DRG Neurons Promote Perineural Invasion of Endometrial Cancer via GluR2.

Background: Perineural invasion (PNI) is correlated with negative prognosis in multiple cancers, but its role in endometrial cancer (EC) is still largely unknown; thus, targeted treatment for nerve infiltration is lacking as well. Methods: The interaction between nerve and EC cells were investigated by in vitro neural invasion assay and transwell coculture system.

Then the nerve-related receptor gene glutamate ionotropic receptor AMPA type subunit 2 (GRIA2) was detected in EC tissues and cells using PCR array, western blotting, and immunohistochemistry. The role of GluR2 (gene name GRIA2) on EC proliferation, migration and invasion was evaluated by a GluR2 antagonist and shRNA. At the same time, the neurotransmitter effect on GluR2 (glutamate) from the cocultured conditional medium was measured using high-performance liquid chromatography (HPLC). 

Results: EC cell line Ishikawa (ISK) showed the ability to migrate along neurites in vitro and the numbers of migrated/invaded EC cells in the DRG neuron coculture group were significantly increased. The expression of GluR2 in EC tissue was found to be higher than that in para-carcinoma tissue. After GluR2 antagonist and GluR2 shRNA treatment, the proliferation, migration and invasion of ISK cells was markedly inhibited. Moreover, the ability of DRG neurons to promote the migration and invasion of ISK cells could also be attenuated by downregulation of GluR2, and the concentration of the neurotransmitter glutamate was notably increased in the coculture conditional medium compared to that in the DRG neuron or ISK cells alone. Conclusions: DRG neurons promote metastasis of EC cells via GluR2, which might be a risk factor for PNI in EC. Moreover, the perineural system may promote tumor invasion and metastasis under certain circumstances.

 DRG Neurons Promote Perineural Invasion of Endometrial Cancer via GluR2.
DRG Neurons Promote Perineural Invasion of Endometrial Cancer via GluR2.

Cell Explorerâ„¢ Live Cell Labeling Kit *Green Fluorescence with 405 nm Excitation*

22615-200Tests 200 Tests
EUR 222
Description: Our Cell Explorer™ fluorescence imaging kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.

Cell Explorerâ„¢ Live Cell Labeling Kit *Orange Fluorescence with 405 nm Excitation*

22616-200Tests 200 Tests
EUR 222
Description: Our Cell Explorer™ fluorescence imaging kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.

Cell Navigator® Lysosome Staining Kit *Green Fluorescence with 405 nm Excitation*

22651-500Tests 500 Tests
EUR 222
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.

Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Blue Fluorescence with 405 nm Excitation*

22500-200Tests 200 Tests
EUR 166
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.

Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Green Fluorescence with 405 nm Excitation*

22501-200Tests 200 Tests
EUR 166
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.

Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Orange Fluorescence with 405 nm Excitation*

22502-200Tests 200 Tests
EUR 166
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.

Cell Navigator® Mitochondrion Staining Kit *Orange Fluorescence with 405 nm Excitation*

22673-500Assays 500 Assays
EUR 222
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria, nuclei, etc.

Cell Meterâ„¢ Intracellular GSH Assay Kit *Optimized for Flow Cytometry with 405 nm excitation*

22809-100Tests 100 Tests
EUR 222
Description: There are a variety of parameters that can be used for monitoring cell apoptosis.

Emission filter 668 - 722 nm for ChemStudio's

AJ38-0339-01 each
EUR 1596.85

Emission filter 513 - 557 nm for ChemStudio's

AJ38-0340-01 each
EUR 1596.85

Emission filter 565 - 625 nm for ChemStudio's

AJ38-0344-01 each
EUR 1596.85

Emission filter 767 - 807 nm for ChemStudio's

AJ38-0362-01 each
EUR 1596.85

Emission filter 700 - 740 nm for ChemStudio's

AJ38-0369-01 each
EUR 2610.55

Cell Meterâ„¢ Fluorimetric Live Cell Cycle Assay Kit *Optimized for 405 nm Violet Laser Excitation*

22845-100Tests 100 Tests
EUR 222
Description: The cell cycle has four sequential phases: G0/G1, S, G2, and M.

Emission filter 780 nm long pass for ChemStudio's

AJ38-0365-01 each
EUR 2528.8

Emission filter 800 nm long pass for ChemStudio's

AJ38-0368-01 each
EUR 2637.8

Emission filter 520 - 620 nm (SYBR Gold) for ChemStudio's

AJ38-0221-01 each
EUR 604.95

Emission filter 510 - 560 nm (SYBR Green) for ChemStudio's

AJ38-0219-01 each
EUR 604.95

Tide Fluorâ„¢ 8WS acid [TF8WS acid] *Near Infrared Emission*

2335-10mg 10 mg
EUR 222
Description: Tide Fluorâ„¢ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.

