Dorsal root ganglion (DRG) neurons course of ache signaling by specialised nociceptors situated in their peripheral endings. It has lengthy been established low voltage-activated (LVA) CaV 3.2 calcium channels management neuronal excitability throughout sensory notion in these neurons. Silencing CaV 3.2 exercise with antisense RNA or genetic ablation outcomes in anti-nociceptive, anti-hyperalgesic and anti-allodynic results. CaV 3.2 channels are regulated by many proteins (Weiss and Zamponi, 2017), together with KLHL1, a neuronal actin-binding protein that stabilize s channel exercise by recycling it again to the plasma membrane by the recycling endosome.
We explored whether or not manipulation of KLHL1 ranges and thereby operate as a CaV 3.2 modifier can modulate DRG excitability and mechanical ache transmission or sensitivity to ache. We first assessed the mechanical sensitivity threshold and DRG properties in the KLHL1 KO mouse mannequin. KO DRG neurons exhibited smaller T-type present density in contrast to WT with out important modifications in voltage dependence, as anticipated in the absence of its modulator.
Western blot evaluation confirmed CaV 3.2 however not CaV 3.1, CaV 3.3, CaV 2.1, or CaV 2.2 protein ranges have been considerably decreased; and lowered neuron excitability and decreased ache sensitivity have been additionally discovered in the KLHL1 KO mannequin. Analogously, transient down-regulation of KLHL1 ranges in WT mice with viral supply of anti-KLHL1 shRNA additionally resulted in decreased ache sensitivity. These two experimental approaches affirm KLHL1 as a physiological modulator of excitability and ache sensitivity, offering a novel goal to management peripheral ache.
Electroacupuncture (EA) has efficient analgesic results. Our earlier research demonstrated that the upregulation of P2X3 receptors in the dorsal root ganglia (DRG) may take part in heroin withdrawal-induced hyperalgesia. The goal of this research is to additional discover whether or not 2 Hz EA reduces heroin relapse related to its analgesic impact and whether or not P2X3 receptors in the DRG are concerned in this course of. 2 Hz EA was adopted to deal with the heroin SA rats in the current research. Heroin-seeking and ache sensitivity have been evaluated.
The expression of P2X3 receptors in the DRG was detected. Our outcomes confirmed that in contrast with the management group, the reinstatement, thermal hyperalgesia, and mechanical allodynia of the heroin-addicted group have been elevated considerably. The expression of P2X3 receptors in the DRG was elevated markedly. After being handled utilizing 2 Hz EA, reinstatement was lowered, hyperalgesia was decreased, and the upregulated expression of P2X3 receptors in the DRG had decreased considerably in contrast to that in the heroin-addicted group.
KLHL1 Controls CaV3.2 Expression in DRG Neurons and Mechanical Sensitivity to Pain.
Consequently, our outcomes indicated that 2 Hz EA was an efficient technique for treating heroin-induced hyperalgesia and serving to stop relapse, and the potential mechanism may be associated to the downregulation of P2X3 receptor expression in the DRG.
Emission filter 700 - 740 nm for ChemStudio's
AJ38-0369-01
Westburg
each
EUR 2610.55
Emission filter 780 nm long pass for ChemStudio's
AJ38-0365-01
Westburg
each
EUR 2528.8
Emission filter 800 nm long pass for ChemStudio's
AJ38-0368-01
Westburg
each
EUR 2637.8
Emission filter 520 - 620 nm (SYBR Gold) for ChemStudio's
AJ38-0221-01
Westburg
each
EUR 604.95
Emission filter 510 - 560 nm (SYBR Green) for ChemStudio's
AJ38-0219-01
Westburg
each
EUR 604.95
Tide Fluorâ„¢ 8WS acid [TF8WS acid] *Near Infrared Emission*
2335-10mg
AAT Bioquest
10 mg
EUR 222
Description: Tide Fluorâ„¢ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.
Tide Fluorâ„¢ 8WS azide [TF8WS azide] *Near Infrared Emission*
2306
AAT Bioquest
1 mg
EUR 227
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.
