Modulation of Voltage-Gated Sodium Channel (VGSC) Activity in Human Dorsal Root Ganglion (DRG) Neurons by Herpesvirus Quiescent Infection.

The molecular mechanisms of pain associated with alphaherpesvirus latency is unclear. We hypothesize that the voltage-gated sodium channels (VGSC) in dorsal root ganglion (DRG) neurons controlling the electrical impulses may have abnormal activity during infection and reactivation of latent virus. We used HSV-1 infects DRG-derived neural cell line of human HD10.6 to study the formation of viral latency, maintenance and reactivation and changes of VGSC functional expression. differentiated cells exhibited strong tetrodotoxin (TTX) sensitive sodium currents and significantly reduce the acute infection VGSC functional expression within 24 hours, and completely eliminate VGSC activity within 3 days.

A stationary state of infection mimic latency can be achieved in the presence acyclovir (ACV) for 7 days followed by 5 days of washout ACV and then the virus can remain active for three weeks. It was noted that during the formation of HSV-1 latency, loss VGSC activity caused by HSV-1 can not be blocked by the treatment of ACV. However, neurons with ACV treatment continued for 4 days showed a gradual recovery VGSC functional expression.

Furthermore, neurons VGSC latent showed higher activity compared to controls. Overall Regulation VGSC by HSV-1 during a silent infection as evidenced by an increase in the transcription and translation possibilities Nav1.7. Together these observations suggest a very complex pattern of electrophysiological changes during HSV infection DRG neurons, which may have implications for understanding the mechanisms of virus-mediated pain associated with latency and reactivation.Importance: The reactivation of the herpes virus, most often VZV and PRV, can cause nerve disorders cranial and unbearable pain. Clinical studies have also reported that HSV-1 causes occipital neuralgia and chronic postherpetic neuralgia in humans.

Work is currently closely studying the functional VGSC expression profile changes during the process of HSV-1 latency and reactivation formation using human dorsal root ganglion neurons HD 10.6 cells as an in vitro model. Our results show that the activity of VGSC eliminated after infection but continued to recover during the establishment of latency and latency neurons showed VGSC activity is even higher. This finding advances our knowledge of how the ganglion neurons generated electrical impulses as usual for normal VGSC functional expression is affected by latent viruses.

2X PCR HotStart MasterMix with dye

G906-dye 5.0 ml (200 Rxns)
EUR 158

2X PCR Taq Plus MasterMix with dye

G014-dye 5.0 ml (200 Rxns)
EUR 106

2X PCR Precision™ MasterMix with dye

G124-dye 5.0 ml (200 Rxns)
EUR 163

One-Step RT-PCR Kit with dye

G174-dye 100 x 50 ul reactions
EUR 233

2X PCR Bestaq™ MasterMix with dye

G464-dye 5.0 ml (200 Rxns)
EUR 158

2X PCR Taq Plus MasterMix with dye

G901-dye 25.0 ml (1000 Rxns)
EUR 299

2X PCR SensTaq HotStart MasterMix with Dye

G939-Dye 5.0 ml (200 Rxns)
EUR 158

DAPI

abx082063-10mg 10 mg
EUR 230

DAPI

40011 10MG
EUR 140
Description: Minimum order quantity: 1 unit of 10MG

DAPI

B1098-25
EUR 414

DAPI

B1098-5
EUR 142

DAPI (dihydrochloride)

HY-D0814 50mg
EUR 366

DAPI Dilactate

17509 25 mg
EUR 132

DAPI (hydrochloride)

C3362-10 10 mg
EUR 122

DAPI (hydrochloride)

C3362-25 25 mg
EUR 200

DAPI (hydrochloride)

C3362-5 5 mg
EUR 93

DAPI Staining Kit

abx097206-1Kit 1 Kit
EUR 230

FluorLast? with DAPI

1214-20
EUR 153

DAPI (Molecular Biology Grade)