Tide Fluorâ„¢ 8WS amine [TF8WS amine] *Near Infrared Emission*

2336 1 mg
EUR 227
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.

Tide Fluorâ„¢ 8WS amine [TF8WS amine] *Near Infrared Emission*

2336-1mg 1 mg
EUR 222
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.

Tide Fluorâ„¢ 8WS azide [TF8WS azide] *Near Infrared Emission*

2306 1 mg
EUR 227
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.

Tide Fluorâ„¢ 8WS azide [TF8WS azide] *Near Infrared Emission*

2306-1mg 1 mg
EUR 222
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.

Tide Fluorâ„¢ 8WS alkyne [TF8WS alkyne] *Near Infrared Emission*

2307 1 mg
EUR 227
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.

Tide Fluorâ„¢ 8WS alkyne [TF8WS alkyne] *Near Infrared Emission*

2307-1mg 1 mg
EUR 222
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.

DiagNano™ Amine Quantum Dot Beads, 160 nm, 610 nm Emission Peak

DNQ-N013 1 ml
EUR 1080

DiagNano™ Streptavidin Quantum Dot Beads, 655 nm Emission Peak

DNQ-N007 1 ml
EUR 1080

DiagNano™ Streptavidin Quantum Dot Beads, 625 nm Emission Peak

DNQ-N008 1 ml
EUR 1080

DiagNano™ Streptavidin Quantum Dot Beads, 610 nm Emission Peak

DNQ-N009 1 ml
EUR 1080

DiagNano™ Streptavidin Quantum Dot Beads, 585 nm Emission Peak

DNQ-N010 1 ml
EUR 1080

DiagNano™ Streptavidin Quantum Dot Beads, 565 nm Emission Peak

DNQ-N011 1 ml
EUR 1080

DiagNano™ Streptavidin Quantum Dot Beads, 525 nm Emission Peak

DNQ-N012 1 ml
EUR 1080

DiagNano™ PEG Magnetic Quantum Dot Beads, 655 nm Emission Peak

DNQ-CQ012 150 ul
EUR 960

DiagNano™ CD4 Magnetic Quantum Dot Beads, 655 nm Emission Peak

DNQ-CQ013 150 ul
EUR 960

DiagNano™ PEG Magnetic Quantum Dot Beads, 610 nm Emission Peak

DNQ-CQ015 150 ul
EUR 960

DiagNano™ CD3 Magnetic Quantum Dot Beads, 655 nm Emission Peak

DNQ-CQ001 20 tests
EUR 1160

DiagNano™ CD4 Magnetic Quantum Dot Beads, 610 nm Emission Peak

DNQ-CQ002 20 tests
EUR 1160

DiagNano™ CD3 Magnetic Quantum Dot Beads, 610 nm Emission Peak

DNQ-CQ004 20 tests
EUR 1160

DiagNano™ CD3 Magnetic Quantum Dot Beads, 640 nm Emission Peak

DNQ-CQ006 20 tests
EUR 1160

DiagNano™ CD4 Magnetic Quantum Dot Beads, 575 nm Emission Peak

DNQ-CQ007 20 tests
EUR 1160

DiagNano™ CD4 Magnetic Quantum Dot Beads, 640 nm Emission Peak

DNQ-CQ008 20 tests
EUR 1160

DiagNano™ PEG Magnetic Quantum Dot Beads, 640 nm Emission Peak

DNQ-CQ009 150 ul
EUR 960

DiagNano™ PEG Magnetic Quantum Dot Beads, 575 nm Emission Peak

DNQ-CQ010 150 ul
EUR 960

DiagNano™ CD3 Magnetic Quantum Dot Beads, 575 nm Emission Peak

DNQ-CQ011 20 tests
EUR 1160

DiagNano™ CD45 Magnetic Quantum Dot Beads, 655 nm Emission Peak

DNQ-CQ014 20 tests
EUR 1160

DiagNano™ CD45 Magnetic Quantum Dot Beads, 610 nm Emission Peak

DNQ-CQ016 20 tests
EUR 1160

DiagNano™ CD45 Magnetic Quantum Dot Beads, 640 nm Emission Peak

DNQ-CQ003 20 tests
EUR 1160

DiagNano™ CD45 Magnetic Quantum Dot Beads, 575 nm Emission Peak

DNQ-CQ005 20 tests
EUR 1160

DiagNano™ Carboxyl Quantum Dot Beads, 120 nm, 610 nm Emission Peak

DNQ-N001 1 ml
EUR 1080

DiagNano™ Carboxyl Quantum Dot Beads, 120 nm, 625 nm Emission Peak

DNQ-N002 1 ml
EUR 1080

