Background: Perineural invasion (PNI) is correlated with negative prognosis in multiple cancers, but its role in endometrial cancer (EC) is still largely unknown; thus, targeted treatment for nerve infiltration is lacking as well. Methods: The interaction between nerve and EC cells were investigated by in vitro neural invasion assay and transwell coculture system.
Then the nerve-related receptor gene glutamate ionotropic receptor AMPA type subunit 2 (GRIA2) was detected in EC tissues and cells using PCR array, western blotting, and immunohistochemistry. The role of GluR2 (gene name GRIA2) on EC proliferation, migration and invasion was evaluated by a GluR2 antagonist and shRNA. At the same time, the neurotransmitter effect on GluR2 (glutamate) from the cocultured conditional medium was measured using high-performance liquid chromatography (HPLC).
Results: EC cell line Ishikawa (ISK) showed the ability to migrate along neurites in vitro and the numbers of migrated/invaded EC cells in the DRG neuron coculture group were significantly increased. The expression of GluR2 in EC tissue was found to be higher than that in para-carcinoma tissue. After GluR2 antagonist and GluR2 shRNA treatment, the proliferation, migration and invasion of ISK cells was markedly inhibited. Moreover, the ability of DRG neurons to promote the migration and invasion of ISK cells could also be attenuated by downregulation of GluR2, and the concentration of the neurotransmitter glutamate was notably increased in the coculture conditional medium compared to that in the DRG neuron or ISK cells alone. Conclusions: DRG neurons promote metastasis of EC cells via GluR2, which might be a risk factor for PNI in EC. Moreover, the perineural system may promote tumor invasion and metastasis under certain circumstances.
DRG Neurons Promote Perineural Invasion of Endometrial Cancer via GluR2.
Cell Explorerâ„¢ Live Cell Labeling Kit *Green Fluorescence with 405 nm Excitation*
Description: Our Cell Explorer™ fluorescence imaging kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.
Cell Explorer™ Live Cell Labeling Kit *Blue Fluorescence with 405 nm Excitation*
Description: Our Cell Explorer™ fluorescence imaging kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.
Cell Explorer™ Live Cell Labeling Kit *Green Fluorescence with 405 nm Excitation*
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.
Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Blue Fluorescence with 405 nm Excitation*
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.
Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Orange Fluorescence with 405 nm Excitation*
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria, nuclei, etc.
Cell Meterâ„¢ Intracellular GSH Assay Kit *Optimized for Flow Cytometry with 405 nm excitation*
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Excitatory amino acid transporter 1 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Excitatory Amino Acid Transporter 2 (EAAT2) in samples from tissue homogenates, cell lysates or other biological fluids.
Rat Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Excitatory Amino Acid Transporter 2 (EAAT2) in samples from tissue homogenates, cell lysates or other biological fluids.
Rat Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Excitatory Amino Acid Transporter 2 (EAAT2) in Tissue homogenates, cell lysates and other biological fluids.
Rat Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Excitatory Amino Acid Transporter 2 (EAAT2) in Tissue homogenates, cell lysates and other biological fluids.
Rat Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Excitatory Amino Acid Transporter 2 (EAAT2) in Tissue homogenates, cell lysates and other biological fluids.
Rat Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Excitatory Amino Acid Transporter 2 (EAAT2) in Tissue homogenates, cell lysates and other biological fluids.
Rat Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Excitatory Amino Acid Transporter 2 (EAAT2) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Rat Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit
Description: A sandwich ELISA kit for quantitative measurement of Rat EAAT3 (Excitatory Amino Acid Transporter 3) in samples from Serum, Plasma, Cell supernatant
Rat Slc1a3(Excitatory amino acid transporter 1) ELISA Kit
Description: A sandwich ELISA kit for detection of Excitatory Amino Acid Transporter 2 from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Human Excitatory Amino Acid Transporter 1 (EAAT1) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Excitatory Amino Acid Transporter 1 (EAAT1) in samples from tissue homogenates or other biological fluids.
Human Excitatory Amino Acid Transporter 1 (EAAT1) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Excitatory Amino Acid Transporter 1 (EAAT1) in samples from tissue homogenates or other biological fluids.
Human Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Excitatory Amino Acid Transporter 2 (EAAT2) in samples from tissue homogenates, cell lysates or other biological fluids.
Human Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Excitatory Amino Acid Transporter 2 (EAAT2) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Excitatory Amino Acid Transporter 2 (EAAT2) in samples from tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Excitatory Amino Acid Transporter 2 (EAAT2) in samples from tissue homogenates or other biological fluids.
Human Excitatory Amino Acid Transporter 3 (EAAT3) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Excitatory Amino Acid Transporter 3 (EAAT3) in samples from tissue homogenates or other biological fluids.
Human Excitatory Amino Acid Transporter 3 (EAAT3) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Excitatory Amino Acid Transporter 3 (EAAT3) in samples from tissue homogenates or other biological fluids.
Human Excitatory Amino Acid Transporter 4 (EAAT4) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Excitatory Amino Acid Transporter 4 (EAAT4) in samples from tissue homogenates or other biological fluids.
Human Excitatory Amino Acid Transporter 4 (EAAT4) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Excitatory Amino Acid Transporter 4 (EAAT4) in samples from tissue homogenates or other biological fluids.