Tide Fluorâ„¢ 8WS azide [TF8WS azide] *Near Infrared Emission*
2306-1mg
AAT Bioquest
1 mg
EUR 222
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.
Tide Fluorâ„¢ 8WS amine [TF8WS amine] *Near Infrared Emission*
2336
AAT Bioquest
1 mg
EUR 227
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.
Tide Fluorâ„¢ 8WS amine [TF8WS amine] *Near Infrared Emission*
2336-1mg
AAT Bioquest
1 mg
EUR 222
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.
Tide Fluorâ„¢ 8WS alkyne [TF8WS alkyne] *Near Infrared Emission*
2307
AAT Bioquest
1 mg
EUR 227
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.
Tide Fluorâ„¢ 8WS alkyne [TF8WS alkyne] *Near Infrared Emission*
2307-1mg
AAT Bioquest
1 mg
EUR 222
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.
DiagNano™ Amine Quantum Dot Beads, 160 nm, 610 nm Emission Peak
DNQ-N013
Creative Diagnostics
1 ml
EUR 864
DiagNano™ Streptavidin Quantum Dot Beads, 655 nm Emission Peak
DNQ-N007
Creative Diagnostics
1 ml
EUR 864
DiagNano™ Streptavidin Quantum Dot Beads, 625 nm Emission Peak
DNQ-N008
Creative Diagnostics
1 ml
EUR 864
DiagNano™ Streptavidin Quantum Dot Beads, 610 nm Emission Peak
DNQ-N009
Creative Diagnostics
1 ml
EUR 864
DiagNano™ Streptavidin Quantum Dot Beads, 585 nm Emission Peak
DNQ-N010
Creative Diagnostics
1 ml
EUR 864
DiagNano™ Streptavidin Quantum Dot Beads, 565 nm Emission Peak
DNQ-N011
Creative Diagnostics
1 ml
EUR 864
DiagNano™ Streptavidin Quantum Dot Beads, 525 nm Emission Peak
DNQ-N012
Creative Diagnostics
1 ml
EUR 864
DiagNano™ CD3 Magnetic Quantum Dot Beads, 655 nm Emission Peak
DNQ-CQ001
Creative Diagnostics
20 tests
EUR 928
DiagNano™ CD4 Magnetic Quantum Dot Beads, 610 nm Emission Peak
DNQ-CQ002
Creative Diagnostics
20 tests
EUR 928
DiagNano™ CD3 Magnetic Quantum Dot Beads, 610 nm Emission Peak
DNQ-CQ004
Creative Diagnostics
20 tests
EUR 928
DiagNano™ CD3 Magnetic Quantum Dot Beads, 640 nm Emission Peak
DNQ-CQ006
Creative Diagnostics
20 tests
EUR 928
DiagNano™ CD4 Magnetic Quantum Dot Beads, 575 nm Emission Peak
DNQ-CQ007
Creative Diagnostics
20 tests
EUR 928
DiagNano™ CD4 Magnetic Quantum Dot Beads, 640 nm Emission Peak
DNQ-CQ008
Creative Diagnostics
20 tests
EUR 928
DiagNano™ PEG Magnetic Quantum Dot Beads, 640 nm Emission Peak
DNQ-CQ009
Creative Diagnostics
150 ul
EUR 768
DiagNano™ PEG Magnetic Quantum Dot Beads, 575 nm Emission Peak
DNQ-CQ010
Creative Diagnostics
150 ul
EUR 768
DiagNano™ CD3 Magnetic Quantum Dot Beads, 575 nm Emission Peak
DNQ-CQ011
Creative Diagnostics
20 tests
EUR 928
DiagNano™ PEG Magnetic Quantum Dot Beads, 655 nm Emission Peak
DNQ-CQ012
Creative Diagnostics
150 ul
EUR 768
DiagNano™ CD4 Magnetic Quantum Dot Beads, 655 nm Emission Peak
DNQ-CQ013
Creative Diagnostics
150 ul
EUR 768
DiagNano™ PEG Magnetic Quantum Dot Beads, 610 nm Emission Peak
DNQ-CQ015
Creative Diagnostics
