptosis Kit

E-CK-A260-200Assays 200 Assays
EUR 398

Annexin V-EGFP/DAPI Apoptosis Kit

E-CK-A260-20Assays 20 Assays
EUR 75

Annexin V-EGFP/DAPI Apoptosis Kit

E-CK-A260-50Assays 50 Assays
EUR 150

Annexin V-AF647/DAPI Apoptosis Kit

E-CK-A254-100Assays 100 Assays
EUR 260

Annexin V-AF647/DAPI Apoptosis Kit

E-CK-A254-200Assays 200 Assays
EUR 398

Annexin V-AF647/DAPI Apoptosis Kit

E-CK-A254-20Assays 20 Assays
EUR 75

Annexin V-AF647/DAPI Apoptosis Kit

E-CK-A254-50Assays 50 Assays
EUR 150

Annexin V-AF488/DAPI Apoptosis Kit

E-CK-A278-100Assays 100 Assays
EUR 260

Annexin V-AF488/DAPI Apoptosis Kit

E-CK-A278-200Assays 200 Assays
EUR 398

Annexin V-AF488/DAPI Apoptosis Kit

E-CK-A278-20Assays 20 Assays
EUR 75

Annexin V-AF488/DAPI Apoptosis Kit

E-CK-A278-50Assays 50 Assays
EUR 150

Annexin V-ER780/DAPI Apoptosis Kit

E-CK-A280-100Assays 100 Assays
EUR 260

Annexin V-ER780/DAPI Apoptosis Kit

E-CK-A280-200Assays 200 Assays
EUR 398

Annexin V-ER780/DAPI Apoptosis Kit

E-CK-A280-20Assays 20 Assays
EUR 75

Annexin V-ER780/DAPI Apoptosis Kit

E-CK-A280-50Assays 50 Assays
EUR 150

Anti-Fade Mounting Medium (With DAPI)

abx090690-10ml 10 ml
EUR 243.6

Anti-Fade Mounting Medium (With DAPI)

abx090690-100l 100 µl
EUR 137.5

Anti-Fade Mounting Medium (With DAPI)

abx090690-1ml 1 ml Ask for price

Anti-Fade Mounting Medium (With DAPI)

abx090690-200l 200 µl Ask for price

DAPI in Fluorguard™ (0.3 µg/ml)

F201 1 ml Ask for price

DAPI in Fluorguard™ (0.5 µg/ml)

F203 1 ml Ask for price

DAPI in Fluorguard™ (1 µg/ml)

