The molecular mechanisms of pain associated with alphaherpesvirus latency is unclear. We hypothesize that the voltage-gated sodium channels (VGSC) in dorsal root ganglion (DRG) neurons controlling the electrical impulses may have abnormal activity during infection and reactivation of latent virus. We used HSV-1 infects DRG-derived neural cell line of human HD10.6 to study the formation of viral latency, maintenance and reactivation and changes of VGSC functional expression. differentiated cells exhibited strong tetrodotoxin (TTX) sensitive sodium currents and significantly reduce the acute infection VGSC functional expression within 24 hours, and completely eliminate VGSC activity within 3 days.
A stationary state of infection mimic latency can be achieved in the presence acyclovir (ACV) for 7 days followed by 5 days of washout ACV and then the virus can remain active for three weeks. It was noted that during the formation of HSV-1 latency, loss VGSC activity caused by HSV-1 can not be blocked by the treatment of ACV. However, neurons with ACV treatment continued for 4 days showed a gradual recovery VGSC functional expression.
Furthermore, neurons VGSC latent showed higher activity compared to controls. Overall Regulation VGSC by HSV-1 during a silent infection as evidenced by an increase in the transcription and translation possibilities Nav1.7. Together these observations suggest a very complex pattern of electrophysiological changes during HSV infection DRG neurons, which may have implications for understanding the mechanisms of virus-mediated pain associated with latency and reactivation.Importance: The reactivation of the herpes virus, most often VZV and PRV, can cause nerve disorders cranial and unbearable pain. Clinical studies have also reported that HSV-1 causes occipital neuralgia and chronic postherpetic neuralgia in humans.
Work is currently closely studying the functional VGSC expression profile changes during the process of HSV-1 latency and reactivation formation using human dorsal root ganglion neurons HD 10.6 cells as an in vitro model. Our results show that the activity of VGSC eliminated after infection but continued to recover during the establishment of latency and latency neurons showed VGSC activity is even higher. This finding advances our knowledge of how the ganglion neurons generated electrical impulses as usual for normal VGSC functional expression is affected by latent viruses.
 2X PCR HotStart MasterMix with dye |
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G906-dye |
ABM |
5.0 ml (200 Rxns) |
EUR 158 |
 2X PCR Taq Plus MasterMix with dye |
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G014-dye |
ABM |
5.0 ml (200 Rxns) |
EUR 106 |
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G124-dye |
ABM |
5.0 ml (200 Rxns) |
EUR 163 |
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G174-dye |
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100 x 50 ul reactions |
EUR 233 |
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G464-dye |
ABM |
5.0 ml (200 Rxns) |
EUR 158 |
2X PCR Taq Plus MasterMix with dye |
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G901-dye |
ABM |
25.0 ml (1000 Rxns) |
EUR 299 |
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G939-Dye |
ABM |
5.0 ml (200 Rxns) |
EUR 158 |
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abx082063-10mg |
Abbexa |
10 mg |
EUR 230 |
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DAPI |
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40011 |
Biotium |
10MG |
EUR 140 |
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Description: Minimum order quantity: 1 unit of 10MG |
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C3362-10 |
ApexBio |
10 mg |
EUR 122 |
DAPI (hydrochloride) |
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C3362-25 |
ApexBio |
25 mg |
EUR 200 |
DAPI (hydrochloride) |
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C3362-5 |
ApexBio |
5 mg |
EUR 93 |
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abx097206-1Kit |
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1 Kit |
EUR 230 |
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1214-20 |
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EUR 153 |
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CE117 |
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25 mg |
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CE119 |
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EUR 319 |
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1-CSB-PA772049LA01DIL |
Cusabio |
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Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:5000 |
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85R-1016 |
Fitzgerald |
1 mg |
EUR 133 |
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Description: Phycocyanin dye for use in conjugation to biomolecules and antibodies |
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85R-1018 |
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1 mg |
EUR 133 |
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Description: Phycocyanin dye for use in conjugation to biomolecules and antibodies |
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23002 |
Biotium |
10mL |
EUR 195 |
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Description: Minimum order quantity: 1 unit of 10mL |
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40043 |
Biotium |
1ML |
EUR 134 |
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1218-20 |
Biovision |
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EUR 153 |
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20-abx098002 |
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-
EUR 411.00
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EUR 189.00
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EUR 258.00
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) PCR SuperMix (+dye) |
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20-abx098003 |
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EUR 411.00
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EUR 189.00
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EUR 6174.00
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EUR 258.00
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SB2040 |
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100UL, 100UL |
EUR 67.4 |
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G030 |
ABM |
6X; 3 x 1.0 ml |
EUR 79 |
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0209R |
AthenaES |
100 ml |
EUR 131 |
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abx090690-10ml |
Abbexa |
10 ml |
EUR 203 |
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23004 |
Biotium |
10mL |
EUR 213 |
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Description: Minimum order quantity: 1 unit of 10mL |
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20004 |
AAT Bioquest |
50 mL |
EUR 132 |
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IB0280 |
Bio Basic |
250mg |
EUR 63.05 |