150 ul
EUR 768
DiagNano™ CD45 Magnetic Quantum Dot Beads, 640 nm Emission Peak
DNQ-CQ003
Creative Diagnostics
20 tests
EUR 928
DiagNano™ CD45 Magnetic Quantum Dot Beads, 575 nm Emission Peak
DNQ-CQ005
Creative Diagnostics
20 tests
EUR 928
DiagNano™ CD45 Magnetic Quantum Dot Beads, 655 nm Emission Peak
DNQ-CQ014
Creative Diagnostics
20 tests
EUR 928
DiagNano™ CD45 Magnetic Quantum Dot Beads, 610 nm Emission Peak
DNQ-CQ016
Creative Diagnostics
20 tests
EUR 928
DiagNano™ Carboxyl Quantum Dot Beads, 120 nm, 610 nm Emission Peak
DNQ-N001
Creative Diagnostics
1 ml
EUR 864
DiagNano™ Carboxyl Quantum Dot Beads, 120 nm, 625 nm Emission Peak
DNQ-N002
Creative Diagnostics
1 ml
EUR 864
DiagNano™ Carboxyl Quantum Dot Beads, 140 nm, 655 nm Emission Peak
DNQ-N003
Creative Diagnostics
1 ml
EUR 864
DiagNano™ Carboxyl Quantum Dot Beads, 120 nm, 585 nm Emission Peak
DNQ-N004
Creative Diagnostics
1 ml
EUR 864
DiagNano™ Carboxyl Quantum Dot Beads, 140 nm, 565 nm Emission Peak
DNQ-N005
Creative Diagnostics
1 ml
EUR 864
DiagNano™ Carboxyl Quantum Dot Beads, 160 nm, 525 nm Emission Peak
DNQ-N006
Creative Diagnostics
1 ml
EUR 864
Tide Fluorâ„¢ 8WS maleimide [TF8WS maleimide] *Near Infrared Emission*
2337
AAT Bioquest
1 mg
EUR 227
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.
Tide Fluorâ„¢ 8WS maleimide [TF8WS maleimide] *Near Infrared Emission*
2337-1mg
AAT Bioquest
1 mg
EUR 222
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.
Tide Fluorâ„¢ 8WS, succinimidyl ester [TF8WS SE]*Near Infrared Emission*
2338
AAT Bioquest
1 mg
EUR 112
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.
Tide Fluorâ„¢ 8WS, succinimidyl ester [TF8WS SE]*Near Infrared Emission*
2338-1mg
AAT Bioquest
1 mg
EUR 109
Description: Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800.
DAPI
abx283157-100g
Abbexa
100 µg
Ask for price
DAPI
abx283157-20g
Abbexa
20 µg
EUR 106.25
DAPI
abx283157-50g
Abbexa
50 µg
EUR 237.5
DAPI
BMD0063-100mg
Abbkine
100 mg
EUR 849
Description: DAPI is a popular blue fluorescent DNA dye.
DAPI
BMD0063-10mg
Abbkine
10 mg
EUR 119
Description: DAPI is a popular blue fluorescent DNA dye.
DAPI
BMD0063-each
Abbkine
each
Ask for price
DAPI
abx082063-100l
Abbexa
100 µl
EUR 212.5
DAPI
abx082063-10mg
Abbexa
10 mg
EUR 276
DAPI
abx082063-1ml
Abbexa
1 ml
Ask for price
DAPI
abx082063-200l
Abbexa
200 µl
Ask for price
DAPI Reagent
MBS2567122-100Tests
MyBiosource
100Tests
EUR 85
DAPI Reagent
MBS2567122-200Tests
MyBiosource
200Tests
EUR 95
DAPI Reagent
MBS2567122-500Tests
MyBiosource
500Tests
EUR 115
DAPI Reagent
MBS2567122-50Tests
MyBiosource
50Tests
EUR 80
DAPI Reagent
MBS2567122-5x500Tests
MyBiosource
5x500Tests
EUR 510
DAPI Reagent
MBS2563514-5x50mL
MyBiosource
5x50mL
EUR 640
DAPI Dilactate
17509
AAT Bioquest
25 mg
EUR 112
Description: DAPI is a fluorescent stain that binds strongly to DNA.
