Modulation of Voltage-Gated Sodium Channel (VGSC) Activity in Human Dorsal Root Ganglion (DRG) Neurons by Herpesvirus Quiescent Infection.

The molecular mechanisms of pain associated with alphaherpesvirus latency is unclear. We hypothesize that the voltage-gated sodium channels (VGSC) in dorsal root ganglion (DRG) neurons controlling the electrical impulses may have abnormal activity during infection and reactivation of latent virus. We used HSV-1 infects DRG-derived neural cell line of human HD10.6 to study the formation of viral latency, maintenance and reactivation and changes of VGSC functional expression. differentiated cells exhibited strong tetrodotoxin (TTX) sensitive sodium currents and significantly reduce the acute infection VGSC functional expression within 24 hours, and completely eliminate VGSC activity within 3 days.

A stationary state of infection mimic latency can be achieved in the presence acyclovir (ACV) for 7 days followed by 5 days of washout ACV and then the virus can remain active for three weeks. It was noted that during the formation of HSV-1 latency, loss VGSC activity caused by HSV-1 can not be blocked by the treatment of ACV. However, neurons with ACV treatment continued for 4 days showed a gradual recovery VGSC functional expression.

Furthermore, neurons VGSC latent showed higher activity compared to controls. Overall Regulation VGSC by HSV-1 during a silent infection as evidenced by an increase in the transcription and translation possibilities Nav1.7. Together these observations suggest a very complex pattern of electrophysiological changes during HSV infection DRG neurons, which may have implications for understanding the mechanisms of virus-mediated pain associated with latency and reactivation.Importance: The reactivation of the herpes virus, most often VZV and PRV, can cause nerve disorders cranial and unbearable pain. Clinical studies have also reported that HSV-1 causes occipital neuralgia and chronic postherpetic neuralgia in humans.

Work is currently closely studying the functional VGSC expression profile changes during the process of HSV-1 latency and reactivation formation using human dorsal root ganglion neurons HD 10.6 cells as an in vitro model. Our results show that the activity of VGSC eliminated after infection but continued to recover during the establishment of latency and latency neurons showed VGSC activity is even higher. This finding advances our knowledge of how the ganglion neurons generated electrical impulses as usual for normal VGSC functional expression is affected by latent viruses.

DAPI Dilactate
17509-25mg	AAT Bioquest	25 mg	EUR 109
Description: DAPI is a fluorescent stain that binds strongly to DNA.

DAPI (hydrochloride)
C3362-10	ApexBio	10 mg	EUR 146.4

DAPI (hydrochloride)
C3362-25	ApexBio	25 mg	EUR 240

DAPI (hydrochloride)
C3362-5	ApexBio	5 mg	EUR 111.6

DAPI (dihydrochloride)
HY-D0814	MedChemExpress	50mg	EUR 439.2

DAPI Staining Kit
abx097206-1Kit	Abbexa	1 Kit	EUR 276

FluorLast? with DAPI
1214-20	Biovision	each	EUR 183.6

DAPI (Molecular Biology Grade)
CE117	GeneOn	5 mg	EUR 72

DAPI (Molecular Biology Grade)
CE118	GeneOn	25 mg	EUR 159.6

DAPI (Molecular Biology Grade)
CE119	GeneOn	100 mg	EUR 382.8

FluorLast? with DAPI and DABCO
1218-20	Biovision	each	EUR 183.6

FluoroQuestâ„¢ Mounting Medium with DAPI
20004-50mL	AAT Bioquest	50 mL	EUR 109
Description: FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Anti-Fade Mounting Medium (With DAPI)
abx090690-10ml	Abbexa	10 ml	EUR 243.6

FluoroQuest™ Mounting Medium with DAPI
20004	AAT Bioquest	50 mL	EUR 109

EverBrite Hardset Mounting Medium with DAPI
23004	Biotium	10mL	EUR 255.6
Description: Minimum order quantity: 1 unit of 10mL

FluoroQuestâ„¢ Anti-fading Mounting Medium with DAPI
20005-20mL	AAT Bioquest	20 mL	EUR 211
Description: FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)
B551	ABM	0.2 ml	EUR 129.6

FluoroQuest™ Anti-fading Mounting Medium with DAPI
20005	AAT Bioquest	20 mL	EUR 211

Dye 937
HY-15422	MedChemExpress	5mg	EUR 514.8

Dye 993
HY-15423	MedChemExpress	5mg	EUR 514.8

D149 Dye
HY-50938	MedChemExpress	50mg	EUR 325.2

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*
17510	AAT Bioquest	10 mg	EUR 86

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*
17511	AAT Bioquest	100 mg	EUR 222

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*
17513	AAT Bioquest	25 mg	EUR 109

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*
17510-10mg	AAT Bioquest	10 mg	EUR 86
Description: DAPI is a fluorescent stain that binds strongly to DNA.