DiagNano™ Carboxyl Quantum Dot Beads, 140 nm, 655 nm Emission Peak

DNQ-N003 1 ml
EUR 1080

DiagNano™ Carboxyl Quantum Dot Beads, 120 nm, 585 nm Emission Peak

DNQ-N004 1 ml
EUR 1080

DiagNano™ Carboxyl Quantum Dot Beads, 140 nm, 565 nm Emission Peak

DNQ-N005 1 ml
EUR 1080

DiagNano™ Carboxyl Quantum Dot Beads, 160 nm, 525 nm Emission Peak

DNQ-N006 1 ml
EUR 1080

Tide Fluorâ„¢ 8WS maleimide [TF8WS maleimide] *Near Infrared Emission*

2337 1 mg
EUR 227
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.

Tide Fluorâ„¢ 8WS maleimide [TF8WS maleimide] *Near Infrared Emission*

2337-1mg 1 mg
EUR 222
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.

Tide Fluorâ„¢ 8WS, succinimidyl ester [TF8WS SE]*Near Infrared Emission*

2338 1 mg
EUR 112
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.

Tide Fluorâ„¢ 8WS, succinimidyl ester [TF8WS SE]*Near Infrared Emission*

2338-1mg 1 mg
EUR 109
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.

DAPI

abx082063-10mg 10 mg
EUR 276

DAPI

40011-1 EA
EUR 122

DAPI

BMD0063-100mg 100 mg
EUR 849
Description: DAPI is a popular blue fluorescent DNA dye.

DAPI

BMD0063-10mg 10 mg
EUR 119
Description: DAPI is a popular blue fluorescent DNA dye.

DAPI

B1098-25 each
EUR 496.8

DAPI

B1098-5 each
EUR 170.4

DAPI

abx283157-100g 100 µg Ask for price

DAPI

abx283157-20g 20 µg
EUR 106.25

DAPI

abx283157-50g 50 µg
EUR 237.5

DAPI

abx082063-100l 100 µl
EUR 212.5

DAPI

abx082063-1ml 1 ml Ask for price

DAPI

abx082063-200l 200 µl Ask for price

DAPI

BMD0063-each each Ask for price

DAPI

MBS9719177-100mg 100mg
EUR 605

DAPI

MBS9719177-10mg 10mg
EUR 155

DAPI

MBS9719177-5x100mg 5x100mg
EUR 2685

DAPI Reagent

MBS2567122-100Tests 100Tests
EUR 85

DAPI Reagent

MBS2567122-200Tests 200Tests
EUR 95

DAPI Reagent

MBS2567122-500Tests 500Tests
EUR 115

DAPI Reagent

MBS2567122-50Tests 50Tests
EUR 80

DAPI Reagent

MBS2567122-5x500Tests 5x500Tests
EUR 510

DAPI Reagent

MBS2563514-10mL 10mL
EUR 100

DAPI Reagent

MBS2563514-50mL 50mL
EUR 150

DAPI Reagent

MBS2563514-5mL 5mL
EUR 90

DAPI Reagent

MBS2563514-5x50mL 5x50mL
EUR 640

DAPI Dilactate

17509 25 mg
EUR 112
Description: DAPI is a fluorescent stain that binds strongly to DNA.

DAPI Dilactate

17509-25mg 25 mg
EUR 109
Description: DAPI is a fluorescent stain that binds strongly to DNA.

DAPI (hydrochloride)

C3362-10 10 mg
EUR 62
Description: A fluorescent probe, commonly used to stain DNA and chromosomes

DAPI (hydrochloride)

C3362-25 25 mg
EUR 130
Description: A fluorescent probe, commonly used to stain DNA and chromosomes

DAPI (hydrochloride)

C3362-5 5 mg
EUR 37
Description: A fluorescent probe, commonly used to stain DNA and chromosomes

DAPI (Hydrochloride)

MBS696332-25mg 25mg
EUR 260

DAPI (Hydrochloride)

MBS696332-5mg 5mg
EUR 150

DAPI (Hydrochloride)

MBS696332-5x25mg 5x25mg
EUR 1120

DAPI Dihydrochloride

561706 5.0mg
EUR 190

DAPI dihydrochloride

MB097-10MG 1 unit
EUR 41.98
Description: DAPI dihydrochloride

DAPI dihydrochloride

MB097-25MG 1 unit
EUR 83.31
Description: DAPI dihydrochloride

DAPI dihydrochloride

RM5773-25MG 1 unit
EUR 119.85
Description: DAPI dihydrochloride

DAPI (dihydrochloride)