DAPI Dilactate
17509-25mg
AAT Bioquest
25 mg
EUR 109
Description: DAPI is a fluorescent stain that binds strongly to DNA.
DAPI (hydrochloride)
C3362-10
ApexBio
10 mg
EUR 64
Description: Probes & Dyes|Nuclear Stains
DAPI (hydrochloride)
C3362-25
ApexBio
25 mg
EUR 130.4
Description: Probes & Dyes|Nuclear Stains
DAPI (hydrochloride)
C3362-5
ApexBio
5 mg
EUR 40
Description: Probes & Dyes|Nuclear Stains
DAPI (Hydrochloride)
MBS696332-25mg
MyBiosource
25mg
EUR 260
DAPI (Hydrochloride)
MBS696332-5mg
MyBiosource
5mg
EUR 150
DAPI (Hydrochloride)
MBS696332-5x25mg
MyBiosource
5x25mg
EUR 1120
DAPI dihydrochloride
MB097-10MG
EWC Diagnostics
1 unit
EUR 41.98
Description: DAPI dihydrochloride
DAPI dihydrochloride
MB097-25MG
EWC Diagnostics
1 unit
EUR 83.31
Description: DAPI dihydrochloride
DAPI Dihydrochloride
MBS5756233-100mg
MyBiosource
100mg
EUR 390
DAPI Dihydrochloride
MBS5756233-10mg
MyBiosource
10mg
EUR 165
DAPI Dihydrochloride
MBS5756233-25mg
MyBiosource
25mg
EUR 225
DAPI Dihydrochloride
MBS5756233-50mg
MyBiosource
50mg
EUR 315
DAPI Dihydrochloride
MBS5756233-5mg
MyBiosource
5mg
EUR 155
DAPI Dihydrochloride
T19827-10mg
TargetMol Chemicals
10mg
Ask for price
Description: DAPI Dihydrochloride
DAPI Dihydrochloride
T19827-1g
TargetMol Chemicals
1g
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Description: DAPI Dihydrochloride
DAPI Dihydrochloride
T19827-1mg
TargetMol Chemicals
1mg
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Description: DAPI Dihydrochloride
DAPI Dihydrochloride
T19827-50mg
TargetMol Chemicals
50mg
Ask for price
Description: DAPI Dihydrochloride
DAPI Dihydrochloride
T19827-5mg
TargetMol Chemicals
5mg
Ask for price
Description: DAPI Dihydrochloride
DAPI dihydrochloride
RM5773-25MG
EWC Diagnostics
1 unit
EUR 119.85
Description: DAPI dihydrochloride
DAPI Staining Kit
abx097206-100l
Abbexa
100 µl
EUR 162.5
DAPI Staining Kit
abx097206-1Kit
Abbexa
1 Kit
EUR 276
DAPI Staining Kit
abx097206-1ml
Abbexa
1 ml
Ask for price
DAPI Staining Kit
abx097206-200l
Abbexa
200 µl
Ask for price
DAPI Staining Kit
MBS826728-01mL
MyBiosource
0.1mL
EUR 140
DAPI Staining Kit
MBS826728-5x01mL
MyBiosource
5x0.1mL
EUR 550
DAPI solution(0.25mM)
E37F17510-50-mL
EnoGene
50 mL
EUR 153
Description: Cell staining. Nuclear staining.
DAPI solution(0.25mM)
E37F17510-5x10-mL
EnoGene
5×10 mL
EUR 173
Description: Cell staining. Nuclear staining.
