Experimental Study of Shenfu Injection on the Prevention and Treatment of Paclitaxel Chemotherapy DRG Neuron Injury.

The function of this paper is investigating the impact and mechanism of Shenfu injection (a Traditional Chinese Medicine injection type) on prevention and therapy of paclitaxel chemotherapy in peripheral nerve harm.MethodsWistar rat dorsal root ganglion cells had been cultured in vitro and divided into teams of MOCK, PT, PT + LD, and PT + HD.

Each group was cultured at a complete serum focus of 10%, together with 10% clean serum in the MOCK group, 0.73 (IC30) μmol/L paclitaxel + 10% clean serum in the PT group, and 10% and 5% drug-containing serum and equal quantity of paclitaxel had been added into the high- and low-dosage teams, respectively.

After culturing for 24 hours, the following checks had been carried out: (1) cell proliferation detected by utilizing CCK-8 and a microplate reader; (2) axon size detected by mobile immunostaining and detection evaluation on antibody β-tubulin III; and (3) modifications in mitochondrial membrane potential by analyzing immunofluorescence staining with JC-1 probe.

Results(1) Cell proliferation: OD values of the MOCK group and PT group had been 0.43 ± 0.02 and 0.25 ± 0.03, respectively (P < 0.05), whereas OD values of teams PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, larger than group PT (P < 0.05), whereas OD values of teams PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, larger than group PT (μmol/L paclitaxel + 10% clean serum in the PT group, and 10% and 5% drug-containing serum and equal quantity of paclitaxel had been added into the high- and low-dosage teams, respectively.

After culturing for 24 hours, the following checks had been carried out: (1) cell proliferation detected by utilizing CCK-8 and a microplate reader; (2) axon size detected by mobile immunostaining and detection evaluation on antibody μmol/L paclitaxel + 10% clean serum in the PT group, and 10% and 5% drug-containing serum and equal quantity of paclitaxel had been added into the high- and low-dosage teams, respectively.

Test for Identify Legionella.p
MIC6772	Scientific Laboratory Supplies	PK50	EUR 266.76

Human TES(Testin) ELISA Kit
EH1738	FN Test	96T	EUR 681.12
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 0.375 ng/ml

T(Testosterone) ELISA Kit
EU0400	FN Test	96T	EUR 571.5
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: General;Sensitivity: 0.188 ng/ml

Mouse Testosterone ELISA Kit
EM1850	FN Test	96T	EUR 628.92
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Mus ;Sensitivity: 0.188 ng/ml

Human Testosterone ELISA Kit
EH4850	FN Test	96T	EUR 628.92
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens ;Sensitivity: 0.188 ng/ml

Rat T(Testosterone) ELISA Kit
ER1462	FN Test	96T	EUR 628.92
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Rattus;Sensitivity: 0.188 ng/ml

Test for Identif of Staph.a
MIC6766	Scientific Laboratory Supplies	PK100	EUR 91.2

Test for Identif of Staph.a
MIC6768	Scientific Laboratory Supplies	PK500	EUR 311.22

Bovine T(Testosterone) ELISA Kit
EB0049	FN Test	96T	EUR 681.12
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Cattle;Sensitivity: 0.188 ng/ml

Human SPOCK2(Testican-2) ELISA Kit
EH2271	FN Test	96T	EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Test for Identifi of Listeria
MIC6778	Scientific Laboratory Supplies	PK50	EUR 119.7

Test for Identi of Clostridium.d
MIC6762	Scientific Laboratory Supplies	PK50	EUR 183.54

Test for Identi of Salmonellae
MIC6764	Scientific Laboratory Supplies	PK50	EUR 139.08

Test for Identifying E.coli O157
MIC6770	Scientific Laboratory Supplies	PK50	EUR 78.66

TESTO (Testosterone) ELISA test
19	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of O (osterone)

Human TEX101(testis expressed 101) ELISA Kit
EH12903	FN Test	96T	EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens ;Sensitivity: 0.188 ng/ml

Human F-TESTO(Free Testoterone) ELISA Kit
EH3092	FN Test	96T	EUR 571.5
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

Test Tube Rack Holder (for 50 Ml Test Tube Racks)
480169	Scientific Laboratory Supplies	EACH	EUR 58.8