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F553 |
GeneOn |
1 ml (10µM) |
EUR 61 |
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EUR 172 |
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20-abx098005 |
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20-abx098006 |
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20-abx098009 |
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20-abx098010 |
Abbexa |
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29052 |
Biotium |
5mL |
EUR 195 |
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Description: Minimum order quantity: 1 unit of 5mL |
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R1000-500 |
GenDepot |
500ul |
EUR 115 |
 dye Antibody, HRP conjugated |
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1-CSB-PA772049LB01DIL |
Cusabio |
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Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is HRP conjugated. Tested in the following application: ELISA |
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1-CSB-PA772049LC01DIL |
Cusabio |
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Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is FITC conjugated. Tested in the following application: ELISA |
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1-CSB-PA772049LD01DIL |
Cusabio |
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Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is Biotin conjugated. Tested in the following application: ELISA |
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G924 |
ABM |
6X; 3 x 1.0 ml |
EUR 79 |
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M1128-1000 |
Biovision |
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EUR 512 |
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EUR 191 |
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M1133-200 |
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EUR 321 |
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EUR 267 |
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EUR 294 |
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EUR 376 |
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85R-1017 |
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EUR 122 |
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Description: Cross-linked phycocyanin dye for use in conjugation to biomolecules and antibodies |
) DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) |
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B551 |
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0.2 ml |
EUR 108 |
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20-abx098004 |
Abbexa |
-
EUR 411.00
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EUR 189.00
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EUR 258.00
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FYG603-50P |
Yeastern Biotech |
50 Preps |
Ask for price |
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V1004-001 |
GenDepot |
1ml |
EUR 73 |
iVDye 6X Gel Loading Dye |
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V1004-010 |
GenDepot |
10x1ml |
EUR 178 |
iVDye 6X Gel Loading Dye |
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V1004-020 |
GenDepot |
20x1ml |
EUR 215 |
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30021 |
Biotium |
1ML |
EUR 191 |
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Description: Minimum order quantity: 1 unit of 1ML |
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30022 |
Biotium |
1ML |
EUR 191 |
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Description: Minimum order quantity: 1 unit of 1ML |
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30023 |
Biotium |
1ML |
EUR 191 |
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Description: Minimum order quantity: 1 unit of 1ML |
 Powerpol 2xPCR mix with dye |
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RK20719 |
Abclonal |
1mL |
Ask for price |
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G447 |
ABM |
250 ml |
EUR 254 |
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M1131-1000 |
Biovision |
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EUR 615 |
Robust Ready? PCR Mix-Dye |
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M1131-200 |
Biovision |
|
EUR 213 |
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M1142-200 |
Biovision |
|
EUR 376 |
) DAPI, dilactate (4’, 6-diamidino-2-phenylindole, dilactate) |
|
40009 |
Biotium |
10MG |
EUR 146 |
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Description: Minimum order quantity: 1 unit of 10MG |
) ROX Reference Dye (25 µM; 50x) |
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F153 |
GeneOn |
1 ml |
EUR 61 |
ROX Reference Dye (25 µM; 50x) |
|
F153L |
GeneOn |
5x1 ml |
EUR 172 |
 Green-DNA Dye, ready to use |
|
DT81414 |
Bio Basic |
1.5ml, 1.5ml |
EUR 128.3 |
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) Top Green qPCR SuperMix (+Dye II) |
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20-abx098890 |
Abbexa |
-
EUR 829.00
-
EUR 217.00
-
EUR 1135.00
-
EUR 411.00
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) Tip Green qPCR SuperMix (+Dye II) |
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20-abx098891 |
Abbexa |
-
EUR 968.00
-
EUR 230.00
-
EUR 1330.00
-
EUR 467.00
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 Load 6x Loading Dye Bromophenol Blue |
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BA01401 |
Bioatlas |
6x1ml |
EUR 80 |
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Description: High purity buffer for various PCR applications. |
 Load 6x Loading Dye Orange G |
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BA01402 |
Bioatlas |
6x1ml |
EUR 80 |
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Description: High purity buffer for various PCR applications. |
 Fast Red Violet Dye for IHC |
|
G446 |
ABM |
250 ml |
EUR 342 |
 Easy? One Step RT Kit-Dye |
|
M1163-100 |
Biovision |
|
EUR 468 |
) 2 × Taq Master Mix (Dye Plus) |
|
P112-01 |
Vazyme |
5 ml |
EUR 117 |
2 × Taq Master Mix (Dye Plus) |
|
P112-02 |
Vazyme |
15 ml |
EUR 146 |
2 × Taq Master Mix (Dye Plus) |
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P112-03 |
Vazyme |
50 ml |
EUR 236 |
) 2 × AceTaq Master Mix (Dye Plus) |
|
P412-01 |
Vazyme |
1 ml |
EUR 119 |
2 × AceTaq Master Mix (Dye Plus) |
|
P412-02 |
Vazyme |
5 ml |
EUR 158 |
2 × AceTaq Master Mix (Dye Plus) |
|
P412-03 |
Vazyme |
15 ml |
EUR 262 |
 TaqProbe 5X qPCR MasterMix-No Dye |
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MasterMix-5PS |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
 BrightGreen 5X qPCR MasterMix-No Dye |
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MasterMix-5S |
ABM |
4 x 1.0 ml for 1000 reactions (20 ul) |
EUR 213 |
 KiloGreen 2X qPCR MasterMix-No Dye |
|
MasterMix-KS |
ABM |
4 x 1.25 ml - 500 reactions (20 ul) |
EUR 140 |
 BrightGreen miRNA qPCR MasterMix-No Dye |
|
MasterMix-mS |
ABM |
4 x 1.25 ml for 500 reactions |
EUR 151 |
 TaqProbe 2X qPCR MasterMix-No Dye |
|
MasterMix-PS |
ABM |
4 x 1.25 ml for 500 reactions (20 ul) |
EUR 140 |