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*
17511-100mg	AAT Bioquest	100 mg	EUR 222
Description: DAPI is a fluorescent stain that binds strongly to DNA.

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*
17513-25mg	AAT Bioquest	25 mg	EUR 109
Description: DAPI is a fluorescent stain that binds strongly to DNA.

dye Antibody
1-CSB-PA772049LA01DIL	Cusabio	EUR 380.40 EUR 402.00	100ug 50ug
Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:5000

Human 4’,6-Diamidino-2-phenylindole dihydrochloride（DAPI)ELISA kit
QY-E05538	Qayee Biotechnology	96T	EUR 433.2

Phycocyanin dye
85R-1016	Fitzgerald	1 mg	EUR 159.6
Description: Phycocyanin dye for use in conjugation to biomolecules and antibodies

Phycoerythrin dye
85R-1018	Fitzgerald	1 mg	EUR 159.6
Description: Phycocyanin dye for use in conjugation to biomolecules and antibodies

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *10 mM solution in water*
17507	AAT Bioquest	2 mL	EUR 86

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *10 mM solution in water*
17507-2mL	AAT Bioquest	2 mL	EUR 86
Description: DAPI is a fluorescent stain that binds strongly to DNA.

DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining
AR1176	BosterBio	10mL(100 assys)	EUR 78

DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining
AR1177	BosterBio	50mL(500 assys)	EUR 121.2

Dye removal bag
DD4905007	Scientific Laboratory Supplies	EACH	EUR 236.4

DNA Loading Dye
G030	ABM	6X; 3 x 1.0 ml	EUR 94.8

Dye Solution A
IRDSA01	Scientific Laboratory Supplies	100ML	EUR 231.6

Green-DNA Dye
SB2040	Bio Basic	100UL, 100UL	EUR 80.88

PCR SuperMix (+dye)
20-abx098003	Abbexa	EUR 493.20 EUR 226.80 EUR 7408.80 EUR 309.60	15 ml 1 ml 480 ml 5 ml

PCR SuperMix (-dye)
20-abx098002	Abbexa	EUR 493.20 EUR 226.80 EUR 309.60	15 ml 1 ml 5 ml

Bradford Dye Reagent
0209R	AthenaES	100 ml	EUR 157.2

LAMP Fluorescent Dye
RP001-01	Vazyme	100 μl	EUR 12.4

Heptamethine cyanine dye-1
HY-D0921	MedChemExpress	50mg	EUR 2539.2

Fluorescein Reference Dye (100X)
F553	GeneOn	1 ml (10µM)	EUR 73.2

Fluorescein Reference Dye (100X)
F553L	GeneOn	5x1 ml (50µM)	EUR 206.4

Liposomal Fluorescein Dye
DPC001FL	ProFoldin	1 ml	EUR 140.62
Description: This product includes 1 ml of 0.1 mM Fluorescein encapsulated in liposome composed of DPPC:Cholesterol (w/w 1:1).

Cye Dye Bulk Pack
PA55322	Scientific Laboratory Supplies	EACH	EUR 3968.4

T PCR SuperMix (-dye)
20-abx098005	Abbexa	EUR 243.60 EUR 410.40	1 ml 5 ml

T PCR SuperMix (+dye)
20-abx098006	Abbexa	EUR 243.60 EUR 410.40	1 ml 5 ml

DNA Loading Green Dye
G924	ABM	6X; 3 x 1.0 ml	EUR 94.8

EasyPfu PCR SuperMix (-dye)
20-abx098009	Abbexa	EUR 243.60 EUR 410.40	1 ml 5 ml

FastPfu PCR SuperMix (-dye)
20-abx098010	Abbexa	EUR 292.80 EUR 594.00	1 ml 5 ml

Gel Loading Dye Solution
FYD003-3ML	Yeastern Biotech	3 ml	Ask for price

ROX passive reference dye
29052	Biotium	5mL	EUR 234
Description: Minimum order quantity: 1 unit of 5mL

Rox Passive Reference Dye
R1000-500	GenDepot	500ul	EUR 138

Liposomal Rhodamine G Dye
DPC001RG	ProFoldin	1 ml	EUR 140.62
Description: This product includes 1 ml of 0.1 mM Rhodamine G encapsulated in liposome composed of DPPC:Cholesterol (w/w 1:1).

