Experimental Study of Shenfu Injection on the Prevention and Treatment of Paclitaxel Chemotherapy DRG Neuron Injury.

The goal of this paper is investigating the impact and mechanism of Shenfu injection (a Traditional Chinese Medicine injection type) on prevention and therapy of paclitaxel chemotherapy in peripheral nerve harm.MethodsWistar rat dorsal root ganglion cells had been cultured in vitro and divided into teams of MOCK, PT, PT + LD, and PT + HD.

Each group was cultured at a complete serum focus of 10%, together with 10% clean serum in the MOCK group, 0.73 (IC30) μmol/L paclitaxel + 10% clean serum in the PT group, and 10% and 5% drug-containing serum and equal quantity of paclitaxel had been added into the high- and low-dosage teams, respectively.

After culturing for 24 hours, the following assessments had been carried out: (1) cell proliferation detected through the use of CCK-8 and a microplate reader; (2) axon size detected by mobile immunostaining and detection evaluation on antibody β-tubulin III; and (3) adjustments in mitochondrial membrane potential by analyzing immunofluorescence staining with JC-1 probe.Results(1) Cell proliferation: OD values of the MOCK group and PT group had been 0.43 ± 0.02 and 0.25 ± 0.03, respectively (P < 0.05), whereas OD values of teams PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, greater than group PT (P < 0.05), whereas OD values of teams PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, greater than group PT (μmol/L paclitaxel + 10% clean serum in the PT group, and 10% and 5% drug-containing serum and equal quantity of paclitaxel had been added into the high- and low-dosage teams, respectively.

After culturing for 24 hours, the following assessments had been carried out: (1) cell proliferation detected through the use of CCK-8 and a microplate reader; (2) axon size detected by mobile immunostaining and detection evaluation on antibody μmol/L paclitaxel + 10% clean serum in the PT group, and 10% and 5% drug-containing serum and equal quantity of paclitaxel had been added into the high- and low-dosage teams, respectively.

Experimental Study of Shenfu Injection on the Prevention and Treatment of Paclitaxel Chemotherapy DRG Neuron Injury.
Experimental Study of Shenfu Injection on the Prevention and Treatment of Paclitaxel Chemotherapy DRG Neuron Injury.

After culturing for 24 hours, the following assessments had been carried out: (1) cell proliferation detected through the use of CCK-8 and a microplate reader; (2) axon size detected by mobile immunostaining and detection evaluation on antibody P < 0.05), whereas OD values of teams PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, greater than group PT (P < 0.05), whereas OD values of teams PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, greater than group PT (.Shenfu injection can forestall the toxicity of DRG neurons induced by paclitaxel, and its mechanism could also be associated to the alleviation of mitochondrial dysfunction.

DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining

AR1177 50mL(500 assys)
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STAT Double Staining ragent for Double staining Mo

NB330DB 25 ml
EUR 595
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NB326DB 25 ml
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PI Staining Kit

abx097208-1Kit 1 Kit
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Coplin Staining Jar

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Cell Staining Buffer

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?-Galactosidase Staining Kit

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Cell Cycle Staining Kit

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Cell Cycle Staining Kit

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PI / RNase Staining Buffer

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HISTOCHEMISTRY STAINING TRAY

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Exosome fluorescent staining

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Stayright™ Purple HRP Staining Kit

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Stayright™ Purple HRP Staining Kit

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Hellandahl Staining Jar+Lid 16 slide

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Four color suit nuclear staining Kit

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Fluorescein Active Caspase Staining Kit

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Foxp3/Transcription Factor Staining Kit

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Coplin Staining Jar Glass+Lid 10Slide

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EasyDip Slide Staining Jar - Green, each

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BRAND(TM) STAINING TRAY FOR 25 SLIDES

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BRAND(TM) STAINING TRAY FOR 20 SLIDES

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Yeast Viability Staining Kit (Green/Red)

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Gel-FAST? Gel Staining/Destaining Kit

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EasyDip Slide Staining Jar - Yellow, each

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Senescence β-Galactosidase Staining Kit

KTA3030-100T 100 T
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Description: Senescence β-galactosidase staining kit provides a simple and convenient operation method for staining detection of senescent cells or tissues; This kit only stains senescent cells, and does not stain pre-senescence cells (senescent cells), quiescent cells (static cells), immortal cells (immortal cells) or tumor cells.