FluorLast? with DAPI
1214-20
Biovision
each
EUR 183.6
DAPI Staining Solution
MBS176580-10mL
MyBiosource
10mL
EUR 115
DAPI Staining Solution
MBS176580-50mL
MyBiosource
50mL
EUR 135
DAPI Staining Solution
MBS176580-5x50mL
MyBiosource
5x50mL
EUR 450
Dapi-Fluoromount-G
MBS679125-20mL
MyBiosource
20mL
EUR 150
Dapi-Fluoromount-G
MBS679125-5x20mL
MyBiosource
5x20mL
EUR 535
DAPI solution(1mg/ml)
E37F17510DZ-1-mL
EnoGene
1 mL
EUR 60
Description: Cell staining. Nuclear staining.
DAPI Led Filter Cube
LBI10101
Westburg
each
EUR 2735.9
DAPI solution (1mg/ml)
MBS8579913-1mL
MyBiosource
1mL
EUR 140
DAPI solution (1mg/ml)
MBS8579913-5x1mL
MyBiosource
5x1mL
EUR 570
DAPI-solution(1mg/ml)
E37F17510DZ
EnoGene
1-mL
EUR 60
Description: Cell-staining.-Nuclear-staining.
DAPI (Molecular Biology Grade)
CE117
GeneOn
5 mg
EUR 40
DAPI (Molecular Biology Grade)
CE118
GeneOn
25 mg
EUR 133
DAPI (Molecular Biology Grade)
CE119
GeneOn
100 mg
EUR 281
DAPI, 10mg/mL in H2O
40043-1
Biotium
EA
EUR 103
DAPI (Ex375/28, Em460/50)
LBI10130
Westburg
each
EUR 3270
Fluoroshield with DAPI & Propylgallate
AR-6506-01
ImmunoBioscience
20 ml
EUR 68.05
Fluoroshield with DAPI & Propylgallate
AR-6506-02
ImmunoBioscience
100 ml
EUR 247.1
FluorLast? with DAPI and DABCO
1218-20
Biovision
each
EUR 183.6
Fluoromount Mounting Medium with DAPI
42734051-1
Bio-WORLD
20 mL
EUR 98.6
FluoroQuestâ„¢ Mounting Medium with DAPI
20004-50mL
AAT Bioquest
50 mL
EUR 109
Description: FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.
FluoroQuestâ„¢ Mounting Medium with DAPI
20004
AAT Bioquest
50 mL
EUR 112
Description: FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.
DAPI staining kit (ready to use)
EGY0621
EnoGene
10mL
EUR 85
DAPI staining kit (ready to use)
EGY0622
EnoGene
50mL
EUR 185
DAPI staining kit (ready to use)
MBS8584952-10mL
MyBiosource
10mL
EUR 200
DAPI staining kit (ready to use)
MBS8584952-5x10mL
MyBiosource
5x10mL
EUR 915
DAPI staining kit (ready to use)
MBS8584953-50mL
MyBiosource
50mL
EUR 285
DAPI staining kit (ready to use)
MBS8584953-5x50mL
MyBiosource
5x50mL
EUR 1300
Annexin V-PE/DAPI Apoptosis Kit
E-CK-A256-100Assays
Elabscience Biotech
100 Assays
EUR 260
Description: Apoptosis/Annexin V
Annexin V-PE/DAPI Apoptosis Kit
E-CK-A256-200Assays
Elabscience Biotech
200 Assays
EUR 398
Description: Apoptosis/Annexin V
Annexin V-PE/DAPI Apoptosis Kit
E-CK-A256-20Assays
Elabscience Biotech
20 Assays
EUR 75
Description: Apoptosis/Annexin V
Annexin V-PE/DAPI Apoptosis Kit
E-CK-A256-50Assays
Elabscience Biotech
50 Assays
EUR 150
Description: Apoptosis/Annexin V
Annexin V-APC/DAPI Apoptosis Kit
E-CK-A258-100Assays
Elabscience Biotech
100 Assays
EUR 260
Description: Apoptosis/Annexin V
Annexin V-APC/DAPI Apoptosis Kit
E-CK-A258-200Assays
Elabscience Biotech
200 Assays