Test tube tray for OLS26
BAT1066	Scientific Laboratory Supplies	EACH	EUR 285.6

Test tube tray for LSB12
BAT1068	Scientific Laboratory Supplies	EACH	EUR 271.2

Test tube tray for LSB18
BAT1070	Scientific Laboratory Supplies	EACH	EUR 285.6

Test for Strep groups ABCDF&G
MIC6776	Scientific Laboratory Supplies	PK50	EUR 167.58

NOVATest Antigen Rapid Test Kit (For Single Use) (NOVA Test)
nCov-500-01	Atlas Link Technology	1T	EUR 162

CA72-4 ELISA test
24	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of CA72-4

PreS2-Ag ELISA test
96	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of PreS2-Ag

1-hour Dog meat adulteration ELISA test (for beef or pig meat; 96 tests)
DMT-96	Alpha Diagnostics	1 kit	EUR 1000.8

1-hour Rat meat adulteration ELISA test (for beef or pig meat; 96 tests)
RMT-96	Alpha Diagnostics	1 kit	EUR 1000.8

Rat Pdha2(Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial) ELISA Kit
ER0417	FN Test	96T	EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 46.9pg/ml

Human PDHA2(Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial) ELISA Kit
EH1149	FN Test	96T	EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

T4 (Thyroxine) ELISA test
16	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of T4 (Thyroxine)

E2 (Estradiol) ELISA test
5	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of E2 (Estradiol)

PRL (Prolactin) ELISA test
4	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of PRL (Prolactin)

FERR ( Ferritin) ELISA test
7	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of FERR ( Ferritin)

HBcAb Sandwich ELISA test
95	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of HBcAb Sandwich

TSH (Thyrotrophin) ELISA test
14	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of TSH (Thyrotrophin)

Pipette for Card Test 70mm 1ml
BSV150	Scientific Laboratory Supplies	PK10000	EUR 302.4

Syphilis Antibody ELISA test
100	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of Syphilis Antibody

PROG (Progesterone) ELISA test
20	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of PROG (Progesterone)

12 Test System for Neg Bacteria
MIC6708	Scientific Laboratory Supplies	PK24	EUR 110.58

1-hour Beef/Cow meat adulteration ELISA test (for beef or pig meat; 96 tests)
BMT-96	Alpha Diagnostics	1 kit	EUR 1000.8

T3 (Triiodothyronine) ELISA test
15	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of T3 (Triiodothyronine)

1-hour Horse/Donkey meat adulteration ELISA test (for beef or pig meat; 96 tests)
HMT-96	Alpha Diagnostics	1 Kit	EUR 1000.8

Kinesis Siltite Micron union Re order 0.8-0.8 does not include ferrules
CHR1933	Scientific Laboratory Supplies	EACH	EUR 194.4

Mouse LAT(Linker for activation of T cell) ELISA Kit
EM1183	FN Test	96T	EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml

1-hour Pig meat adulteration ELISA test (for beef or pig meat; 96 tests), Quantitative
PMT-96	Alpha Diagnostics	1 kit	EUR 1000.8

Human LAT(Linker For Activation of T-cell) ELISA Kit
EH3288	FN Test	96T	EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

SS test tube rack for 30 tubes
BAT8158	Scientific Laboratory Supplies	EACH	EUR 126

SS test tube rack for 42 tubes
BAT8159	Scientific Laboratory Supplies	EACH	EUR 130.8

SS test tube rack for 42 tubes
BAT8160	Scientific Laboratory Supplies	EACH	EUR 126

HAV antibody IgM ELISA test
75	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of HAV antibody IgM

HEV antibody IgM ELISA test
92	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of HEV antibody IgM

QUANTOFIX ASCORBIC-ACID TEST STICKS FOR
Z166340-100EA	Scientific Laboratory Supplies	EACH	EUR 54

FT4 (Free thyroxine) ELISA test
18	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of FT4 (Free thyroxine)

CEA (Cancer antigen) ELISA test
9	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of CEA (Cancer antigen)

CA50 (Cancer antigen) ELISA test
10	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of CA50 (Cancer antigen)

CA199 (Cancer antigen) ELISA test
13	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of CA199 (Cancer antigen)