CE117 5 mg
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DAPI (Molecular Biology Grade)

CE118 25 mg
EUR 133

DAPI (Molecular Biology Grade)

CE119 100 mg
EUR 319

Dye 937

HY-15422 5mg
EUR 429

Dye 993

HY-15423 5mg
EUR 429

D149 Dye

HY-50938 50mg
EUR 271

dye Antibody

1-CSB-PA772049LA01DIL
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  • EUR 335.00
  • 100ug
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Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:5000

Phycocyanin dye

85R-1016 1 mg
EUR 133
Description: Phycocyanin dye for use in conjugation to biomolecules and antibodies

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85R-1018 1 mg
EUR 133
Description: Phycocyanin dye for use in conjugation to biomolecules and antibodies

EverBrite Mounting Medium with DAPI

23002 10mL
EUR 195
Description: Minimum order quantity: 1 unit of 10mL

DAPI, 10mg/mL in H2O

40043 1ML
EUR 134
Description: Minimum order quantity: 1 unit of 1ML

FluorLast? with DAPI and DABCO

1218-20
EUR 153

PCR SuperMix (-dye)

20-abx098002
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  • EUR 258.00
  • 15 ml
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PCR SuperMix (+dye)

20-abx098003
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  • EUR 189.00
  • EUR 6174.00
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  • 15 ml
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  • 480 ml
  • 5 ml

Green-DNA Dye

SB2040 100UL, 100UL
EUR 67.4

DNA Loading Dye

G030 6X; 3 x 1.0 ml
EUR 79

Bradford Dye Reagent

0209R 100 ml
EUR 131

Anti-Fade Mounting Medium (With DAPI)

abx090690-10ml 10 ml
EUR 203

EverBrite Hardset Mounting Medium with DAPI

23004 10mL
EUR 213
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FluoroQuest™ Mounting Medium with DAPI

20004 50 mL
EUR 132

INT dye (Iodonitrotetrazolium chloride)

IB0280 250mg
EUR 63.05

Fluorescein Reference Dye (100X)

F553 1 ml (10µM)
EUR 61

Fluorescein Reference Dye (100X)

F553L 5x1 ml (50µM)
EUR 172

Heptamethine cyanine dye-1

HY-D0921 50mg
EUR 2116

T PCR SuperMix (-dye)

20-abx098005
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  • EUR 342.00
  • 1 ml
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T PCR SuperMix (+dye)

20-abx098006
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  • EUR 342.00
  • 1 ml
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EasyPfu PCR SuperMix (-dye)

20-abx098009
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  • EUR 342.00
  • 1 ml
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FastPfu PCR SuperMix (-dye)

20-abx098010
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  • EUR 495.00
  • 1 ml
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Gel Loading Dye Solution

FYD003-3ML 3 ml Ask for price

ROX passive reference dye

29052 5mL
EUR 195
Description: Minimum order quantity: 1 unit of 5mL

Rox Passive Reference Dye

R1000-500 500ul
EUR 115

dye Antibody, HRP conjugated

1-CSB-PA772049LB01DIL
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is HRP conjugated. Tested in the following application: ELISA

dye Antibody, FITC conjugated

1-CSB-PA772049LC01DIL
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is FITC conjugated. Tested in the following application: ELISA

dye Antibody, Biotin conjugated

1-CSB-PA772049LD01DIL
  • EUR 317.00
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  • 100ug
  • 50ug
Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is Biotin conjugated. Tested in the following application: ELISA

DNA Loading Green Dye

G924 6X; 3 x 1.0 ml
EUR 79

Ready? PCR Mix-Dye

M1128-1000
EUR 512

Ready? PCR Mix-Dye

M1128-200
EUR 191

Rigor? PCR Mix-Dye

M1133-200
EUR 321

Breeze? PCR Mix-Dye

M1135-200
EUR 267

Distant? PCR Mix-Dye

M1137-200
EUR 294

Advance? PCR Mix-Dye

M1140-200
EUR 376

Phycocyanin dye (cross-linked)