dye Antibody, HRP conjugated
1-CSB-PA772049LB01DIL	Cusabio	EUR 380.40 EUR 402.00	100ug 50ug
Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is HRP conjugated. Tested in the following application: ELISA

dye Antibody, FITC conjugated
1-CSB-PA772049LC01DIL	Cusabio	EUR 380.40 EUR 402.00	100ug 50ug
Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is FITC conjugated. Tested in the following application: ELISA

Liposomal Acridine Orange Dye
DPC001AO	ProFoldin	1 ml	EUR 140.62
Description: This product includes 1 ml of 0.1 mM acridine orange encapsulated in liposome composed of DPPC:Cholesterol (w/w 1:1).

Phycocyanin dye (cross-linked)
85R-1017	Fitzgerald	1 mg	EUR 146.4
Description: Cross-linked phycocyanin dye for use in conjugation to biomolecules and antibodies

dye Antibody, Biotin conjugated
1-CSB-PA772049LD01DIL	Cusabio	EUR 380.40 EUR 402.00	100ug 50ug
Description: A polyclonal antibody against dye. Recognizes dye from Zebrafish. This antibody is Biotin conjugated. Tested in the following application: ELISA

INT dye (Iodonitrotetrazolium chloride)
IB0280	Bio Basic	250mg	EUR 75.66

BCIP-NBT Dye for IHC
G447	ABM	250 ml	EUR 304.8

1X4 Colorâ„¢ V405 Dye Set
8000-1Set	AAT Bioquest	1 Set	EUR 1019
Description: 1X4 Colorâ„¢ V405 Dye Set provides a set of highly fluorescent dyes for multicolor applications.

1X4 Colorâ„¢ B488 Dye Set
8005-1Set	AAT Bioquest	1 Set	EUR 1019
Description: 1X4 Colorâ„¢ B488 Dye Set provides a set of highly fluorescent dyes for multicolor applications.

iVDye 6X Gel Loading Dye
V1004-001	GenDepot	1ml	EUR 87.6

iVDye 6X Gel Loading Dye
V1004-010	GenDepot	10x1ml	EUR 213.6

iVDye 6X Gel Loading Dye
V1004-020	GenDepot	20x1ml	EUR 258

PCR SuperMix for PAGE (+dye)
20-abx098004	Abbexa	EUR 493.20 EUR 226.80 EUR 309.60	15 ml 1 ml 5 ml

Powerpol 2xPCR mix with dye
RK20719	Abclonal	1mL	Ask for price

G50 Dye Terminator Removal Kit
FYG603-50P	Yeastern Biotech	50 Preps	Ask for price

PEGylated Liposomal Rhodamine B Dye
PHC001RB	ProFoldin	1 ml	EUR 140.62
Description: This product includes 1 ml of 0.1 mM Rhodamine B encapsulated in liposome composed of HSPC:DSPE-PEG2000:Cholesterol (w/w 3:1:1).

1X4 Color™ V405 Dye Set
8000	AAT Bioquest	1 Set	EUR 1019

1X4 Color™ B488 Dye Set
8005	AAT Bioquest	1 Set	EUR 1019

PEGylated Liposomal Acridine Orange Dye
PHC001AO	ProFoldin	1 ml	EUR 140.62
Description: This product includes 1 ml of 0.1 mM acridine orange encapsulated in liposome composed of HSPC:DSPE-PEG2000:Cholesterol (w/w 3:1:1).

2 × Taq Master Mix (Dye Plus)
P112-01	Vazyme	5 ml	EUR 140.4

2 × Taq Master Mix (Dye Plus)
P112-02	Vazyme	15 ml	EUR 175.2

2 × Taq Master Mix (Dye Plus)
P112-03	Vazyme	50 ml	EUR 283.2

ROX Reference Dye (25 µM; 50x)
F153	GeneOn	1 ml	EUR 73.2

ROX Reference Dye (25 µM; 50x)
F153L	GeneOn	5x1 ml	EUR 206.4

Fast Red Violet Dye for IHC
G446	ABM	250 ml	EUR 410.4

FALCON® DIIC12(3) FLUORESCENT DYE, 100MG
354218	CORNING	1/pk	EUR 381.6
Description: Permeable Support - DL; Coated Transwells - DL

Green-DNA Dye, ready to use
DT81414	Bio Basic	1.5ml, 1.5ml	EUR 153.96

Load 6x Loading Dye Orange G
BA01402	Bioatlas	6x1ml	EUR 96
Description: High purity buffer for various PCR applications.