CA153 (Cancer antigen) ELISA test
12	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of CA153 (Cancer antigen)

CA125 (Cancer antigen) ELISA test
11	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of CA125 (Cancer antigen)

Tetrodotoxin (TTX) ELISA Test Kit
DEIANJ48	Creative Diagnostics	96T	EUR 1950
Description: This test kit is for the quantitative detection of Tetrodotoxin in the tissue, liver, fish.

plastic test tube rack for 60 tubes
BAT8155	Scientific Laboratory Supplies	EACH	EUR 78

plastic test tube rack for 90 tubes
BAT8156	Scientific Laboratory Supplies	EACH	EUR 78

plastic test tube rack for 21 tubes
BAT8157	Scientific Laboratory Supplies	EACH	EUR 78

Human CD32(Receptor II for the Fc Fragment of IgG) ELISA Kit
EH0083	FN Test	96T	EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 28.125pg/ml

AFP (Alpha fetoprotein) ELISA test
6	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of AFP (Alpha fetoprotein)

LH (Luteinizing Hormone) ELISA test
2	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of LH (Luteinizing Hormone)

Human DR-70TM(Tumor Marker DR-70 For Lung Cancer) ELISA Kit
EH2961	FN Test	96T	EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ug/ml

Test Tube Rack Holder (for 15 Ml Test Tube Racks) Fits I-5330 Platform
480168	Scientific Laboratory Supplies	EACH	EUR 58.8

Rat sRAGE(Solube Receptor for Advanced Glycation End product) ELISA Kit
ER1853	FN Test	96T	EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 46.875pg/ml

Human sRAGE(Solube Receptor for Advanced Glycation End product) ELISA Kit
EH0408	FN Test	96T	EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

FT3 (Free triiodothyronine) ELISA test
17	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of FT3 (Free triiodothyronine)

Test kit for Potential Anti Oxidant (PAO)
LF-EK90001	Abfrontier	1x96T	EUR 1050

Test Tube rack for 40 x 0.5mL tubes
BAT3018	Scientific Laboratory Supplies	EACH	EUR 136.8

Human CD23(Receptor II for the Fc Region of Immunoglobulin E) ELISA Kit
EH0079	FN Test	96T	EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Test Tube rack for 41 x 15mL tubes
BAT3070	Scientific Laboratory Supplies	EACH	EUR 145.2

Test Tube rack for 15 x 50mL tubes
BAT3072	Scientific Laboratory Supplies	EACH	EUR 145.2

Test Tube rack for 76 x 15mL tubes
BAT3074	Scientific Laboratory Supplies	EACH	EUR 289.2

Test Tube rack for 30 x 50mL tubes
BAT3076	Scientific Laboratory Supplies	EACH	EUR 289.2

CYFRA211 (Keratin 21-1) ELISA test
23	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of CYFRA211 (Keratin 21-1)

HIV 1+2 Ab CE ELISA test
97	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of HIV 1+2 Ab CE

HIV 1+2 Ag/Ab ELISA test
98	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of HIV 1+2 Ag/Ab

IgE (Immune globulin E) ELISA test
21	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of IgE (Immune globulin)

SS test tube rack for 10 Falcon tubes
BAT8161	Scientific Laboratory Supplies	EACH	EUR 126

Human FcγR I(Receptor I for the Fc Region of Immunoglobulin G) ELISA Kit
EH3046	FN Test	96T	EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.469 ng/ml

RV (Rubella Antibody IgM) ELISA test
31	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of RV (Rubella Antibody IgM)

RV (Rubella Antibody IgG) ELISA test
30	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of RV (Rubella Antibody IgG)

Human esRAGE(Endogenous secretory Receptor for Advanced Glycation End product) ELISA Kit
EH2885	FN Test	96T	EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.4pg/ml

Rat MTL5(Tesmin) ELISA Kit
ER1521	FN Test	96T	EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml

ACA Anticardiolipin Ab IgA ELISA test
122	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of ACA Anticardiolipin Ab IgA

IAA (Anti-insulin antibody) ELISA test
25	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of IAA (Anti-insulin antibody)

Adv (Adenovirus Antibody IgM) ELISA test
44	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of Adv (Adenovirus Antibody IgM)