EUR 398
Description: Apoptosis/Annexin V
Annexin V-APC/DAPI Apoptosis Kit
E-CK-A258-20Assays
Elabscience Biotech
20 Assays
EUR 75
Description: Apoptosis/Annexin V
Annexin V-APC/DAPI Apoptosis Kit
E-CK-A258-50Assays
Elabscience Biotech
50 Assays
EUR 150
Description: Apoptosis/Annexin V
Annexin V-FITC/DAPI Apoptosis Kit
E-CK-A252-100Assays
Elabscience Biotech
100 Assays
EUR 260
Description: Apoptosis/Annexin V
Annexin V-FITC/DAPI Apoptosis Kit
E-CK-A252-200Assays
Elabscience Biotech
200 Assays
EUR 398
Description: Apoptosis/Annexin V
Annexin V-FITC/DAPI Apoptosis Kit
E-CK-A252-20Assays
Elabscience Biotech
20 Assays
EUR 75
Description: Apoptosis/Annexin V
Annexin V-FITC/DAPI Apoptosis Kit
E-CK-A252-50Assays
Elabscience Biotech
50 Assays
EUR 150
Description: Apoptosis/Annexin V
Annexin V-EGFP/DAPI Apoptosis Kit
E-CK-A260-100Assays
Elabscience Biotech
100 Assays
EUR 260
Description: Apoptosis/Annexin V
Annexin V-EGFP/DAPI Apoptosis Kit
E-CK-A260-200Assays
Elabscience Biotech
200 Assays
EUR 398
Description: Apoptosis/Annexin V
Annexin V-EGFP/DAPI Apoptosis Kit
E-CK-A260-20Assays
Elabscience Biotech
20 Assays
EUR 75
Description: Apoptosis/Annexin V
Annexin V-EGFP/DAPI Apoptosis Kit
E-CK-A260-50Assays
Elabscience Biotech
50 Assays
EUR 150
Description: Apoptosis/Annexin V
Anti-Fade Mounting Medium (With DAPI)
abx090690-100l
Abbexa
100 µl
EUR 137.5
Anti-Fade Mounting Medium (With DAPI)
abx090690-10ml
Abbexa
10 ml
EUR 243.6
Anti-Fade Mounting Medium (With DAPI)
abx090690-1ml
Abbexa
1 ml
Ask for price
Anti-Fade Mounting Medium (With DAPI)
abx090690-200l
Abbexa
200 µl
Ask for price
Anti-fade Mounting Medium (With DAPI)
MBS355738-10mL
MyBiosource
10mL
EUR 180
Anti-fade Mounting Medium (With DAPI)
MBS355738-5x10mL
MyBiosource
5x10mL
EUR 515
DAPI blue fluorescent nuclear DNA dye
MBS258073-10mg
MyBiosource
10mg
EUR 245
DAPI blue fluorescent nuclear DNA dye
MBS258073-5x10mg
MyBiosource
5x10mg
EUR 930
EverBrite™ Mounting Medium with DAPI
23002-1
Biotium
EA
EUR 172
Annexin V-Cyanine5/DAPI Apoptosis Kit
E-CK-A262-100Assays
Elabscience Biotech
100 Assays
EUR 260
Description: Apoptosis/Annexin V
Annexin V-Cyanine5/DAPI Apoptosis Kit
E-CK-A262-200Assays
Elabscience Biotech
200 Assays
EUR 398
Description: Apoptosis/Annexin V
Annexin V-Cyanine5/DAPI Apoptosis Kit
E-CK-A262-20Assays
Elabscience Biotech
20 Assays
EUR 75
Description: Apoptosis/Annexin V
Annexin V-Cyanine5/DAPI Apoptosis Kit
E-CK-A262-50Assays
Elabscience Biotech
50 Assays
EUR 150
Description: Apoptosis/Annexin V
Enhanced Antifade Mounting Medium with DAPI
MBS9719255-10mL
MyBiosource
10mL
EUR 115
Enhanced Antifade Mounting Medium with DAPI
MBS9719255-50mL
MyBiosource
50mL
EUR 140
Enhanced Antifade Mounting Medium with DAPI
MBS9719255-5x10mL
MyBiosource
5x10mL
EUR 500