2 × AceTaq Master Mix (Dye Plus)
P412-01	Vazyme	1 ml	EUR 142.8

2 × AceTaq Master Mix (Dye Plus)
P412-02	Vazyme	5 ml	EUR 189.6

2 × AceTaq Master Mix (Dye Plus)
P412-03	Vazyme	15 ml	EUR 314.4

2X PCR Taq MasterMix with dye
G013-dye	ABM	5.0 ml (200 Rxns)	EUR 104.4

2X PCR Taq MasterMix with dye
G900-dye	ABM	25.0 ml (1000 Rxns)	EUR 246

Top Green qPCR SuperMix (+Dye II)
20-abx098890	Abbexa	EUR 994.80 EUR 260.40 EUR 1362.00 EUR 493.20	15 ml 1 ml 25 ml 5 ml

Tip Green qPCR SuperMix (+Dye II)
20-abx098891	Abbexa	EUR 1161.60 EUR 276.00 EUR 1596.00 EUR 560.40	15 ml 1 ml 25 ml 5 ml

Kodaq 2X PCR MasterMix with dye
G497-dye	ABM	5.0 ml (200 Rxns)	EUR 189.6

1.2ML Triple Dye Loading Buffer (6X)
NAT1192	Scientific Laboratory Supplies	1.2ML	EUR 39.6

2X PCR TaqFast MasterMix with dye
G280-dye	ABM	5.0 ml (200 Rxns)	EUR 160.8

2X PCR HotStart MasterMix with dye
G906-dye	ABM	5.0 ml (200 Rxns)	EUR 189.6

Amersham Cy5 mono Reactive Dye Pack
PA25001	Scientific Laboratory Supplies	EACH	EUR 343.2

TaqProbe 5X qPCR MasterMix-No Dye
MasterMix-5PS	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 255.6

TaqProbe 2X qPCR MasterMix-No Dye
MasterMix-PS	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

Load 6x Loading Dye Bromophenol Blue
BA01401	Bioatlas	6x1ml	EUR 96
Description: High purity buffer for various PCR applications.

KiloGreen 2X qPCR MasterMix-No Dye
MasterMix-KS	ABM	4 x 1.25 ml - 500 reactions (20 ul)	EUR 168

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye
MB1004-100Reactions	BosterBio	100 Reactions	EUR 392.4

VIC Dye qPCR Calibration Solution *10,000X*
67030	AAT Bioquest	100 ul	EUR 301

NED Dye qPCR Calibration Solution *10,000X*
67031	AAT Bioquest	100 ul	EUR 301

VIC Dye qPCR Calibration Solution *10,000X*
67030-100ul	AAT Bioquest	100 ul	EUR 301
Description: Real-time thermal cyclers require calibration to generate a spectral profile for the fluorescence signal.

NED Dye qPCR Calibration Solution *10,000X*
67031-100ul	AAT Bioquest	100 ul	EUR 301
Description: Real-time thermal cyclers require calibration to generate a spectral profile for the fluorescence signal.

FAM Dye qPCR Calibration Solution *10,000X*
67032-100ul	AAT Bioquest	100 ul	EUR 301
Description: The AAT Bioquest FAM dye qPCR Calibration Solution supplied as a 10,000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudioâ„¢, and ViiAâ„¢ 7 systems.

Cy5 Dye qPCR Calibration Solution *10,000X*
67033-100ul	AAT Bioquest	100 ul	EUR 301
Description: The AAT Bioquest Cy5 dye qPCR Calibration Solution supplied as a 10,000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudioâ„¢, and ViiAâ„¢ 7 systems.

ROX Dye qPCR Calibration Solution *10,000X*
67034-100ul	AAT Bioquest	100 ul	EUR 301
Description: The AAT Bioquest ROX dye qPCR Calibration Solution supplied as a 10,000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudioâ„¢, and ViiAâ„¢ 7 systems.

BrightGreen 5X qPCR MasterMix-No Dye
MasterMix-5S	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 255.6

BrightGreen 2X qPCR MasterMix-No Dye
MasterMix-S	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

BrightGreen 2X qPCR MasterMix-No Dye
MasterMix-S-XL	ABM	16 x 1.25 ml for 2000 reactions (20 ul)	EUR 451.2

100bp plus Ladder (no loading dye, 100-1500 bp)
306-005	GeneOn	50µg/250µl	EUR 60

100bp plus Ladder (no loading dye, 100-1500 bp)
306-025	GeneOn	5x50 µg	EUR 144

Cell Meterâ„¢ BX520 fixable viability dye
22510-200Tests	AAT Bioquest	200 Tests	EUR 211
Description: Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations.

