Experimental Study of Shenfu Injection on the Prevention and Treatment of Paclitaxel Chemotherapy DRG Neuron Injury.

The goal of this paper is investigating the impact and mechanism of Shenfu injection (a Traditional Chinese Medicine injection type) on prevention and therapy of paclitaxel chemotherapy in peripheral nerve harm.MethodsWistar rat dorsal root ganglion cells had been cultured in vitro and divided into teams of MOCK, PT, PT + LD, and PT + HD.

Each group was cultured at a complete serum focus of 10%, together with 10% clean serum in the MOCK group, 0.73 (IC30) μmol/L paclitaxel + 10% clean serum in the PT group, and 10% and 5% drug-containing serum and equal quantity of paclitaxel had been added into the high- and low-dosage teams, respectively.

After culturing for 24 hours, the following assessments had been carried out: (1) cell proliferation detected through the use of CCK-8 and a microplate reader; (2) axon size detected by mobile immunostaining and detection evaluation on antibody β-tubulin III; and (3) adjustments in mitochondrial membrane potential by analyzing immunofluorescence staining with JC-1 probe.Results(1) Cell proliferation: OD values of the MOCK group and PT group had been 0.43 ± 0.02 and 0.25 ± 0.03, respectively (P < 0.05), whereas OD values of teams PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, greater than group PT (P < 0.05), whereas OD values of teams PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, greater than group PT (μmol/L paclitaxel + 10% clean serum in the PT group, and 10% and 5% drug-containing serum and equal quantity of paclitaxel had been added into the high- and low-dosage teams, respectively.

After culturing for 24 hours, the following assessments had been carried out: (1) cell proliferation detected through the use of CCK-8 and a microplate reader; (2) axon size detected by mobile immunostaining and detection evaluation on antibody μmol/L paclitaxel + 10% clean serum in the PT group, and 10% and 5% drug-containing serum and equal quantity of paclitaxel had been added into the high- and low-dosage teams, respectively.

Experimental Study of Shenfu Injection on the Prevention and Treatment of Paclitaxel Chemotherapy DRG Neuron Injury.
Experimental Study of Shenfu Injection on the Prevention and Treatment of Paclitaxel Chemotherapy DRG Neuron Injury.

After culturing for 24 hours, the following assessments had been carried out: (1) cell proliferation detected through the use of CCK-8 and a microplate reader; (2) axon size detected by mobile immunostaining and detection evaluation on antibody P < 0.05), whereas OD values of teams PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, greater than group PT (P < 0.05), whereas OD values of teams PT + LD and PT + HD had been 0.41 ± 0.05 and 0.46 ± 0.03, respectively, greater than group PT (.Shenfu injection can forestall the toxicity of DRG neurons induced by paclitaxel, and its mechanism could also be associated to the alleviation of mitochondrial dysfunction.

DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining

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DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining

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DAPI (hydrochloride)

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DAPI (Molecular Biology Grade)

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FluorLast? with DAPI and DABCO

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?-Galactosidase Staining Kit

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DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)

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Ponceau S Staining Solution

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Fluorescein Active Caspase-12 Staining Kit

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Fluorescein Active Caspase-8 Staining Kit

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Fluorescein Active Caspase-8 Staining Kit

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Red Active Caspase-9 Staining Kit

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Basic Fuchsin, for flagella staining, certified

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Coplin Staining Jar Glass+Lid 10Slide

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Hellandahl Staining Jar+Lid 16 slide

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Stayright™ Purple HRP Staining Kit

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Stayright™ Purple HRP Staining Kit

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CaspGLOW? Fluorescein Active Caspase Staining Kit

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CaspGLOW? Red Active Caspase Staining Kit

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Gel-FAST? Gel Staining/Destaining Kit

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DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*

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DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*

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Human 4’,6-Diamidino-2-phenylindole dihydrochloride(DAPI)ELISA kit

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STAT Double Staining reagent for Double staining of Mouse and Rabbit antibodies, 450 plus slides

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STAT Double Staining Reagent for Double staining of Mouse and Goat antibodies, 450 plus slides

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DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *10 mM solution in water*

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Cellular Senescence Detection Kit (SA-?-Gal Staining)

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Cellular Senescence Detection Kit (SA-?-Gal Staining)

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Protein Stain-EZ J Phosphoprotein Staining Kit

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SensiTek HRP Anti-Mouse (DAB) Staining System

AEA080 70 Slides
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SensiTek Alk-Phos Anti-Mouse Staining System

AFB600 500 Slides
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SensiTek Alk-Phos Anti-Rabbit Staining System

AFF600 500 Slides
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SensiTek Alk-Phos Anti-Polyvalent Staining System

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UltraTek Alk-Phos Anti-Mouse Staining System

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AFP600 500 Slides
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SensiTek HRP Anti-Mouse (AEC) Staining System

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SensiTek HRP Anti-Polyvalent (DAB) Staining System

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Blue Feulgen DNA Ploidy Analysis Staining Kit

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CaspGLOW Red Active Caspase 3 Staining Kit

55R-1298 25 assays
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Description: Caspase 3 Staining Kit for use activity in the research laboratory

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55R-1299 25 assays
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55R-1300 25 assays
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Sirius Red/Fast Green Collagen Staining Kit

9046 30-50 samples
EUR 164.4
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dsGreen Gel Staining Solution, 10000×, 0.5 mL

10010 0.5 mL
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dsGreen Gel Staining Solution, 10000×, 50 uL

A0010 50 uL
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Mouse to Mouse HRP (DAB) Staining System

MTM001 70 Slides
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Mouse to Mouse Alk-Phos Staining System

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