HY-D0814 50mg
EUR 439.2

DAPI Dihydrochloride

T19827-10mg 10mg Ask for price
Description: DAPI Dihydrochloride

DAPI Dihydrochloride

T19827-1g 1g Ask for price
Description: DAPI Dihydrochloride

DAPI Dihydrochloride

T19827-1mg 1mg Ask for price
Description: DAPI Dihydrochloride

DAPI Dihydrochloride

T19827-50mg 50mg Ask for price
Description: DAPI Dihydrochloride

DAPI Dihydrochloride

T19827-5mg 5mg Ask for price
Description: DAPI Dihydrochloride

DAPI Dihydrochloride

MBS5756233-100mg 100mg
EUR 390

DAPI Dihydrochloride

MBS5756233-10mg 10mg
EUR 165

DAPI Dihydrochloride

MBS5756233-25mg 25mg
EUR 225

DAPI Dihydrochloride

MBS5756233-50mg 50mg
EUR 315

DAPI Dihydrochloride

MBS5756233-5mg 5mg
EUR 155

Excitatory amino acid transporter 1

AP79277 1mg
EUR 2640

Excitatory amino acid transporter 1

AP82681 1mg
EUR 2640

Excitatory amino acid transporter 1

AP82767 1mg
EUR 2640

Excitatory amino acid transporter 1

AP82881 1mg
EUR 2640

Rat Excitatory amino acid transporter 1 ELISA Kit

EK4236 96 tests
EUR 599

Rat Excitatory Amino Acid Transporter 4 ELISA Kit

MBS096623-10x96StripWells 10x96-Strip-Wells
EUR 6725

Rat Excitatory Amino Acid Transporter 4 ELISA Kit

MBS096623-48StripWells 48-Strip-Wells
EUR 550

Rat Excitatory Amino Acid Transporter 4 ELISA Kit

MBS096623-5x96StripWells 5x96-Strip-Wells
EUR 3420

Rat Excitatory Amino Acid Transporter 4 ELISA Kit

MBS096623-96StripWells 96-Strip-Wells
EUR 765

Cat Excitatory Amino Acid Transporter 5 ELISA Kit

MBS097604-INQUIRE INQUIRE Ask for price

Rat Excitatory Amino Acid Transporter 5 ELISA Kit

MBS102078-10x96StripWells 10x96-Strip-Wells
EUR 6725

Rat Excitatory Amino Acid Transporter 5 ELISA Kit

MBS102078-48StripWells 48-Strip-Wells
EUR 550

Rat Excitatory Amino Acid Transporter 5 ELISA Kit

MBS102078-5x96StripWells 5x96-Strip-Wells
EUR 3420

Rat Excitatory Amino Acid Transporter 5 ELISA Kit

MBS102078-96StripWells 96-Strip-Wells
EUR 765

Rat Excitatory Amino Acid Transporter 2 ELISA Kit

MBS097722-10x96StripWells 10x96-Strip-Wells
EUR 6725

Rat Excitatory Amino Acid Transporter 2 ELISA Kit

MBS097722-48StripWells 48-Strip-Wells
EUR 550

Rat Excitatory Amino Acid Transporter 2 ELISA Kit

MBS097722-5x96StripWells 5x96-Strip-Wells
EUR 3420

Rat Excitatory Amino Acid Transporter 2 ELISA Kit

MBS097722-96StripWells 96-Strip-Wells
EUR 765

Cat Excitatory Amino Acid Transporter 3 ELISA Kit

MBS049528-INQUIRE INQUIRE Ask for price

Rat Excitatory Amino Acid Transporter 1 ELISA Kit

MBS050004-10x96StripWells 10x96-Strip-Wells
EUR 6725

Rat Excitatory Amino Acid Transporter 1 ELISA Kit

MBS050004-48StripWells 48-Strip-Wells
EUR 550

Rat Excitatory Amino Acid Transporter 1 ELISA Kit

MBS050004-5x96StripWells 5x96-Strip-Wells
EUR 3420

Rat Excitatory Amino Acid Transporter 1 ELISA Kit

MBS050004-96StripWells 96-Strip-Wells
EUR 765

Rat Excitatory Amino Acid Transporter 3 ELISA Kit

MBS074839-10x96StripWells 10x96-Strip-Wells
EUR 6725

Rat Excitatory Amino Acid Transporter 3 ELISA Kit

MBS074839-48StripWells 48-Strip-Wells
EUR 550

Rat Excitatory Amino Acid Transporter 3 ELISA Kit

MBS074839-5x96StripWells 5x96-Strip-Wells