Cell Meterâ„¢ BX590 fixable viability dye
22514-200Tests	AAT Bioquest	200 Tests	EUR 211
Description: Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations.

Cell Meterâ„¢ BX650 fixable viability dye
22520-200Tests	AAT Bioquest	200 Tests	EUR 211
Description: Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations.

Cell Meterâ„¢ IX830 fixable viability dye
22529-200Tests	AAT Bioquest	200 Tests	EUR 211
Description: Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations.

Cell Meterâ„¢ RX660 fixable viability dye
22530-200Tests	AAT Bioquest	200 Tests	EUR 211
Description: Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations.

Cell Meterâ„¢ RX700 fixable viability dye
22532-200Tests	AAT Bioquest	200 Tests	EUR 211
Description: Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations.

Cell Meterâ„¢ RX780 fixable viability dye
22536-200Tests	AAT Bioquest	200 Tests	EUR 211
Description: Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations.

Cell Meterâ„¢ VX450 fixable viability dye
22540-200Tests	AAT Bioquest	200 Tests	EUR 211
Description: Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations.

Cell Meterâ„¢ VX500 fixable viability dye
22542-200Tests	AAT Bioquest	200 Tests	EUR 211
Description: Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations.

Taq PCR Master Mix (2X, Red Dye)
BS9297	Bio Basic	1ml	EUR 101.76

Taq PCR Master Mix (2X, Red Dye)
BS9298	Bio Basic	5ml	EUR 227.04

BrightGreen miRNA qPCR MasterMix-No Dye
MasterMix-mS	ABM	4 x 1.25 ml for 500 reactions	EUR 181.2

Taq PCR Master Mix (2X, Blue Dye)
BS9295	Bio Basic	1ml	EUR 101.76

Taq PCR Master Mix (2X, Blue Dye)
BS9296	Bio Basic	5ml	EUR 227.04

2×Taq PCR Master Mix(with dye)
K1034-1	ApexBio	1 ml	EUR 87.6

2×Taq PCR Master Mix(with dye)
K1034-100	ApexBio	100×1 ml	EUR 950.4

2×Taq PCR Master Mix(with dye)
K1034-20	ApexBio	20×1 ml	EUR 338.4

2×Taq PCR Master Mix(with dye)
K1034-5	ApexBio	5×1 ml	EUR 142.8

2×Taq PCR Master Mix(with dye)
K1034-50	ApexBio	50×1 ml	EUR 616.8

2 × Taq Plus Master Mix (Dye Plus)
P212-01	Vazyme	5 ml	EUR 159.6

2 × Taq Plus Master Mix (Dye Plus)
P212-02	Vazyme	15 ml	EUR 231.6

2 × Taq Plus Master Mix (Dye Plus)
P212-03	Vazyme	50 ml	EUR 466.8

Comb 1 sample 1 dye 1mm thick
FMMS11	Scientific Laboratory Supplies	EACH	EUR 37.2

Comb 1 sample 1 dye 1.5mm thick
FMMS115	Scientific Laboratory Supplies	EACH	EUR 34.8

ROX reference dye for qPCR, 1 mL
41110	Lumiprobe	1 mL	EUR 62.4

Eco-Red-DNA Dye, ready to use
DT81415	Bio Basic	1ml	EUR 122.64

One-Step RT-PCR Kit with dye
G174-dye	ABM	100 x 50 ul reactions	EUR 279.6

2 × Phanta Max Master Mix (Dye Plus)
P525-01	Vazyme	1 ml	EUR 174

2 × Phanta Max Master Mix (Dye Plus)
P525-02	Vazyme	5 ml	EUR 322.8

2 × Phanta Max Master Mix (Dye Plus)
P525-03	Vazyme	15 ml	EUR 690

Eco-White-DNA Dye, ready to use
DT81417	Bio Basic	1ml	EUR 122.64

2 × Vazyme LAmp Master Mix (Dye Plus)
P312-01	Vazyme	1 ml	EUR 165.6

2 × Vazyme LAmp Master Mix (Dye Plus)
P312-02	Vazyme	5 ml	EUR 254.4

2 × Vazyme LAmp Master Mix (Dye Plus)
P312-03	Vazyme	15 ml	EUR 481.2

2X PCR Taq Plus MasterMix with dye
G014-dye	ABM	5.0 ml (200 Rxns)	EUR 127.2

2X PCR Taq Plus MasterMix with dye
G901-dye	ABM	25.0 ml (1000 Rxns)	EUR 358.8