85R-1017 1 mg
EUR 122
Description: Cross-linked phycocyanin dye for use in conjugation to biomolecules and antibodies

DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)

B551 0.2 ml
EUR 108

PCR SuperMix for PAGE (+dye)

20-abx098004
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  • 15 ml
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G50 Dye Terminator Removal Kit

FYG603-50P 50 Preps Ask for price

iVDye 6X Gel Loading Dye

V1004-001 1ml
EUR 73

iVDye 6X Gel Loading Dye

V1004-010 10x1ml
EUR 178

iVDye 6X Gel Loading Dye

V1004-020 20x1ml
EUR 215

CELLBRITE GREEN CYTOPLASMIC MEMBRANE DYE

30021 1ML
EUR 191
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CELLBRITE ORANGE CYTOPLASMIC MEMBRANE DYE

30022 1ML
EUR 191
Description: Minimum order quantity: 1 unit of 1ML

CellBriteTM Red Cytoplasmic Membrane Dye

30023 1ML
EUR 191
Description: Minimum order quantity: 1 unit of 1ML

Powerpol 2xPCR mix with dye

RK20719 1mL Ask for price

BCIP-NBT Dye for IHC

G447 250 ml
EUR 254

Robust Ready? PCR Mix-Dye

M1131-1000
EUR 615

Robust Ready? PCR Mix-Dye

M1131-200
EUR 213

Fire Start? PCR Mix-Dye

M1142-200
EUR 376

DAPI, dilactate (4’, 6-diamidino-2-phenylindole, dilactate)

40009 10MG
EUR 146
Description: Minimum order quantity: 1 unit of 10MG

ROX Reference Dye (25 µM; 50x)

F153 1 ml
EUR 61

ROX Reference Dye (25 µM; 50x)

F153L 5x1 ml
EUR 172

Green-DNA Dye, ready to use

DT81414 1.5ml, 1.5ml
EUR 128.3

Top Green qPCR SuperMix (+Dye II)

20-abx098890
  • EUR 829.00
  • EUR 217.00
  • EUR 1135.00
  • EUR 411.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Tip Green qPCR SuperMix (+Dye II)

20-abx098891
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  • EUR 230.00
  • EUR 1330.00
  • EUR 467.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Load 6x Loading Dye Bromophenol Blue

BA01401 6x1ml
EUR 80
Description: High purity buffer for various PCR applications.

Load 6x Loading Dye Orange G

BA01402 6x1ml
EUR 80
Description: High purity buffer for various PCR applications.

1.2ML Triple Dye Loading Buffer (6X)

NAT1192 1.2ML
EUR 71

Fast Red Violet Dye for IHC

G446 250 ml
EUR 342

Easy? One Step RT Kit-Dye

M1163-100
EUR 468

2 × Taq Master Mix (Dye Plus)

P112-01 5 ml
EUR 117

2 × Taq Master Mix (Dye Plus)

P112-02 15 ml
EUR 146

2 × Taq Master Mix (Dye Plus)

P112-03 50 ml
EUR 236

2 × AceTaq Master Mix (Dye Plus)

P412-01 1 ml
EUR 119

2 × AceTaq Master Mix (Dye Plus)

P412-02 5 ml
EUR 158

2 × AceTaq Master Mix (Dye Plus)

P412-03 15 ml
EUR 262

TaqProbe 5X qPCR MasterMix-No Dye

MasterMix-5PS 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen 5X qPCR MasterMix-No Dye

MasterMix-5S 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

KiloGreen 2X qPCR MasterMix-No Dye

MasterMix-KS 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

BrightGreen miRNA qPCR MasterMix-No Dye

MasterMix-mS 4 x 1.25 ml for 500 reactions
EUR 151

TaqProbe 2X qPCR MasterMix-No Dye

MasterMix-PS 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140