CaspGLOW Active Caspase 12 Staining Kit

55R-1291 25 assays
EUR 338.4
Description: Caspase 12 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 2 Staining Kit

55R-1293 25 assays
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Description: Caspase 2 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 3 Staining Kit

55R-1294 25 assays
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Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 8 Staining Kit

55R-1295 25 assays
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Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 9 Staining Kit

55R-1296 25 assays
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Description: Caspase 9 Staining Kit for use activity in the research laboratory

dsGreen Gel Staining Solution, 10000×, 0.5 mL

10010 0.5 mL
EUR 106.8

dsGreen Gel Staining Solution, 10000×, 50 uL

A0010 50 uL
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Mouse to Mouse HRP (DAB) Staining System

MTM001 70 Slides
EUR 188.4

Mouse to Mouse HRP (AEC) Staining System

MTM002 70 Slides
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Live and Dead Cell Double Staining Kit

KTA1001-100T 100 T
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Description: Abbkine Live and Dead Cell Double Staining Kit provides a convenient assay to evaluate the viability of cells.

Live and Dead Cell Double Staining Kit

KTA1001-2000T 2000 T
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Description: Abbkine Live and Dead Cell Double Staining Kit provides a convenient assay to evaluate the viability of cells.

Live and Dead Cell Double Staining Kit

KTA1001-500T 500 T
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Description: Abbkine Live and Dead Cell Double Staining Kit provides a convenient assay to evaluate the viability of cells.

CaspGLOW Red Active Caspase Staining Kit

55R-1297 25 assays
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Coomassie Blue G-250 Staining Solution

GR103035 1 L
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CaspGLOW? Red Active Caspase Staining Kit

K190-100 each
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CaspGLOW? Red Active Caspase Staining Kit

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Nuclear 7-AAD fluorescent staining kit

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Plastic Coplin Staining Jars, pack of 12

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Glass Staining Coplin Jar with Lid, each

SD-05 -
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Coomassie Blue Staining Destaining Solution

AR0140 1kit (for 10 assays to stain gels of 5 x 8.5cm)
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Coomassie Brilliant Blue Gel Staining Kit

abx090653-1Kit 1 Kit
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SensiTek HRP Anti-Mouse Staining System

AFA600 500 Slides
EUR 258

UltraTek HRP Anti-Mouse Staining System

AFJ600 500 Slides
EUR 340.8

Staining Jar 4ml Cavity 40x40mm With lid

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SensiTek HRP Anti-Rabbit Staining System

AFE600 500 Slides
EUR 258

UltraTek HRP Anti-Rabbit Staining System

AFK600 500 Slides
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Mouse to Mouse Alk-Phos Staining System

MTM005 400 Slides
EUR 433.2

Fluorescein Active Caspase-2 Staining Kit

K2048-100 100 assays
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Fluorescein Active Caspase-2 Staining Kit

K2048-25 25 assays
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Fluorescein Active Caspase-3 Staining Kit

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Fluorescein Active Caspase-3 Staining Kit

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Fluorescein Active Caspase-8 Staining Kit

K2050-100 100 assays
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Fluorescein Active Caspase-8 Staining Kit

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Fluorescein Active Caspase-9 Staining Kit

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Fluorescein Active Caspase-9 Staining Kit

K2051-25 25 assays
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Prosi Silver Staining Kit for 25 mini gel

S3200-020 1kit
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Propidium iodide staining kit (ready to use) 

EGY0651 5mL
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Propidium iodide staining kit (ready to use) 

EGY0652 10mL
EUR 125

Propidium iodide staining kit (ready to use) 

EGY0653 20mL
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Protein Stain-EZ J Phosphoprotein Staining Kit

BSP043 2x10minigel, 20prep
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Fluorescein Active Caspase-12 Staining Kit

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Fluorescein Active Caspase-12 Staining Kit

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