EUR 3420

Rat Excitatory Amino Acid Transporter 3 ELISA Kit

MBS074839-96StripWells 96-Strip-Wells
EUR 765

Cat Excitatory Amino Acid Transporter 1 ELISA Kit

MBS075267-INQUIRE INQUIRE Ask for price

Cat Excitatory Amino Acid Transporter 4 ELISA Kit

MBS065343-INQUIRE INQUIRE Ask for price

Cat Excitatory Amino Acid Transporter 2 ELISA Kit

MBS079198-INQUIRE INQUIRE Ask for price

Rat Excitatory Amino Acid Transporter 2 ELISA Kit

IRTEAAT2KT each
EUR 790
Description: Rat Excitatory Amino Acid Transporter 2 ELISA Kit

Rat Excitatory amino acid transporter 1 ELISA Kit

MBS762950-10x96StripWells 10x96-Strip-Wells
EUR 3900

Rat Excitatory amino acid transporter 1 ELISA Kit

MBS762950-48StripWells 48-Strip-Wells
EUR 340

Rat Excitatory amino acid transporter 1 ELISA Kit

MBS762950-5x96StripWells 5x96-Strip-Wells
EUR 2045

Rat Excitatory amino acid transporter 1 ELISA Kit

MBS762950-96StripWells 96-Strip-Wells
EUR 455

Rat Excitatory amino acid transporter 1 ELISA Kit

MBS2882261-10x96StripWells 10x96-Strip-Wells
EUR 4080

Rat Excitatory amino acid transporter 1 ELISA Kit

MBS2882261-48StripWells 48-Strip-Wells
EUR 390

Rat Excitatory amino acid transporter 1 ELISA Kit

MBS2882261-5x96StripWells 5x96-Strip-Wells
EUR 2220

Rat Excitatory amino acid transporter 1 ELISA Kit

MBS2882261-96StripWells 96-Strip-Wells
EUR 520

Rat Excitatory amino acid transporter 1 ELISA Kit

MBS9428062-5x96Tests 5x96Tests
EUR 2820

Rat Excitatory amino acid transporter 1 ELISA Kit

MBS9428062-96Tests 96Tests
EUR 615

DAPI Staining Kit

abx097206-1Kit 1 Kit
EUR 276

DAPI Staining Kit

abx097206-100l 100 µl
EUR 162.5

DAPI Staining Kit

abx097206-1ml 1 ml Ask for price

DAPI Staining Kit

abx097206-200l 200 µl Ask for price

DAPI Staining Kit

MBS826728-01mL 0.1mL
EUR 140

DAPI Staining Kit

MBS826728-5x01mL 5x0.1mL
EUR 550

Duck Excitatory Amino Acid Transporter 5 ELISA Kit

MBS092404-INQUIRE INQUIRE Ask for price

Goat Excitatory Amino Acid Transporter 5 ELISA Kit

MBS098107-INQUIRE INQUIRE Ask for price

Duck Excitatory Amino Acid Transporter 2 ELISA Kit

MBS098292-INQUIRE INQUIRE Ask for price

Goat Excitatory Amino Acid Transporter 1 ELISA Kit

MBS052283-INQUIRE INQUIRE Ask for price

Goat Excitatory Amino Acid Transporter 4 ELISA Kit

MBS057124-INQUIRE INQUIRE Ask for price

Fish Excitatory Amino Acid Transporter 4 ELISA Kit

MBS073948-INQUIRE INQUIRE Ask for price

Cavy Excitatory Amino Acid Transporter 2 ELISA Kit

MBS075064-INQUIRE INQUIRE Ask for price

Duck Excitatory Amino Acid Transporter 3 ELISA Kit

MBS071975-INQUIRE INQUIRE Ask for price

Goat Excitatory Amino Acid Transporter 3 ELISA Kit

MBS064052-INQUIRE INQUIRE Ask for price

Duck Excitatory Amino Acid Transporter 1 ELISA Kit

MBS064469-INQUIRE INQUIRE Ask for price

Fish Excitatory Amino Acid Transporter 5 ELISA Kit

MBS064930-INQUIRE INQUIRE Ask for price

Fish Excitatory Amino Acid Transporter 3 ELISA Kit

MBS067173-INQUIRE INQUIRE Ask for price

Cavy Excitatory Amino Acid Transporter 3 ELISA Kit

MBS078706-INQUIRE INQUIRE Ask for price

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