Experimental Study of Shenfu Injection on the Prevention and Treatment of Paclitaxel Chemotherapy DRG Neuron Injury.
The function of this paper is investigating the impact and mechanism of Shenfu injection (a Traditional Chinese Medicine injection type) on prevention and therapy of paclitaxel chemotherapy in peripheral nerve harm.MethodsWistar rat dorsal root ganglion cells had been cultured in vitro and divided into teams of MOCK, PT, PT + LD, and PT + HD.
Each group was cultured at a complete serum focus of 10%, together with 10% clean serum in the MOCK group, 0.73 (IC30) μmol/L paclitaxel + 10% clean serum in the PT group, and 10% and 5% drug-containing serum and equal quantity of paclitaxel had been added into the high- and low-dosage teams, respectively.
After culturing for 24 hours, the following checks had been carried out: (1) cell proliferation detected by utilizing CCK-8 and a microplate reader; (2) axon size detected by mobile immunostaining and detection evaluation on antibody β-tubulin III; and (3) modifications in mitochondrial membrane potential by analyzing immunofluorescence staining with JC-1 probe.
Results(1) Cell proliferation: OD values of the MOCK group and PT group had been 0.43 ± 0.02 and 0.25 ± 0.03, respectively (P < 0.05), whereas OD values of teams PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, larger than group PT (P < 0.05), whereas OD values of teams PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, larger than group PT (μmol/L paclitaxel + 10% clean serum in the PT group, and 10% and 5% drug-containing serum and equal quantity of paclitaxel had been added into the high- and low-dosage teams, respectively.
After culturing for 24 hours, the following checks had been carried out: (1) cell proliferation detected by utilizing CCK-8 and a microplate reader; (2) axon size detected by mobile immunostaining and detection evaluation on antibody μmol/L paclitaxel + 10% clean serum in the PT group, and 10% and 5% drug-containing serum and equal quantity of paclitaxel had been added into the high- and low-dosage teams, respectively.
MR9600 and MW9600, does not include 8 or 12 channel manifold for MW9600. |
|||
MR9600-BND | Benchmark Scientific | each | EUR 10294.85 |
T(Testosterone) ELISA Kit |
|||
EU0400 | FN Test | 96T | EUR 571.5 |
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: General;Sensitivity: 0.188 ng/ml |
Human Testosterone ELISA Kit |
|||
EH4850 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens ;Sensitivity: 0.188 ng/ml |
Mouse Testosterone ELISA Kit |
|||
EM1850 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Mus ;Sensitivity: 0.188 ng/ml |
Rat T(Testosterone) ELISA Kit |
|||
ER1462 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Rattus;Sensitivity: 0.188 ng/ml |
Bovine T(Testosterone) ELISA Kit |
|||
EB0049 | FN Test | 96T | EUR 681.12 |
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Cattle;Sensitivity: 0.188 ng/ml |
Human SPOCK2(Testican-2) ELISA Kit |
|||
EH2271 | FN Test | 96T | EUR 681.12 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
MR9600 and MW9600, does not include 8 or 12 channel manifold for MW9600. 230V |
|||
MR9600-BND-E | Benchmark Scientific | each | EUR 10294.85 |
Human TEX101(testis expressed 101) ELISA Kit |
|||
EH12903 | FN Test | 96T | EUR 681.12 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens ;Sensitivity: 0.188 ng/ml |
Human F-TESTO(Free Testoterone) ELISA Kit |
|||
EH3092 | FN Test | 96T | EUR 571.5 |
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml |
Test for Enteropathogenic.C |
|||
MIC6774 | Scientific Laboratory Supplies | PK50 | EUR 215.46 |
Human PDHA2(Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial) ELISA Kit |
|||
EH1149 | FN Test | 96T | EUR 681.12 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
Rat Pdha2(Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial) ELISA Kit |
|||
ER0417 | FN Test | 96T | EUR 681.12 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 46.9pg/ml |
Cysticercus (Cyt) antibody ELISA test kit for Livestock |
|||
LSY-30021 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Mouse LAT(Linker for activation of T cell) ELISA Kit |
|||
EM1183 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml |
Human LAT(Linker For Activation of T-cell) ELISA Kit |
|||
EH3288 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
Test for Identify Legionella.p |
|||
MIC6772 | Scientific Laboratory Supplies | PK50 | EUR 266.76 |
Human CD32(Receptor II for the Fc Fragment of IgG) ELISA Kit |
|||
EH0083 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 28.125pg/ml |
Human DR-70TM(Tumor Marker DR-70 For Lung Cancer) ELISA Kit |
|||
EH2961 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ug/ml |
Rat sRAGE(Solube Receptor for Advanced Glycation End product) ELISA Kit |
|||
ER1853 | FN Test | 96T | EUR 681.12 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 46.875pg/ml |
Human sRAGE(Solube Receptor for Advanced Glycation End product) ELISA Kit |
|||
EH0408 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
Test for Identif of Staph.a |
|||
MIC6766 | Scientific Laboratory Supplies | PK100 | EUR 91.2 |
Test for Identif of Staph.a |
|||
MIC6768 | Scientific Laboratory Supplies | PK500 | EUR 311.22 |
Human CD23(Receptor II for the Fc Region of Immunoglobulin E) ELISA Kit |
|||
EH0079 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
Pyrazinamidase Test Kit for |
|||
K045-KT | EWC Diagnostics | 1 unit | EUR 18.31 |
Description: Pyrazinamidase Test Kit for |
Human FcγR I(Receptor I for the Fc Region of Immunoglobulin G) ELISA Kit |
|||
EH3046 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.469 ng/ml |
Test for Identifi of Listeria |
|||
MIC6778 | Scientific Laboratory Supplies | PK50 | EUR 119.7 |
Human esRAGE(Endogenous secretory Receptor for Advanced Glycation End product) ELISA Kit |
|||
EH2885 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.4pg/ml |
Test for Identi of Clostridium.d |
|||
MIC6762 | Scientific Laboratory Supplies | PK50 | EUR 183.54 |
Test for Identi of Salmonellae |
|||
MIC6764 | Scientific Laboratory Supplies | PK50 | EUR 139.08 |
Test for Identifying E.coli O157 |
|||
MIC6770 | Scientific Laboratory Supplies | PK50 | EUR 78.66 |
Quinolones(QNS) ELISA Test Kit Fluoroquinolone ELISA Test Kit |
|||
LSY-10016 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
ELISA test-systems |
|||
WM-4 | Awareness Technology | 1 unit | Ask for price |
Rat MTL5(Tesmin) ELISA Kit |
|||
ER1521 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml |
1-hour Dog meat adulteration ELISA test (for beef or pig meat; 96 tests) |
|||
DMT-96 | Alpha Diagnostics | 1 kit | EUR 1000.8 |
1-hour Rat meat adulteration ELISA test (for beef or pig meat; 96 tests) |
|||
RMT-96 | Alpha Diagnostics | 1 kit | EUR 1000.8 |
Test for Strep groups ABCDF&G |
|||
MIC6776 | Scientific Laboratory Supplies | PK50 | EUR 167.58 |
Mini Incubator for Test Kit |
|||
M1005 | Ring Biotechnology Co | 1set | EUR 100 |
Description: Instrument |
NOVATest Antigen Rapid Test Kit (For Single Use) (NOVA Test) |
|||
nCov-500-01 | Atlas Link Technology | 1T | EUR 162 |
Nitrate Reduction Test Kit for |
|||
K043-KT | EWC Diagnostics | 1 unit | EUR 30.49 |
Description: Nitrate Reduction Test Kit for |
1-hour Beef/Cow meat adulteration ELISA test (for beef or pig meat; 96 tests) |
|||
BMT-96 | Alpha Diagnostics | 1 kit | EUR 1000.8 |
Test Tube Rack Holder (for 50 Ml Test Tube Racks) - EACH |
|||
480169 | Scientific Laboratory Supplies | EACH | EUR 66.15 |
Tylosin ELISA Test Kit |
|||
LSY-10020 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Biochemical Test Kit for Shigella |
|||
K140-10KT | EWC Diagnostics | 1 unit | EUR 130.52 |
Description: Biochemical Test Kit for Shigella |
Biochemical Test Kit for Shigella |
|||
K140-5KT | EWC Diagnostics | 1 unit | EUR 72.53 |
Description: Biochemical Test Kit for Shigella |
1-hour Horse/Donkey meat adulteration ELISA test (for beef or pig meat; 96 tests) |
|||
HMT-96 | Alpha Diagnostics | 1 Kit | EUR 1000.8 |
Neomycin ELISA test kit |
|||
LSY-10005 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Diazepam ELISA Test Kit |
|||
LSY-10021 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Melamine ELISA test kit |
|||
LSY-10026 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Colistin ELISA Test Kit |
|||
LSY-10056 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Catalase Test Kit for Mycobacteria |
|||
