The goal of this paper is investigating the impact and mechanism of Shenfu injection (a Traditional Chinese Medicine injection type) on prevention and therapy of paclitaxel chemotherapy in peripheral nerve harm.MethodsWistar rat dorsal root ganglion cells had been cultured in vitro and divided into teams of MOCK, PT, PT + LD, and PT + HD.
Each group was cultured at a complete serum focus of 10%, together with 10% clean serum in the MOCK group, 0.73 (IC30) μmol/L paclitaxel + 10% clean serum in the PT group, and 10% and 5% drug-containing serum and equal quantity of paclitaxel had been added into the high- and low-dosage teams, respectively.
After culturing for 24 hours, the following assessments had been carried out: (1) cell proliferation detected through the use of CCK-8 and a microplate reader; (2) axon size detected by mobile immunostaining and detection evaluation on antibody β-tubulin III; and (3) adjustments in mitochondrial membrane potential by analyzing immunofluorescence staining with JC-1 probe.Results(1) Cell proliferation: OD values of the MOCK group and PT group had been 0.43 ± 0.02 and 0.25 ± 0.03, respectively (P < 0.05), whereas OD values of teams PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, greater than group PT (P < 0.05), whereas OD values of teams PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, greater than group PT (μmol/L paclitaxel + 10% clean serum in the PT group, and 10% and 5% drug-containing serum and equal quantity of paclitaxel had been added into the high- and low-dosage teams, respectively.
After culturing for 24 hours, the following assessments had been carried out: (1) cell proliferation detected through the use of CCK-8 and a microplate reader; (2) axon size detected by mobile immunostaining and detection evaluation on antibody μmol/L paclitaxel + 10% clean serum in the PT group, and 10% and 5% drug-containing serum and equal quantity of paclitaxel had been added into the high- and low-dosage teams, respectively.
Experimental Study of Shenfu Injection on the Prevention and Treatment of Paclitaxel Chemotherapy DRG Neuron Injury.
After culturing for 24 hours, the following assessments had been carried out: (1) cell proliferation detected through the use of CCK-8 and a microplate reader; (2) axon size detected by mobile immunostaining and detection evaluation on antibody P < 0.05), whereas OD values of teams PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, greater than group PT (P < 0.05), whereas OD values of teams PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, greater than group PT (.Shenfu injection can forestall the toxicity of DRG neurons induced by paclitaxel, and its mechanism could also be associated to the alleviation of mitochondrial dysfunction.
DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining
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Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.
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