Human Excitatory Amino Acid Transporter 5 (EAAT5) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Excitatory Amino Acid Transporter 5 (EAAT5) in samples from tissue homogenates or other biological fluids.
Human Excitatory Amino Acid Transporter 5 (EAAT5) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Excitatory Amino Acid Transporter 5 (EAAT5) in samples from tissue homogenates or other biological fluids.
ELISA kit for Rat Excitatory amino acid transporter 2 (SLC1A2)
Description: Quantitative sandwich ELISA for measuring Rat Excitatory amino acid transporter 2 (SLC1A2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Rat Excitatory amino acid transporter 2 (SLC1A2)
Description: Quantitative sandwich ELISA for measuring Rat Excitatory amino acid transporter 2 (SLC1A2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Rat Excitatory amino acid transporter 2 (SLC1A2)
Description: Quantitative sandwich ELISA for measuring Rat Excitatory amino acid transporter 2 (SLC1A2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Human Excitatory Amino Acid Transporter 5 (EAAT5) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 5 (EAAT5) in Tissue homogenates and other biological fluids.
Human Excitatory Amino Acid Transporter 5 (EAAT5) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 5 (EAAT5) in Tissue homogenates and other biological fluids.
Human Excitatory Amino Acid Transporter 5 (EAAT5) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 5 (EAAT5) in Tissue homogenates and other biological fluids.
Human Excitatory Amino Acid Transporter 5 (EAAT5) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 5 (EAAT5) in Tissue homogenates and other biological fluids.
Human Excitatory Amino Acid Transporter 5 (EAAT5) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Excitatory Amino Acid Transporter 5 (EAAT5) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Human Excitatory Amino Acid Transporter 4 (EAAT4) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 4 (EAAT4) in Tissue homogenates and other biological fluids.
Human Excitatory Amino Acid Transporter 4 (EAAT4) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 4 (EAAT4) in Tissue homogenates and other biological fluids.
Human Excitatory Amino Acid Transporter 4 (EAAT4) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 4 (EAAT4) in Tissue homogenates and other biological fluids.
Human Excitatory Amino Acid Transporter 4 (EAAT4) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 4 (EAAT4) in Tissue homogenates and other biological fluids.
Human Excitatory Amino Acid Transporter 4 (EAAT4) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Excitatory Amino Acid Transporter 4 (EAAT4) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Human Excitatory Amino Acid Transporter 1 (EAAT1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 3 (EAAT3) in Tissue homogenates and other biological fluids.
Human Excitatory Amino Acid Transporter 3 (EAAT3) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 3 (EAAT3) in Tissue homogenates and other biological fluids.
Human Excitatory Amino Acid Transporter 3 (EAAT3) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 3 (EAAT3) in Tissue homogenates and other biological fluids.
Human Excitatory Amino Acid Transporter 3 (EAAT3) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 3 (EAAT3) in Tissue homogenates and other biological fluids.
Human Excitatory Amino Acid Transporter 3 (EAAT3) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Excitatory Amino Acid Transporter 3 (EAAT3) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Human Excitatory Amino Acid Transporter 1 (EAAT1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 1 (EAAT1) in Tissue homogenates and other biological fluids.
Human Excitatory Amino Acid Transporter 1 (EAAT1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 1 (EAAT1) in Tissue homogenates and other biological fluids.
Human Excitatory Amino Acid Transporter 1 (EAAT1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 1 (EAAT1) in Tissue homogenates and other biological fluids.
Human Excitatory Amino Acid Transporter 1 (EAAT1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 1 (EAAT1) in Tissue homogenates and other biological fluids.
Human Excitatory Amino Acid Transporter 1 (EAAT1) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Excitatory Amino Acid Transporter 1 (EAAT1) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Excitatory Amino Acid Transporter 1 (EAAT1) in Tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Excitatory Amino Acid Transporter 1 (EAAT1) in Tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Excitatory Amino Acid Transporter 1 (EAAT1) in Tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Excitatory Amino Acid Transporter 1 (EAAT1) in Tissue homogenates, cell lysates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Excitatory Amino Acid Transporter 1 (EAAT1) in samples from Tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Human Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 2 (EAAT2) in Tissue homogenates, cell lysates and other biological fluids.
Human Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 2 (EAAT2) in Tissue homogenates, cell lysates and other biological fluids.
Human Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 2 (EAAT2) in Tissue homogenates, cell lysates and other biological fluids.
Human Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Excitatory Amino Acid Transporter 2 (EAAT2) in Tissue homogenates, cell lysates and other biological fluids.
Human Excitatory Amino Acid Transporter 2 (EAAT2) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Excitatory Amino Acid Transporter 2 (EAAT2) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Excitatory Amino Acid Transporter 2 (EAAT2) in tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Excitatory Amino Acid Transporter 2 (EAAT2) in tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Excitatory Amino Acid Transporter 2 (EAAT2) in tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Excitatory Amino Acid Transporter 2 (EAAT2) in tissue homogenates, cell lysates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Excitatory Amino Acid Transporter 2 (EAAT2) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Human Excitatory Amino Acid Transporter 1 (EAAT1) ELISA Kit