K044-KT | EWC Diagnostics | 1 unit | EUR 30.93 |
Description: Catalase Test Kit for Mycobacteria |
Ofloxacin ELISA Test Kit |
|||
LSY-10064 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Cefalexin ELISA Test Kit |
|||
LSY-10067 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Biochemical Test Kit for Salmonella |
|||
K141-10KT | EWC Diagnostics | 1 unit | EUR 25.63 |
Description: Biochemical Test Kit for Salmonella |
Biochemical Test Kit for Salmonella |
|||
K141-5KT | EWC Diagnostics | 1 unit | EUR 14.23 |
Description: Biochemical Test Kit for Salmonella |
Salbutamol ELISA Test Kit |
|||
LSY-10014 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Ampicillin ELISA Test Kit |
|||
LSY-10018 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Gentamicin ELISA Test Kit |
|||
LSY-10023 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Olaquindox ELISA Test Kit |
|||
LSY-10025 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Lincomycin ELISA Test Kit |
|||
LSY-10045 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Amantadine ELISA test kit |
|||
LSY-10052 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Tilmicosin ELISA test kit |
|||
LSY-10053 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Avermectin ELISA test kit |
|||
LSY-10060 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Influenza A Virus antibody ELISA Test Kit for pig, birds, dog, horse |
|||
LSY-30043 | Shenzhen Lvshiyuan Biotechnology | 192 well/kit | Ask for price |
1-hour Pig meat adulteration ELISA test (for beef or pig meat; 96 tests), Quantitative |
|||
PMT-96 | Alpha Diagnostics | 1 kit | EUR 1000.8 |
Doxycycline ELISA Test Kit |
|||
DEIARN99048 | Creative Diagnostics | 96T | EUR 1365 |
Description: feed, tissue(meat,liver and kidney), milk, serum,plasma and urine |
Florfenicol ELISA Test Kit |
|||
LSY-10008 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Clenbuterol ELISA Test Kit |
|||
LSY-10012 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Ractopamine ELISA Test Kit |
|||
LSY-10013 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Zearalenone ELISA test kit |
|||
LSY-10030 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Doxycycline ELISA test kit |
|||
LSY-10051 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Amoxicillin ELISA Test Kit |
|||
LSY-10054 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Nicarbazine ELISA test kit |
|||
LSY-10063 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Enrofloxacin ELISA Test Kit |
|||
LSY-10017 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Streptomycin ELISA Test Kit |
|||
LSY-10024 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Streptomycin ELISA Test Kit |
|||
LSY-10024-2 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Erythromycin ELISA test kit |
|||
LSY-10050 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Azithromycin ELISA Test Kit |
|||
LSY-10057 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Trimethoprim ELISA Test Kit |
|||
LSY-10065 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Syphilis Antibody ELISA test |
|||
100 | Biobase | 96T/Box | Ask for price |
Description: ELISA based test for quantitative detection of Syphilis Antibody |
Dexamethasone ELISA Test Kit |
|||
LSY-10042 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Metronidazole elisa test kit |
|||
LSY-10048 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Dimitridazole ELISA Test Kit |
|||
LSY-10061 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Ciprofloxacin ELISA Test Kit |
|||
LSY-10062 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Cyproheptadine ELISA Test Kit |
|||
LSY-10041 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Oxytetracyline ELISA Test Kit |
|||
LSY-10044 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Test tube tray for OLS26 - EACH |
|||
BAT1066 | Scientific Laboratory Supplies | EACH | EUR 321.3 |
Test tube tray for LSB12 - EACH |
|||
BAT1068 | Scientific Laboratory Supplies | EACH | EUR 305.1 |
Test tube tray for LSB18 - EACH |
|||
BAT1070 | Scientific Laboratory Supplies | EACH | EUR 321.3 |
Nitroimidazoles ELISA Test Kit |
|||
LSY-10047 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Peste des petits ruminants virus (PPRV) antibody ELISA test kit for ovine |