TOX (Toxoplasma Antibody IgM) ELISA test
33	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of TOX (Toxoplasma Antibody IgM)

TOX (Toxoplasma Antibody IgG) ELISA test
32	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of TOX (Toxoplasma Antibody IgG)

Syphilis anti-TP syphilis ELISA test
99	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of Syphilis anti-TP syphilis

Test Tube rack for 41 x 15 ml tubes
B2000-4-T150	Benchmark Scientific	1 each	EUR 109.3

Test Tube rack for 40 x 0.5 ml tubes
B2000-4-T5	Benchmark Scientific	1 PC	EUR 101.9

Test Tube rack for 15 x 50 ml tubes
B2000-4-T500	Benchmark Scientific	1 each	EUR 109.3

Test Tube rack for 76 x 15 ml tubes
B2000-8-T150	Benchmark Scientific	1 each	EUR 219.7

Test Tube rack for 30 x 50 ml tubes
B2000-8-T500	Benchmark Scientific	1 each	EUR 219.7

Test Tube rack for 15 x 50 ml tubes
 -B2000-4-T500	Benchmark Scientific	1 PC	EUR 252.35

Test Tube rack for 41 x 15 ml tubes
  B2000-4-T150	Benchmark Scientific	1 PC	EUR 164.65

Test Tube rack for 76 x 15 ml tubes
  B2000-8-T150	Benchmark Scientific	1 PC	EUR 252.35

Test Tube rack for 30 x 50 ml tubes
  B2000-8-T500	Benchmark Scientific	1 PC	EUR 2995.98

Standard Test Slide for QuadCount, pk of 1
E7500-STS1	Benchmark Scientific	1 each	EUR 280.9

ANA (Antinuclear Antibody IgG) ELISA test
52	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of ANA (Antinuclear Antibody IgG)

Test Tube rack for 40 x 1.5/2.0mL tubes
BAT3048	Scientific Laboratory Supplies	EACH	EUR 128.4

ASA Anti-sperm Ab IgG ELISA test
118	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of ASA Anti-sperm Ab IgG

COX (Coxsackievirus Antibody IgG) ELISA test
140	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of COX (Coxsackievirus Antibody IgG)

COX (Coxsackievirus Antibody IgM) ELISA test
141	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of COX (Coxsackievirus Antibody IgM)

PSA (Prostate-specific antigen) ELISA test
8	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of PSA (Prostate-specific antigen)

Porcine Parvovirus Antibody Elisa Test Kit
767-LSY-30009	Gentaur Genprice	192 Wells/kit	EUR 382

?-HCG (Human chorionic gonadotropin) ELISA test
1	Cusabio	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of ?-HCG (Human chorionic gonadotropin)

HGH (Human chorionic gonadotropin) ELISA test
22	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of HGH (Human chorionic gonadotropin)

CMV (Cytomegalovirus Antibody IgG) ELISA test
28	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of CMV (Cytomegalovirus Antibody IgG)

ACA (Anticardiolipin Antibody IgA) ELISA test
61	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of ACA (Anticardiolipin Antibody IgA)

ACA (Anticardiolipin Antibody IgM) ELISA test
60	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of ACA (Anticardiolipin Antibody IgM)

ACA (Anticardiolipin Antibody IgG) ELISA test
59	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of ACA (Anticardiolipin Antibody IgG)

CMV (Cytomegalovirus Antibody IgM) ELISA test
29	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of CMV (Cytomegalovirus Antibody IgM)

HCV-IgM hepatitis C IgM ELISA test
87	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of HCV-IgM hepatitis C IgM

HDV-IgG hepatitis D IgG ELISA test
88	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of HDV-IgG hepatitis D IgG

HDV-IgM hepatitis D IgM ELISA test
89	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of HDV-IgM hepatitis D IgM

After culturing for 24 hours, the following checks had been carried out: (1) cell proliferation detected by utilizing CCK-8 and a microplate reader; (2) axon size detected by mobile immunostaining and detection evaluation on antibody P < 0.05), whereas OD values of groups PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, larger than group PT (P < 0.05), whereas OD values of teams PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, larger than group PT (.Shenfu injection can forestall the toxicity of DRG neurons induced by paclitaxel, and its mechanism could also be associated to the alleviation of mitochondrial dysfunction.