F204 1 ml Ask for price

EverBrite™ Mounting Medium with DAPI

23002-1 EA
EUR 172

Annexin V-Cyanine5/DAPI Apoptosis Kit

E-CK-A262-100Assays 100 Assays
EUR 260

Annexin V-Cyanine5/DAPI Apoptosis Kit

E-CK-A262-200Assays 200 Assays
EUR 398

Annexin V-Cyanine5/DAPI Apoptosis Kit

E-CK-A262-20Assays 20 Assays
EUR 75

Annexin V-Cyanine5/DAPI Apoptosis Kit

E-CK-A262-50Assays 50 Assays
EUR 150

Annexin V-PE/TR/DAPI Apoptosis Kit

E-CK-A272-100Assays 100 Assays
EUR 260

Annexin V-PE/TR/DAPI Apoptosis Kit

E-CK-A272-200Assays 200 Assays
EUR 398

Annexin V-PE/TR/DAPI Apoptosis Kit

E-CK-A272-20Assays 20 Assays
EUR 75

Annexin V-PE/TR/DAPI Apoptosis Kit

E-CK-A272-50Assays 50 Assays
EUR 150

Immuno Fluoro Mount with DABCO and DAPI

AR-6519-01 20 ml
EUR 68.05

Immuno Fluoro Mount with DABCO and DAPI

AR-6519-02 100 ml
EUR 207.2

Annexin V-PE/Cyanine5/DAPI Apoptosis Kit

E-CK-A264-100Assays 100 Assays
EUR 260

Annexin V-PE/Cyanine5/DAPI Apoptosis Kit

E-CK-A264-200Assays 200 Assays
EUR 398

Annexin V-PE/Cyanine5/DAPI Apoptosis Kit

E-CK-A264-20Assays 20 Assays
EUR 75

Annexin V-PE/Cyanine5/DAPI Apoptosis Kit

E-CK-A264-50Assays 50 Assays
EUR 150

Annexin V-PE/Cyanine5.5/DAPI Apoptosis Kit

E-CK-A266-100Assays 100 Assays
EUR 260

Annexin V-PE/Cyanine5.5/DAPI Apoptosis Kit

E-CK-A266-200Assays 200 Assays
EUR 398

Annexin V-PE/Cyanine5.5/DAPI Apoptosis Kit

E-CK-A266-20Assays 20 Assays
EUR 75

Annexin V-PE/Cyanine5.5/DAPI Apoptosis Kit

E-CK-A266-50Assays 50 Assays
EUR 150

Annexin V-PE/Cyanine7/DAPI Apoptosis Kit

E-CK-A268-100Assays 100 Assays
EUR 260

Annexin V-PE/Cyanine7/DAPI Apoptosis Kit

E-CK-A268-200Assays 200 Assays
EUR 398

Annexin V-PE/Cyanine7/DAPI Apoptosis Kit

E-CK-A268-20Assays 20 Assays
EUR 75

Annexin V-PE/Cyanine7/DAPI Apoptosis Kit

E-CK-A268-50Assays 50 Assays
EUR 150

Annexin V-APC/Cyanine7/DAPI Apoptosis Kit

E-CK-A270-100Assays 100 Assays
EUR 260

Annexin V-APC/Cyanine7/DAPI Apoptosis Kit

E-CK-A270-200Assays 200 Assays
EUR 398

Annexin V-APC/Cyanine7/DAPI Apoptosis Kit

E-CK-A270-20Assays 20 Assays
EUR 75

Annexin V-APC/Cyanine7/DAPI Apoptosis Kit

E-CK-A270-50Assays 50 Assays
EUR 150

Fluorescent Mounting Medium with DAPI, 18 ml

E19-18 -
EUR 125

Glycerol mounting medium with DAPI and DABCO

AR-6510-01 20 ml
EUR 68.05

Glycerol mounting medium with DAPI and DABCO

AR-6510-02 100 ml
EUR 207.2

DAPI (4',6-Diamidino-2-phenylindole dihy

PCT1539-25MG 1 unit
EUR 77.94
Description: DAPI (4',6-Diamidino-2-phenylindole dihy

DAPI (4',6-Diamidino-2-phenylindole dihy

PCT1539-5MG 1 unit
EUR 18.69
Description: DAPI (4',6-Diamidino-2-phenylindole dihy

EverBrite™ Hardset Mounting Medium with DAPI

23004-1 EA
EUR 194

FluoroQuestâ„¢ Anti-fading Mounting Medium with DAPI

20005 20 mL
EUR 216
Description: FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

FluoroQuestâ„¢ Anti-fading Mounting Medium with DAPI

20005-20mL 20 mL
EUR 211
Description: FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Anti-fluorescence quenching sealing Kit (including DAPI)

EGY073 100T
EUR 60

DAPI-[4',6-Diamidino-2-phenylindole,-dihydrochloride]

E37F17510 10-mg
EUR 260
Description: Cell-staining.-Nuclear-staining.

DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)

B551 0.2 ml
EUR 85

Dye 937

HY-15422 5mg
EUR 514.8

Dye 993

HY-15423 5mg
EUR 514.8

Dye 937

T18985-10mg 10mg Ask for price
Description: Dye 937

Dye 937

T18985-1g 1g Ask for price
Description: Dye 937

Dye 937

T18985-1mg 1mg Ask for price
Description: Dye 937

Dye 937

T18985-50mg 50mg Ask for price
Description: Dye 937

Dye 937

T18985-5mg 5mg Ask for price
Description: Dye 937

Dye 993

T18986-10mg 10mg Ask for price
Description: Dye 993

Dye 993

T18986-1g 1g Ask for price
Description: Dye 993

Dye 993

T18986-1mg 1mg Ask for price
Description: Dye 993

Dye 993

T18986-50mg 50mg Ask for price
Description: Dye 993