|||
LSY-30035 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Barritt Reagent A (for VP test) |
|||
R029-100ML | EWC Diagnostics | 1 unit | EUR 6.48 |
Description: Barritt Reagent A (for VP test) |
Barritt Reagent B (for VP test) |
|||
R030-100ML | EWC Diagnostics | 1 unit | EUR 2.89 |
Description: Barritt Reagent B (for VP test) |
Chlortetracyline ELISA test kit |
|||
LSY-10049 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
FMDV NSP Antibody 3ABC ELISA test kit for cattle, sheep, goat and pig |
|||
LSY-30040 | Shenzhen Lvshiyuan Biotechnology | 96*2 well/kit | Ask for price |
12 Test System for Neg Bacteria |
|||
MIC6708 | Scientific Laboratory Supplies | PK24 | EUR 110.58 |
Kinesis Siltite Micron union Re order 0.8-0.8 does not include ferrules - EACH |
|||
CHR1933 | Scientific Laboratory Supplies | EACH | EUR 218.7 |
Zinc Metal filing for Arsenic Test |
|||
GRM10955-500G | EWC Diagnostics | 1 unit | EUR 30.25 |
Description: Zinc Metal filing for Arsenic Test |
Aflatoxin M1 ELISA test kit |
|||
LSY-10032 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Fumonisin B1 ELISA test kit |
|||
LSY-10033 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Aflatoxins B1 ELISA test kit |
|||
LSY-10028 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Ochratoxin A ELISA test kit |
|||
LSY-10034 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Test kit for Potential Antioxidant(PAO) |
|||
KPA-050 | Jaica | 1 Unit | EUR 307.38 |
Description: Antioxidantassay |
Nitrofuran (AOZ) ELISA Test Kit |
|||
LSY-10002 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Nitrofuran (AHD) ELISA Test Kit |
|||
LSY-10003 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Nitrofuran (SEM) ELISA Test Kit |
|||
LSY-10004 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Biochemical Test Kit for Vibrio cholera |
|||
K138-10KT | EWC Diagnostics | 1 unit | EUR 94.6 |
Description: Biochemical Test Kit for Vibrio cholera |
Biochemical Test Kit for Vibrio cholera |
|||
K138-5KT | EWC Diagnostics | 1 unit | EUR 49.85 |
Description: Biochemical Test Kit for Vibrio cholera |
Zearalenone(ZEN)ELISA Test Kit |
|||
EK74024 | AFG Bioscience LLC | 96 Wells | EUR 0.1 |
Nitrofuran (AMOZ) ELISA Test Kit |
|||
LSY-10001 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
SS test tube rack for 42 tubes |
|||
BAT8160 | Scientific Laboratory Supplies | EACH | EUR 110.25 |
Biochemical Test Kit for Vibrio parahaem |
|||
K139-10KT | EWC Diagnostics | 1 unit | EUR 94.92 |
Description: Biochemical Test Kit for Vibrio parahaem |
Biochemical Test Kit for Vibrio parahaem |
|||
K139-5KT | EWC Diagnostics | 1 unit | EUR 50 |
Description: Biochemical Test Kit for Vibrio parahaem |
Recombinant human Testin |
|||
P2531 | FN Test | 100ug | Ask for price |
Description: Recombinant protein for human Testin |
HiRapid™Formalin Test Kit (For Fish) |
|||
K137-1KT | EWC Diagnostics | 1 unit | EUR 3.79 |
Description: HiRapid™Formalin Test Kit (For Fish) |
Tetrodotoxin (TTX) ELISA Test Kit |
|||
DEIANJ48 | Creative Diagnostics | 96T | EUR 1950 |
Description: This test kit is for the quantitative detection of Tetrodotoxin in the tissue, liver, fish. |
Total Aflatoxins ELISA test kit |
|||
LSY-10029 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Sulfadiazine(SDZ) ELISA Test Kit |
|||
LSY-10055 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Dengue Antigen for Rapid Test Recombinant |
|||
rAP-5175 | Angio Proteomie | Inquiry | Ask for price |
Tetracyclines(TCs) ELISA Test Kit |
|||
LSY-10006 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
After culturing for 24 hours, the following checks had been carried out: (1) cell proliferation detected by utilizing CCK-8 and a microplate reader; (2) axon size detected by mobile immunostaining and detection evaluation on antibody P < 0.05), whereas OD values of groups PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, larger than group PT (P < 0.05), whereas OD values of teams PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, larger than group PT (.Shenfu injection can forestall the toxicity of DRG neurons induced by paclitaxel, and its mechanism could also be associated to the alleviation of mitochondrial dysfunction.